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The map label for this gene is def

Identifier: 146300287

GI number: 146300287

Start: 3015968

End: 3016558

Strand: Direct

Name: def

Synonym: Fjoh_2532

Alternate gene names: 146300287

Gene position: 3015968-3016558 (Clockwise)

Preceding gene: 146300285

Following gene: 146300288

Centisome position: 49.47

GC content: 34.35

Gene sequence:

>591_bases
ATGATTTTACCAATTGTAGGATATGGTGATCCTGTTTTAAGAAAAGTAGGTACGGCAATTACGCCAGATTATCCTAACTT
AAAAGAGACAATAGCAAACATGTATGAAACCATGTATAATGCTTACGGAGTTGGACTTGCTGCACCGCAGGTAGGTTTGC
CAATACGTATATTTGTTATCGATACAACACCTTTTAGTGATGACGAGGATTTGCCGGCAGATGAACAAAAGGATTTAAAA
GGTTTTAAAAGAACTTTTATCAATGCTAAAATTGTTAAAGAAGAAGGTGAGGAGTGGAGTTTTAACGAAGGATGTTTAAG
TATTCCGGATGTTCGTGAAGATGTTTACAGAAAACCAACTGTTACAATTGAATACTGCGAAGAAGATTTTGTAATGAAAA
CAGAAGTTTTTGACGGTTTAATTGCAAGAGTAATTCAACACGAATACGACCACATCGAAGGAGTTTTATTTACAGATAAA
ATATCTTCTTTGAAAAAGCGTTTGATTCAAAAGAAATTGAAAAATATCACAGAAGGCAAAACGTTTCAGGAATACAGAAT
GAAATTTGCAGCCGCTAAAAAAGGCAGATAA

Upstream 100 bases:

>100_bases
GTTTACAACTATTGGTTGACTATTTTTTACTATTTTTGCCAGCTGAATTATGAGAACCGATTTTTAAGGTTTTCGTTAGA
AAAACATTAAATAAAAACAA

Downstream 100 bases:

>100_bases
TGTTCTAAAAAAAGAACATCAAAAAAATACTAGAAATTCTTAATACTAATTTTAATAAACATGAATTTAGACAAAATTTT
AGCCATTTCTGGGAAACCAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MILPIVGYGDPVLRKVGTAITPDYPNLKETIANMYETMYNAYGVGLAAPQVGLPIRIFVIDTTPFSDDEDLPADEQKDLK
GFKRTFINAKIVKEEGEEWSFNEGCLSIPDVREDVYRKPTVTIEYCEEDFVMKTEVFDGLIARVIQHEYDHIEGVLFTDK
ISSLKKRLIQKKLKNITEGKTFQEYRMKFAAAKKGR

Sequences:

>Translated_196_residues
MILPIVGYGDPVLRKVGTAITPDYPNLKETIANMYETMYNAYGVGLAAPQVGLPIRIFVIDTTPFSDDEDLPADEQKDLK
GFKRTFINAKIVKEEGEEWSFNEGCLSIPDVREDVYRKPTVTIEYCEEDFVMKTEVFDGLIARVIQHEYDHIEGVLFTDK
ISSLKKRLIQKKLKNITEGKTFQEYRMKFAAAKKGR
>Mature_196_residues
MILPIVGYGDPVLRKVGTAITPDYPNLKETIANMYETMYNAYGVGLAAPQVGLPIRIFVIDTTPFSDDEDLPADEQKDLK
GFKRTFINAKIVKEEGEEWSFNEGCLSIPDVREDVYRKPTVTIEYCEEDFVMKTEVFDGLIARVIQHEYDHIEGVLFTDK
ISSLKKRLIQKKLKNITEGKTFQEYRMKFAAAKKGR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=183, Percent_Identity=33.879781420765, Blast_Score=95, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645728, Length=178, Percent_Identity=28.0898876404494, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_FLAJ1 (A5FGV5)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194878.1
- ProteinModelPortal:   A5FGV5
- SMR:   A5FGV5
- STRING:   A5FGV5
- GeneID:   5091986
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2532
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   HLESEDQ
- ProtClustDB:   PRK00150
- BioCyc:   FJOH376686:FJOH_2532-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22410; Mature: 22410

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPIVGYGDPVLRKVGTAITPDYPNLKETIANMYETMYNAYGVGLAAPQVGLPIRIFVI
CEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
DTTPFSDDEDLPADEQKDLKGFKRTFINAKIVKEEGEEWSFNEGCLSIPDVREDVYRKPT
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCC
VTIEYCEEDFVMKTEVFDGLIARVIQHEYDHIEGVLFTDKISSLKKRLIQKKLKNITEGK
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCCC
TFQEYRMKFAAAKKGR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILPIVGYGDPVLRKVGTAITPDYPNLKETIANMYETMYNAYGVGLAAPQVGLPIRIFVI
CEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
DTTPFSDDEDLPADEQKDLKGFKRTFINAKIVKEEGEEWSFNEGCLSIPDVREDVYRKPT
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHCCCC
VTIEYCEEDFVMKTEVFDGLIARVIQHEYDHIEGVLFTDKISSLKKRLIQKKLKNITEGK
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHCCC
TFQEYRMKFAAAKKGR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA