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The map label for this gene is hemL

Identifier: 146299674

GI number: 146299674

Start: 2213071

End: 2214357

Strand: Direct

Name: hemL

Synonym: Fjoh_1914

Alternate gene names: 146299674

Gene position: 2213071-2214357 (Clockwise)

Preceding gene: 146299673

Following gene: 146299677

Centisome position: 36.3

GC content: 38.31

Gene sequence:

>1287_bases
ATGTTATATAAAAGAAGTAGTCAGCTTTTTGCTGAAGCAGAAAAAGTAATTCCGGGAGGAGTAAATTCACCAGTAAGAGC
CTTTAAAGCAGTTGGCGGAACTCCGATTTTTGTAAAAAGTGCTAAAGGCGCTTATTTATATGATGAAGACGGAAACAAAT
TAATAGATTACATCAACTCTTGGGGACCAATGGTTTTAGGCCACGCTTATCAGCCGGTTGTTGATGCTGTAATCGAAAAA
GCAAAACTGGGTACTTCATTTGGAATGCCGACAGAATTGGAAACAGAAATCGCTGCTTTAGCCGTTTCTATGGTTCCAAA
TATTGACAAAATACGTTTTGTAAATTCGGGTACAGAAGCTTGTATGAGCGCAATTCGTCTGGCTCGCGGATTTACAAAAA
GAGATAAAATCATCAAATTTGCAGGTTGCTATCACGGACATTCTGATTCATTCCTGATTCAGGCAGGAAGCGGTGCGGTA
ACTTTTGGATCTCCAAATAGTCCAGGGGTTACAGAAGGAACTGCAAAAGATACTTTATTAGCGAAATACAATGATTTAGA
GAATGTAAAAACTTTAATCGAAGCTAACAAAGGGGAAATTGCCGCTATCATTATTGAAGCTGTTGCTGGAAATATGGGTT
GTATTCCACCAGCAGAAGGTTTCTTGCAAGGTTTAAGAGACTTATGTACAGCAAACGGAATTTTATTGATTTTTGATGAG
GTAATGACTGGTTTCCGTTTAGCACGCGGCGGAGTTCAGGAATTATATGGAATTGATGCTGATATTGTGACTTTCGGAAA
AGTTATTGGCGGCGGACTTCCTGTTGGAGCTTTTGCAGCACGCGAAGAAATCATGAATTATTTAGCTCCTCTTGGACCAG
TTTATCAGGCAGGAACATTATCTGGAAATCCGTTAGCAATGGCTGCAGGATATGCGATGTTAAAAGCTCTTGATAATGAC
AGAGAAATTTTTACTCGTTTAGAAGAAAAAACAGCATATTTAGAAGCAGGAATTGACAGAGTTTTAAAAGCAAACAATGT
TGTGTTTACAATCAATAGAGTCGGTTCTATGATTTCAGTTCACTTTGATGCAGGTCCGGTGACAGATTTTAAAACTGCTG
CAAAAGGAGATAACGAAACGTTTAAGAAATTCTTCCACGGATTGCTGCAGGAAGGTGTATACATTGCGCCATCTGCATAT
GAAACATGGTTTATTACAGATGCTTTAACGTATGAAGATTTAGATTTTACAATTAATGCGATCGACAAGGTTTCGAAAAC
ATTTTAA

Upstream 100 bases:

>100_bases
CTAACTGATAATGCAATTTCGATTGGGCAGAGGTTGAAAGTGAAATAATTATCAATTGATAATTGACAATTAACAATTAT
CAAGAAATCTAAAATAAAAA

Downstream 100 bases:

>100_bases
TAACAAAAAAAGAGGCTTTTAAAGCCTCTTTTTTTATGGATTATTTTTTGTCTTTTCTGTGTTCTTTCATTTTGTCTTTA
TGCTCTTTTTGAATTGTGGT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLYKRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGAYLYDEDGNKLIDYINSWGPMVLGHAYQPVVDAVIEK
AKLGTSFGMPTELETEIAALAVSMVPNIDKIRFVNSGTEACMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAV
TFGSPNSPGVTEGTAKDTLLAKYNDLENVKTLIEANKGEIAAIIIEAVAGNMGCIPPAEGFLQGLRDLCTANGILLIFDE
VMTGFRLARGGVQELYGIDADIVTFGKVIGGGLPVGAFAAREEIMNYLAPLGPVYQAGTLSGNPLAMAAGYAMLKALDND
REIFTRLEEKTAYLEAGIDRVLKANNVVFTINRVGSMISVHFDAGPVTDFKTAAKGDNETFKKFFHGLLQEGVYIAPSAY
ETWFITDALTYEDLDFTINAIDKVSKTF

Sequences:

>Translated_428_residues
MLYKRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGAYLYDEDGNKLIDYINSWGPMVLGHAYQPVVDAVIEK
AKLGTSFGMPTELETEIAALAVSMVPNIDKIRFVNSGTEACMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAV
TFGSPNSPGVTEGTAKDTLLAKYNDLENVKTLIEANKGEIAAIIIEAVAGNMGCIPPAEGFLQGLRDLCTANGILLIFDE
VMTGFRLARGGVQELYGIDADIVTFGKVIGGGLPVGAFAAREEIMNYLAPLGPVYQAGTLSGNPLAMAAGYAMLKALDND
REIFTRLEEKTAYLEAGIDRVLKANNVVFTINRVGSMISVHFDAGPVTDFKTAAKGDNETFKKFFHGLLQEGVYIAPSAY
ETWFITDALTYEDLDFTINAIDKVSKTF
>Mature_428_residues
MLYKRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGAYLYDEDGNKLIDYINSWGPMVLGHAYQPVVDAVIEK
AKLGTSFGMPTELETEIAALAVSMVPNIDKIRFVNSGTEACMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAV
TFGSPNSPGVTEGTAKDTLLAKYNDLENVKTLIEANKGEIAAIIIEAVAGNMGCIPPAEGFLQGLRDLCTANGILLIFDE
VMTGFRLARGGVQELYGIDADIVTFGKVIGGGLPVGAFAAREEIMNYLAPLGPVYQAGTLSGNPLAMAAGYAMLKALDND
REIFTRLEEKTAYLEAGIDRVLKANNVVFTINRVGSMISVHFDAGPVTDFKTAAKGDNETFKKFFHGLLQEGVYIAPSAY
ETWFITDALTYEDLDFTINAIDKVSKTF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=301, Percent_Identity=29.2358803986711, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI37574042, Length=307, Percent_Identity=30.9446254071661, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI226442705, Length=307, Percent_Identity=30.6188925081433, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI13994255, Length=343, Percent_Identity=30.0291545189504, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI24119277, Length=307, Percent_Identity=27.6872964169381, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI226442709, Length=276, Percent_Identity=30.7971014492754, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI284507298, Length=219, Percent_Identity=30.5936073059361, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1786349, Length=422, Percent_Identity=54.739336492891, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1788044, Length=313, Percent_Identity=31.629392971246, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789016, Length=374, Percent_Identity=28.3422459893048, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789759, Length=386, Percent_Identity=26.4248704663212, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787560, Length=373, Percent_Identity=30.2949061662198, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI145693181, Length=284, Percent_Identity=31.6901408450704, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786991, Length=394, Percent_Identity=26.1421319796954, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71992977, Length=354, Percent_Identity=31.638418079096, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144271, Length=303, Percent_Identity=28.0528052805281, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=207, Percent_Identity=27.536231884058, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=352, Percent_Identity=26.4204545454545, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=360, Percent_Identity=26.1111111111111, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161085790, Length=364, Percent_Identity=28.021978021978, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28574759, Length=364, Percent_Identity=28.021978021978, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21356575, Length=314, Percent_Identity=28.9808917197452, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357415, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_FLAJ1 (A5FIM3)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194265.1
- ProteinModelPortal:   A5FIM3
- SMR:   A5FIM3
- STRING:   A5FIM3
- GeneID:   5090441
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1914
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   SVKHTEE
- ProtClustDB:   PRK00062
- BioCyc:   FJOH376686:FJOH_1914-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45983; Mature: 45983

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYKRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGAYLYDEDGNKLIDYINS
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHC
WGPMVLGHAYQPVVDAVIEKAKLGTSFGMPTELETEIAALAVSMVPNIDKIRFVNSGTEA
CCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHH
CMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAVTFGSPNSPGVTEGTAKDTLL
HHHHHHHHHCCCHHHHEEEEEEEECCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHH
AKYNDLENVKTLIEANKGEIAAIIIEAVAGNMGCIPPAEGFLQGLRDLCTANGILLIFDE
HHHCCHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
VMTGFRLARGGVQELYGIDADIVTFGKVIGGGLPVGAFAAREEIMNYLAPLGPVYQAGTL
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCC
SGNPLAMAAGYAMLKALDNDREIFTRLEEKTAYLEAGIDRVLKANNVVFTINRVGSMISV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
HFDAGPVTDFKTAAKGDNETFKKFFHGLLQEGVYIAPSAYETWFITDALTYEDLDFTINA
EECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCEECCCCHHHHH
IDKVSKTF
HHHHHHCC
>Mature Secondary Structure
MLYKRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGAYLYDEDGNKLIDYINS
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHC
WGPMVLGHAYQPVVDAVIEKAKLGTSFGMPTELETEIAALAVSMVPNIDKIRFVNSGTEA
CCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHH
CMSAIRLARGFTKRDKIIKFAGCYHGHSDSFLIQAGSGAVTFGSPNSPGVTEGTAKDTLL
HHHHHHHHHCCCHHHHEEEEEEEECCCCCCEEEEECCCEEEECCCCCCCCCCCCCHHHHH
AKYNDLENVKTLIEANKGEIAAIIIEAVAGNMGCIPPAEGFLQGLRDLCTANGILLIFDE
HHHCCHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
VMTGFRLARGGVQELYGIDADIVTFGKVIGGGLPVGAFAAREEIMNYLAPLGPVYQAGTL
HHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCC
SGNPLAMAAGYAMLKALDNDREIFTRLEEKTAYLEAGIDRVLKANNVVFTINRVGSMISV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEE
HFDAGPVTDFKTAAKGDNETFKKFFHGLLQEGVYIAPSAYETWFITDALTYEDLDFTINA
EECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCEECCCCHHHHH
IDKVSKTF
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA