Click here to switch to the map view.

The map label for this gene is ppa [H]

Identifier: 146299238

GI number: 146299238

Start: 1693405

End: 1693935

Strand: Direct

Name: ppa [H]

Synonym: Fjoh_1478

Alternate gene names: 146299238

Gene position: 1693405-1693935 (Clockwise)

Preceding gene: 146299237

Following gene: 146299239

Centisome position: 27.77

GC content: 33.9

Gene sequence:

>531_bases
ATGACCGCAGACAAACTAACAACTTTCGATGTATTAATCGAAATACCAAGAGGAAGCAGAAATAAATATGAGTACGATTT
TGAAATCAAAAGAATGCGTTTCGACAGAATGTTATTCTCTTCAATGATGTACCCTGCAGATTACGGATTTATTCCAGAAA
CTTTAGCTCTTGACGGTGATCCTCTTGATGTATTGGTTTTAGTAAATGAACCAACTTTCCCTGGATGTGTTATGGAAGTA
AAACCAATTGGTGTTTTCCATATGGCAGATGATAAAGGACCAGACGAAAAAATTATTTGTGTACCAGTTTCAGATCCAAT
CTGGAATTCATTAAACGATCTTTCTGATATTAACCCTCACTTAGTAAAAGAAATTGAGCATTTCTTCCAGGTTTATAAAG
ATCTTGAAAACAAAAAAGTTGATGTTGAAGGATGGGGAGATGTAAAAGAGGCTTACGAAATTATTGCAGAGTGCACTAAG
CGTTTTGATGATATTGAAAATAAACCAGCAGGATTATTTAGTATTAAATAA

Upstream 100 bases:

>100_bases
TAATGTATATATATACAGATTAATATACTTTTTTCATTGTAAAAAAGGATTGTTTGGTTACTTTTGCAATCGAAATTATA
GATATAATAATAAAACAAAA

Downstream 100 bases:

>100_bases
TTTAAGCATTCATAATTTATAAAAAAAGCAATACTGCCGTCAGGAGTATTGCTTTTTTGTTTAAATTCGTTTTAGTTAGT
TTGTTGACTATTAACCAAAA

Product: inorganic diphosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MTADKLTTFDVLIEIPRGSRNKYEYDFEIKRMRFDRMLFSSMMYPADYGFIPETLALDGDPLDVLVLVNEPTFPGCVMEV
KPIGVFHMADDKGPDEKIICVPVSDPIWNSLNDLSDINPHLVKEIEHFFQVYKDLENKKVDVEGWGDVKEAYEIIAECTK
RFDDIENKPAGLFSIK

Sequences:

>Translated_176_residues
MTADKLTTFDVLIEIPRGSRNKYEYDFEIKRMRFDRMLFSSMMYPADYGFIPETLALDGDPLDVLVLVNEPTFPGCVMEV
KPIGVFHMADDKGPDEKIICVPVSDPIWNSLNDLSDINPHLVKEIEHFFQVYKDLENKKVDVEGWGDVKEAYEIIAECTK
RFDDIENKPAGLFSIK
>Mature_175_residues
TADKLTTFDVLIEIPRGSRNKYEYDFEIKRMRFDRMLFSSMMYPADYGFIPETLALDGDPLDVLVLVNEPTFPGCVMEVK
PIGVFHMADDKGPDEKIICVPVSDPIWNSLNDLSDINPHLVKEIEHFFQVYKDLENKKVDVEGWGDVKEAYEIIAECTKR
FDDIENKPAGLFSIK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=162, Percent_Identity=41.9753086419753, Blast_Score=115, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319483, Length=151, Percent_Identity=28.476821192053, Blast_Score=68, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221468704, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45549187, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 20245; Mature: 20114

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADKLTTFDVLIEIPRGSRNKYEYDFEIKRMRFDRMLFSSMMYPADYGFIPETLALDGD
CCCCHHEEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
PLDVLVLVNEPTFPGCVMEVKPIGVFHMADDKGPDEKIICVPVSDPIWNSLNDLSDINPH
CEEEEEEECCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCHH
LVKEIEHFFQVYKDLENKKVDVEGWGDVKEAYEIIAECTKRFDDIENKPAGLFSIK
HHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure 
TADKLTTFDVLIEIPRGSRNKYEYDFEIKRMRFDRMLFSSMMYPADYGFIPETLALDGD
CCCHHEEEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
PLDVLVLVNEPTFPGCVMEVKPIGVFHMADDKGPDEKIICVPVSDPIWNSLNDLSDINPH
CEEEEEEECCCCCCCEEEEEECCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCHH
LVKEIEHFFQVYKDLENKKVDVEGWGDVKEAYEIIAECTKRFDDIENKPAGLFSIK
HHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]