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The map label for this gene is oprF [H]

Identifier: 146300076

GI number: 146300076

Start: 2788000

End: 2790183

Strand: Direct

Name: oprF [H]

Synonym: Fjoh_2321

Alternate gene names: 146300076

Gene position: 2788000-2790183 (Clockwise)

Preceding gene: 146300065

Following gene: 146300077

Centisome position: 45.73

GC content: 36.54

Gene sequence:

>2184_bases
ATGAAAAAAACATTACTTATTTTATTCTTTTTAATTACATCTTTACAAGCCGAAGCACAATCCTATCTGGGTTATTTTCA
TGATAATTATGCAGGAGTTCAAAGTGTACTTTTTAACCCGGCTTCTATTGCCGATTCTCGTTTTAAAACAGATATTAATT
TGTTTTCTATAAGTGGTTCTGTTCAGAACGATTTATACGGTGTAAAACTTTTTGATGTTTACAAAGACGGGTATGATTTT
GACACGCAGTCAAAAATGAGTCCAAAAAACGCTAACAATGGTCTGGTAAATTTTGATATTATGGGACCATCGTTTATGTT
TAACATTGCGCCAAAACATACTTTGGCAGTATTTACTAGAGCAAGATCAATTGCTAATGTAAACAAGCTTAACGGGTTTC
TTGTAGATCAGGTAAAAGACGGTTTAGACCAGTCGAGTAACTTTAATTACAATTTAGGAAGTCCAAACGCAGCAGCAAAT
GCGTGGGGAGAAGTTGGTATTTCTTATGCTGCAGTTCTATACCAAAACAACCAGCACTTTTTAAAAGGAGGTTTAACTGC
TAAATATCTTCAGGGCGGCGCAAATGCTGATGTTAAAGGAAATAATGTAAGTGTGGCTTATCTTGAAAATAAGGCAAATC
CTAAAGCAGGATCTTTAATTTCAACTGGAGAAATTTCTGTTGGAGGAAGCCAGGATTGGGAAAACAATGAAGATTACGAT
TTTGATGCAGCTTCACGCGGATTTGGTTTTGATTTTGGTTTGGTTTACGAATGGAGACCAGATTACGAAAAGTATGACGT
TAGAAATGCAAAACCTGCAGATAACAACTTTAGAGATCTAAACAAATACAAATTACGTTTCGGGTTATCTGTAACTGATA
TTGGTTCTATCAACTACAAGAATTCAAAAATTGATACTTATAATGTAAATGGAGTTGTTACGCAGCAAATGATTGATGAT
GCTGATGATTTATATGAATTCTTAAACGATCATTATACTAAAGTTTCAAGTTCAAAAGGTGTAAAAACAAACCTGCCAAC
AGCTATTCATGCTGATGTTGACTGGAACATGTACAATAAATTCTATTTGAATCTTAACGGAGATATCAGCATGGTATCTG
AAAACAAATTAAATTCATACGGAATTGCAGACAGAGTTACCTTAACACCTCGTTACGAAAGCAGATGGTTTAGTTTTTAT
GTGCCGGTGACGTACATGGAATACAGCGGTATGCAGGCAGGAGCAGGTTTACGTGCTGGAGTTTTCTTCATTGGTTCTGG
ATCTATTTTGAGCAATTTAGCTTCTAAAGATTCAAAAGGAGCAGATTTTCACCTAGGAATGAAAATTCCGGTTTACGAAA
AACAATTTAAAGATACAGACGGAGACGGTGTAATTGACAAACAAGATGCTTGTAGAAAAGTTGCGGGTCCTGTAGAAAAT
AATGGATGTCCTTGGCCGGATACAGATAAAGACGGTGTTTTTGACAAAGACGACGCTTGCCCGGATACAGCAGGTCCAAA
AGAAAACAAAGGATGTCCGTGGAAAGATTCTGACGGAGATAGTTTATTGGACAATGTAGATGCATGCCCGGCAGTTGCAG
GTCCTGTAGAAAATAAAGGATGCCCATGGCCTGATACAGACGGCGACGGAATTTTAGACAAAGATGATGCATGCCCTAAT
GAAGCAGGATTAGCAGAAAACAAAGGCTGTCCGAGTTTAGATGCTGATAAAGATGGTGTTTTAGATAAAGATGATGACTG
CCCGTTAGTATATGGACCAGCAGACAATAAAGGCTGTCCTAAAGTTACCAAAGAAACATTGGCACAATTGAAAATGGAAG
CAAAATCAATTTTCTTTACAACAGGAAAAGCGACTTTGAGCGATGCTAAAATGGGAGAAACGTCAGGAAGATTAGACGCT
ATTAAAGAAATCCTGAAAAACTATCCAAATGCTAAATTTGCTATTAACGGACACACAGATAATGTAGGTAATGCAAAAGT
AAACCAGAAATTGTCTGAGCAGAGAGCAAAAGTAGTGATGGATCTTTTAATAGAAAAAGGAGTAAATCCTGCTAACTTAA
CTTCAAAAGGTTACGGAGCTTCAAAACCAGTAAAATCAAATAAAACAGCTTCTGGCAGAGCAGAAAACAGAAGAACAGAA
ATTGTTTATTTAGGCAATTTATAA

Upstream 100 bases:

>100_bases
AATTTTTCAAAGATGTTACATCAATTCTATGTTTCCGTAAAGTTTAATGATTTCAATATTGTAGTTTTGCCGCCAGTTCA
AACTCAAAACTCAAATTTTA

Downstream 100 bases:

>100_bases
TCTGTAGATAATATATACCCAAAAAGCCATTCTTAATTGAGTGGCTTTTTTTATTTTTGCATACTTTTTAAAAAGAATCA
TTTCTTTGAAACAGAAGTTC

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MKKTLLILFFLITSLQAEAQSYLGYFHDNYAGVQSVLFNPASIADSRFKTDINLFSISGSVQNDLYGVKLFDVYKDGYDF
DTQSKMSPKNANNGLVNFDIMGPSFMFNIAPKHTLAVFTRARSIANVNKLNGFLVDQVKDGLDQSSNFNYNLGSPNAAAN
AWGEVGISYAAVLYQNNQHFLKGGLTAKYLQGGANADVKGNNVSVAYLENKANPKAGSLISTGEISVGGSQDWENNEDYD
FDAASRGFGFDFGLVYEWRPDYEKYDVRNAKPADNNFRDLNKYKLRFGLSVTDIGSINYKNSKIDTYNVNGVVTQQMIDD
ADDLYEFLNDHYTKVSSSKGVKTNLPTAIHADVDWNMYNKFYLNLNGDISMVSENKLNSYGIADRVTLTPRYESRWFSFY
VPVTYMEYSGMQAGAGLRAGVFFIGSGSILSNLASKDSKGADFHLGMKIPVYEKQFKDTDGDGVIDKQDACRKVAGPVEN
NGCPWPDTDKDGVFDKDDACPDTAGPKENKGCPWKDSDGDSLLDNVDACPAVAGPVENKGCPWPDTDGDGILDKDDACPN
EAGLAENKGCPSLDADKDGVLDKDDDCPLVYGPADNKGCPKVTKETLAQLKMEAKSIFFTTGKATLSDAKMGETSGRLDA
IKEILKNYPNAKFAINGHTDNVGNAKVNQKLSEQRAKVVMDLLIEKGVNPANLTSKGYGASKPVKSNKTASGRAENRRTE
IVYLGNL

Sequences:

>Translated_727_residues
MKKTLLILFFLITSLQAEAQSYLGYFHDNYAGVQSVLFNPASIADSRFKTDINLFSISGSVQNDLYGVKLFDVYKDGYDF
DTQSKMSPKNANNGLVNFDIMGPSFMFNIAPKHTLAVFTRARSIANVNKLNGFLVDQVKDGLDQSSNFNYNLGSPNAAAN
AWGEVGISYAAVLYQNNQHFLKGGLTAKYLQGGANADVKGNNVSVAYLENKANPKAGSLISTGEISVGGSQDWENNEDYD
FDAASRGFGFDFGLVYEWRPDYEKYDVRNAKPADNNFRDLNKYKLRFGLSVTDIGSINYKNSKIDTYNVNGVVTQQMIDD
ADDLYEFLNDHYTKVSSSKGVKTNLPTAIHADVDWNMYNKFYLNLNGDISMVSENKLNSYGIADRVTLTPRYESRWFSFY
VPVTYMEYSGMQAGAGLRAGVFFIGSGSILSNLASKDSKGADFHLGMKIPVYEKQFKDTDGDGVIDKQDACRKVAGPVEN
NGCPWPDTDKDGVFDKDDACPDTAGPKENKGCPWKDSDGDSLLDNVDACPAVAGPVENKGCPWPDTDGDGILDKDDACPN
EAGLAENKGCPSLDADKDGVLDKDDDCPLVYGPADNKGCPKVTKETLAQLKMEAKSIFFTTGKATLSDAKMGETSGRLDA
IKEILKNYPNAKFAINGHTDNVGNAKVNQKLSEQRAKVVMDLLIEKGVNPANLTSKGYGASKPVKSNKTASGRAENRRTE
IVYLGNL
>Mature_727_residues
MKKTLLILFFLITSLQAEAQSYLGYFHDNYAGVQSVLFNPASIADSRFKTDINLFSISGSVQNDLYGVKLFDVYKDGYDF
DTQSKMSPKNANNGLVNFDIMGPSFMFNIAPKHTLAVFTRARSIANVNKLNGFLVDQVKDGLDQSSNFNYNLGSPNAAAN
AWGEVGISYAAVLYQNNQHFLKGGLTAKYLQGGANADVKGNNVSVAYLENKANPKAGSLISTGEISVGGSQDWENNEDYD
FDAASRGFGFDFGLVYEWRPDYEKYDVRNAKPADNNFRDLNKYKLRFGLSVTDIGSINYKNSKIDTYNVNGVVTQQMIDD
ADDLYEFLNDHYTKVSSSKGVKTNLPTAIHADVDWNMYNKFYLNLNGDISMVSENKLNSYGIADRVTLTPRYESRWFSFY
VPVTYMEYSGMQAGAGLRAGVFFIGSGSILSNLASKDSKGADFHLGMKIPVYEKQFKDTDGDGVIDKQDACRKVAGPVEN
NGCPWPDTDKDGVFDKDDACPDTAGPKENKGCPWKDSDGDSLLDNVDACPAVAGPVENKGCPWPDTDGDGILDKDDACPN
EAGLAENKGCPSLDADKDGVLDKDDDCPLVYGPADNKGCPKVTKETLAQLKMEAKSIFFTTGKATLSDAKMGETSGRLDA
IKEILKNYPNAKFAINGHTDNVGNAKVNQKLSEQRAKVVMDLLIEKGVNPANLTSKGYGASKPVKSNKTASGRAENRRTE
IVYLGNL

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=105, Percent_Identity=37.1428571428571, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788957, Length=69, Percent_Identity=50.7246376811594, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 79474; Mature: 79474

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLLILFFLITSLQAEAQSYLGYFHDNYAGVQSVLFNPASIADSRFKTDINLFSISGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCHHCCCEEEEEECCC
VQNDLYGVKLFDVYKDGYDFDTQSKMSPKNANNGLVNFDIMGPSFMFNIAPKHTLAVFTR
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHH
ARSIANVNKLNGFLVDQVKDGLDQSSNFNYNLGSPNAAANAWGEVGISYAAVLYQNNQHF
HHHHHHHHHHCCEEHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHCCEEEEEEEECCCCE
LKGGLTAKYLQGGANADVKGNNVSVAYLENKANPKAGSLISTGEISVGGSQDWENNEDYD
ECCCCEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCEEECCCCCCCCCCCCCC
FDAASRGFGFDFGLVYEWRPDYEKYDVRNAKPADNNFRDLNKYKLRFGLSVTDIGSINYK
CCCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCCHHCCEEEEEECCEEEECCCCCCC
NSKIDTYNVNGVVTQQMIDDADDLYEFLNDHYTKVSSSKGVKTNLPTAIHADVDWNMYNK
CCCEEEEECCCEEHHHHHCCHHHHHHHHHHHHHECCCCCCCCCCCCEEEEECCCCCEEEE
FYLNLNGDISMVSENKLNSYGIADRVTLTPRYESRWFSFYVPVTYMEYSGMQAGAGLRAG
EEEEECCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCEEE
VFFIGSGSILSNLASKDSKGADFHLGMKIPVYEKQFKDTDGDGVIDKQDACRKVAGPVEN
EEEEECCHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
NGCPWPDTDKDGVFDKDDACPDTAGPKENKGCPWKDSDGDSLLDNVDACPAVAGPVENKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
CPWPDTDGDGILDKDDACPNEAGLAENKGCPSLDADKDGVLDKDDDCPLVYGPADNKGCP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
KVTKETLAQLKMEAKSIFFTTGKATLSDAKMGETSGRLDAIKEILKNYPNAKFAINGHTD
HHHHHHHHHHHHHHCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCC
NVGNAKVNQKLSEQRAKVVMDLLIEKGVNPANLTSKGYGASKPVKSNKTASGRAENRRTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IVYLGNL
EEEECCC
>Mature Secondary Structure
MKKTLLILFFLITSLQAEAQSYLGYFHDNYAGVQSVLFNPASIADSRFKTDINLFSISGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCHHCCCEEEEEECCC
VQNDLYGVKLFDVYKDGYDFDTQSKMSPKNANNGLVNFDIMGPSFMFNIAPKHTLAVFTR
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHH
ARSIANVNKLNGFLVDQVKDGLDQSSNFNYNLGSPNAAANAWGEVGISYAAVLYQNNQHF
HHHHHHHHHHCCEEHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHCCEEEEEEEECCCCE
LKGGLTAKYLQGGANADVKGNNVSVAYLENKANPKAGSLISTGEISVGGSQDWENNEDYD
ECCCCEEHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEECCEEECCCCCCCCCCCCCC
FDAASRGFGFDFGLVYEWRPDYEKYDVRNAKPADNNFRDLNKYKLRFGLSVTDIGSINYK
CCCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCCHHCCEEEEEECCEEEECCCCCCC
NSKIDTYNVNGVVTQQMIDDADDLYEFLNDHYTKVSSSKGVKTNLPTAIHADVDWNMYNK
CCCEEEEECCCEEHHHHHCCHHHHHHHHHHHHHECCCCCCCCCCCCEEEEECCCCCEEEE
FYLNLNGDISMVSENKLNSYGIADRVTLTPRYESRWFSFYVPVTYMEYSGMQAGAGLRAG
EEEEECCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCEEE
VFFIGSGSILSNLASKDSKGADFHLGMKIPVYEKQFKDTDGDGVIDKQDACRKVAGPVEN
EEEEECCHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
NGCPWPDTDKDGVFDKDDACPDTAGPKENKGCPWKDSDGDSLLDNVDACPAVAGPVENKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
CPWPDTDGDGILDKDDACPNEAGLAENKGCPSLDADKDGVLDKDDDCPLVYGPADNKGCP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
KVTKETLAQLKMEAKSIFFTTGKATLSDAKMGETSGRLDAIKEILKNYPNAKFAINGHTD
HHHHHHHHHHHHHHCEEEEECCCCEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCC
NVGNAKVNQKLSEQRAKVVMDLLIEKGVNPANLTSKGYGASKPVKSNKTASGRAENRRTE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
IVYLGNL
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]