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The map label for this gene is ddh [H]

Identifier: 146299677

GI number: 146299677

Start: 2215912

End: 2216913

Strand: Direct

Name: ddh [H]

Synonym: Fjoh_1917

Alternate gene names: 146299677

Gene position: 2215912-2216913 (Clockwise)

Preceding gene: 146299674

Following gene: 146299678

Centisome position: 36.35

GC content: 33.23

Gene sequence:

>1002_bases
ATGAGCAAAATCGCCTTTTTCTCAACGCAGCCTTACGACAAAGCTTTCTTTAACAAATACAATGCTGACTTTGGTTTTGA
ATTAGATTTTTTCGAAACACAATTGAATCCGCAGACTGTAATTTTAATTGAAAATGCTGAAATTGTCTGTGTTTTCGTAA
ATGATATTGTAAACGAAACAGTAATAAAACAATTGGCAGAAAAAAATGTAAAAATTATTGCATTGCGATGTGCGGGTTTT
AATAATGTTGATCTGGATGCTGCAAAAAAGTACTGCCTTAAAGTCTGCCGTGTTCCTGCATATTCTCCACAAGCCGTTGC
AGAACATGCAATGGCCATGATTTTGACTTTAAACAGAAAAACACATAAAGCTTATAACAGAGTTCGCGAACAGAATTTTT
CTCTAAACGGATTATTGGGGTTTGATTTGTTTGGAAAGACAATTGGAATTATTGGAACAGGAAATATAGGAAAAGCATTT
TCTAAAATTGCTCTTGGTTTTGGCTGTAAAGTTTTGGCTTATGATATTGTTGAAAATGAAGAAATGAAAAAGGAAGGCGT
ATCTTTTGTTGGTTTAGAGGAGATTTTTAAATCAAGTGATATTATTTCGCTTCACTGCCCATTAAACGATCAGACGAAGC
ATGTGATTAATAAGACTTCGATTTCTTTTATGAAAGATAATGTAATGATTATCAATACAAGCCGCGGCGGATTAATAGAA
ACTTCGAGTGTAATTGAAGGTTTAAAAGAAGGTAAAATAGGTTATTTAGGGATTGATGTTTACGAACAGGAAGAAAAACT
GTTCTTTAGAGATTTATCAGCAGATATTATTCAGGATGATGCAATTCAGCGTTTAATGAGTTTTCCAAATGTTTTGGTAA
CGGCGCATCAGGCATTTTTTACTAATGAAGCTTTAACCCAGATCGCTTTGGTGACTTTTAATAATATAAAATCATTGCTG
GAGAAAAACGATATTGAAAATAAAACAGCTCTGCTGGTTTAA

Upstream 100 bases:

>100_bases
ATTGTTTATTTAACTTTTGAAAGAAGTAGTTTATGAAAGTTAACATTGAAAATATTTTCTTATTTTTAAGGTATCAACAA
ATTAAAATCCAGAAAACATT

Downstream 100 bases:

>100_bases
ATTATGTAGGCGTAAACTCGGTTTCAAAAAACTGCTAAGTTTGGAAGGAATTTATAATTTAAAAAATCAGATTATGAGAA
ATAAAATCCTAATTCTAGTT

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSKIAFFSTQPYDKAFFNKYNADFGFELDFFETQLNPQTVILIENAEIVCVFVNDIVNETVIKQLAEKNVKIIALRCAGF
NNVDLDAAKKYCLKVCRVPAYSPQAVAEHAMAMILTLNRKTHKAYNRVREQNFSLNGLLGFDLFGKTIGIIGTGNIGKAF
SKIALGFGCKVLAYDIVENEEMKKEGVSFVGLEEIFKSSDIISLHCPLNDQTKHVINKTSISFMKDNVMIINTSRGGLIE
TSSVIEGLKEGKIGYLGIDVYEQEEKLFFRDLSADIIQDDAIQRLMSFPNVLVTAHQAFFTNEALTQIALVTFNNIKSLL
EKNDIENKTALLV

Sequences:

>Translated_333_residues
MSKIAFFSTQPYDKAFFNKYNADFGFELDFFETQLNPQTVILIENAEIVCVFVNDIVNETVIKQLAEKNVKIIALRCAGF
NNVDLDAAKKYCLKVCRVPAYSPQAVAEHAMAMILTLNRKTHKAYNRVREQNFSLNGLLGFDLFGKTIGIIGTGNIGKAF
SKIALGFGCKVLAYDIVENEEMKKEGVSFVGLEEIFKSSDIISLHCPLNDQTKHVINKTSISFMKDNVMIINTSRGGLIE
TSSVIEGLKEGKIGYLGIDVYEQEEKLFFRDLSADIIQDDAIQRLMSFPNVLVTAHQAFFTNEALTQIALVTFNNIKSLL
EKNDIENKTALLV
>Mature_332_residues
SKIAFFSTQPYDKAFFNKYNADFGFELDFFETQLNPQTVILIENAEIVCVFVNDIVNETVIKQLAEKNVKIIALRCAGFN
NVDLDAAKKYCLKVCRVPAYSPQAVAEHAMAMILTLNRKTHKAYNRVREQNFSLNGLLGFDLFGKTIGIIGTGNIGKAFS
KIALGFGCKVLAYDIVENEEMKKEGVSFVGLEEIFKSSDIISLHCPLNDQTKHVINKTSISFMKDNVMIINTSRGGLIET
SSVIEGLKEGKIGYLGIDVYEQEEKLFFRDLSADIIQDDAIQRLMSFPNVLVTAHQAFFTNEALTQIALVTFNNIKSLLE
KNDIENKTALLV

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=246, Percent_Identity=30.0813008130081, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4557497, Length=246, Percent_Identity=30.0813008130081, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI145580578, Length=246, Percent_Identity=29.6747967479675, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI4557499, Length=246, Percent_Identity=29.6747967479675, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI145580575, Length=259, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI23308577, Length=243, Percent_Identity=27.5720164609054, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI6912396, Length=294, Percent_Identity=25.5102040816327, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1787645, Length=317, Percent_Identity=52.6813880126183, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI87082289, Length=298, Percent_Identity=28.8590604026846, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789279, Length=268, Percent_Identity=31.3432835820896, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788660, Length=269, Percent_Identity=24.907063197026, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=248, Percent_Identity=27.8225806451613, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=248, Percent_Identity=28.6290322580645, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=292, Percent_Identity=28.4246575342466, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=249, Percent_Identity=30.9236947791165, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=294, Percent_Identity=27.5510204081633, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=216, Percent_Identity=29.1666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=142, Percent_Identity=29.5774647887324, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI62472511, Length=267, Percent_Identity=31.4606741573034, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19921140, Length=243, Percent_Identity=27.9835390946502, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28574286, Length=295, Percent_Identity=26.7796610169492, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585514, Length=257, Percent_Identity=25.6809338521401, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28574282, Length=257, Percent_Identity=25.6809338521401, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45552429, Length=257, Percent_Identity=25.6809338521401, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28574284, Length=257, Percent_Identity=25.6809338521401, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45551003, Length=257, Percent_Identity=25.6809338521401, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28571528, Length=313, Percent_Identity=24.6006389776358, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585516, Length=259, Percent_Identity=24.3243243243243, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37257; Mature: 37125

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIAFFSTQPYDKAFFNKYNADFGFELDFFETQLNPQTVILIENAEIVCVFVNDIVNET
CCCEEEECCCCCHHHHHHCCCCCCCEEEEHHECCCCCEEEEEEECCCEEEEEHHHHHHHH
VIKQLAEKNVKIIALRCAGFNNVDLDAAKKYCLKVCRVPAYSPQAVAEHAMAMILTLNRK
HHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCH
THKAYNRVREQNFSLNGLLGFDLFGKTIGIIGTGNIGKAFSKIALGFGCKVLAYDIVENE
HHHHHHHHHHCCCCCCCEEEHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEHHCCH
EMKKEGVSFVGLEEIFKSSDIISLHCPLNDQTKHVINKTSISFMKDNVMIINTSRGGLIE
HHHHHCCCEECHHHHHCCCCEEEEECCCCCCHHHHHHHHHHEEEECCEEEEECCCCCEEE
TSSVIEGLKEGKIGYLGIDVYEQEEKLFFRDLSADIIQDDAIQRLMSFPNVLVTAHQAFF
HHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHH
TNEALTQIALVTFNNIKSLLEKNDIENKTALLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure 
SKIAFFSTQPYDKAFFNKYNADFGFELDFFETQLNPQTVILIENAEIVCVFVNDIVNET
CCEEEECCCCCHHHHHHCCCCCCCEEEEHHECCCCCEEEEEEECCCEEEEEHHHHHHHH
VIKQLAEKNVKIIALRCAGFNNVDLDAAKKYCLKVCRVPAYSPQAVAEHAMAMILTLNRK
HHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCH
THKAYNRVREQNFSLNGLLGFDLFGKTIGIIGTGNIGKAFSKIALGFGCKVLAYDIVENE
HHHHHHHHHHCCCCCCCEEEHHHHCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEHHCCH
EMKKEGVSFVGLEEIFKSSDIISLHCPLNDQTKHVINKTSISFMKDNVMIINTSRGGLIE
HHHHHCCCEECHHHHHCCCCEEEEECCCCCCHHHHHHHHHHEEEECCEEEEECCCCCEEE
TSSVIEGLKEGKIGYLGIDVYEQEEKLFFRDLSADIIQDDAIQRLMSFPNVLVTAHQAFF
HHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHH
TNEALTQIALVTFNNIKSLLEKNDIENKTALLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]