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The map label for this gene is pyk [H]

Identifier: 146298902

GI number: 146298902

Start: 1287778

End: 1289211

Strand: Direct

Name: pyk [H]

Synonym: Fjoh_1141

Alternate gene names: 146298902

Gene position: 1287778-1289211 (Clockwise)

Preceding gene: 146298901

Following gene: 146298903

Centisome position: 21.12

GC content: 34.59

Gene sequence:

>1434_bases
ATGTTAACAAATAAGAAAACCAAAATTGTTGCTACACTTGGGCCTGCCTGTAGTACAAGAGAGATCATTAAAGATATGAT
TGATGCAGGGGTTAATGTGTTTAGAATCAATTTTTCGCATGCAGATTACGAAGGAGTAAAAGAAAAAATTGACATCATTA
GAGGATTAAACGAAGAGTTTGGTTATACTACAGCGATTCTTGGAGATTTGCAGGGACCAAAACTTAGAGTTGGTGTAATG
GAAGAAGGTACTGTTGTACATGATGGAGATGAAATTACTTTTACAACTGCAGAAGATATTATCGGAAATGCAAAAAAAGC
ATTCATGAAATATCAGAATTTCCCAAATGATGTAAATGTTGGAGAGCGTATTTTGCTTGATGATGGTAAACTTATTTTCG
AAATTGTTTCAACAGATAAAAAAACAGAAGTTGTTGCTAAAGTTATTCAGGGCGGAGAATTAAAATCTAAAAAAGGAGTT
AATCTTCCAAATACAAAAATTTCTTTACCAGCTTTAACTGAAAAAGATATTGCTGATGCCATTTTTGCAATCGAACAAAA
AGTAGACTGGATCGCACTTTCATTCGTGAAAACACCGCGCGATTTACAAGATTTACAAGAATTAATCGCGAAGCATTCAG
AATATAAAATTCCAATTATTGCTAAAATTGAAATGCCGGAAGCTCTAGAGAACATGGATAAAATTGTAGCATATTGCGAT
GGTTTAATGGTGGCTCGTGGAGATCTTGGTGTTGAGCTTCCTGCTCACGAAGTTCCATTAGTACAAAAAGATTTGATCAG
AAGAGCTAAAACAGCTAGAATTCCTGTTATCGTTGCTACACAAATGATGGAAACAATGATTACAAGTTTAACTCCAACAA
GAGCAGAAGTAAACGACGTTGCAAACTCAGTAATGGACGGAGCAGATGCAGTAATGTTGTCTGGAGAAACTGCAACAGGA
AACTATCCAGTACAAGTAATTCAAAGAATGGCACAAATTTGCGAAGCTGTAGAAAACTCTCCGCTTATTCAAGTGCCTCA
AAATACACCACAAATTAAAACTAAACGTTTTGTTACTAAAACAGTTTGCCACCAGGCAGCTTTATTAGCAAATGAAATCG
AAGCTAAAGCAATTTGTACTTTAACAAACAGTGGTTACACAGCTTTCCAAATTTCGGCTTGGAGACCTTCAACGGCTCAT
ATTTTAGTATTTACTTCAAACAGAAGAATCTTAACACAATTGAATTTATTATGGGGAGTTAAATCTTTCTACTATGATAA
TGAAGAAAGTACAGATGATACTGTAACTGATGTAAATCAAATTGCAATTGAAAAAGGTTATGCACAAAAAGGAGATTATT
TAATCAACCTTGCTGCAATGCCAATTAAAGATAAAGGAATGGTTAACACCATGAGAGTTTCTGAGATAGAATAG

Upstream 100 bases:

>100_bases
AAATTCAGCAAAAACTAAATTCAATAGAAAGTATCGCAGCAATATATGCTGTAGATACCAATAAAATAAAATCAAAAAAC
AATCTAATTTTTTAAAAAAA

Downstream 100 bases:

>100_bases
TTTCTGTAAAAGATCCTAAATATACTTTTAAAAAGCCATCCGGTAAATCGGGTGGCTTTTTTGCTTTTATACCCTGAGTG
AAAATTGTTTCAGTTTAAAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYEGVKEKIDIIRGLNEEFGYTTAILGDLQGPKLRVGVM
EEGTVVHDGDEITFTTAEDIIGNAKKAFMKYQNFPNDVNVGERILLDDGKLIFEIVSTDKKTEVVAKVIQGGELKSKKGV
NLPNTKISLPALTEKDIADAIFAIEQKVDWIALSFVKTPRDLQDLQELIAKHSEYKIPIIAKIEMPEALENMDKIVAYCD
GLMVARGDLGVELPAHEVPLVQKDLIRRAKTARIPVIVATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETATG
NYPVQVIQRMAQICEAVENSPLIQVPQNTPQIKTKRFVTKTVCHQAALLANEIEAKAICTLTNSGYTAFQISAWRPSTAH
ILVFTSNRRILTQLNLLWGVKSFYYDNEESTDDTVTDVNQIAIEKGYAQKGDYLINLAAMPIKDKGMVNTMRVSEIE

Sequences:

>Translated_477_residues
MLTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYEGVKEKIDIIRGLNEEFGYTTAILGDLQGPKLRVGVM
EEGTVVHDGDEITFTTAEDIIGNAKKAFMKYQNFPNDVNVGERILLDDGKLIFEIVSTDKKTEVVAKVIQGGELKSKKGV
NLPNTKISLPALTEKDIADAIFAIEQKVDWIALSFVKTPRDLQDLQELIAKHSEYKIPIIAKIEMPEALENMDKIVAYCD
GLMVARGDLGVELPAHEVPLVQKDLIRRAKTARIPVIVATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETATG
NYPVQVIQRMAQICEAVENSPLIQVPQNTPQIKTKRFVTKTVCHQAALLANEIEAKAICTLTNSGYTAFQISAWRPSTAH
ILVFTSNRRILTQLNLLWGVKSFYYDNEESTDDTVTDVNQIAIEKGYAQKGDYLINLAAMPIKDKGMVNTMRVSEIE
>Mature_477_residues
MLTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYEGVKEKIDIIRGLNEEFGYTTAILGDLQGPKLRVGVM
EEGTVVHDGDEITFTTAEDIIGNAKKAFMKYQNFPNDVNVGERILLDDGKLIFEIVSTDKKTEVVAKVIQGGELKSKKGV
NLPNTKISLPALTEKDIADAIFAIEQKVDWIALSFVKTPRDLQDLQELIAKHSEYKIPIIAKIEMPEALENMDKIVAYCD
GLMVARGDLGVELPAHEVPLVQKDLIRRAKTARIPVIVATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETATG
NYPVQVIQRMAQICEAVENSPLIQVPQNTPQIKTKRFVTKTVCHQAALLANEIEAKAICTLTNSGYTAFQISAWRPSTAH
ILVFTSNRRILTQLNLLWGVKSFYYDNEESTDDTVTDVNQIAIEKGYAQKGDYLINLAAMPIKDKGMVNTMRVSEIE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=37.5757575757576, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=37.1717171717172, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=36.8421052631579, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=36.0557768924303, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=36.0557768924303, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=37.6712328767123, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=37.6712328767123, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI310128736, Length=240, Percent_Identity=35.4166666666667, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128734, Length=240, Percent_Identity=35.4166666666667, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI310128738, Length=221, Percent_Identity=33.9366515837104, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=40.552016985138, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1788160, Length=485, Percent_Identity=38.1443298969072, Blast_Score=279, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=37.603305785124, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=37.7551020408163, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=37.7551020408163, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=37.7551020408163, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=37.7551020408163, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=494, Percent_Identity=37.246963562753, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=33.9468302658487, Blast_Score=247, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=35.9756097560976, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=35.9756097560976, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.5609756097561, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=31.055900621118, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24646914, Length=172, Percent_Identity=48.2558139534884, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52722; Mature: 52722

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYEGVKEKIDIIRGLNEEF
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHH
GYTTAILGDLQGPKLRVGVMEEGTVVHDGDEITFTTAEDIIGNAKKAFMKYQNFPNDVNV
CEEEEEEECCCCCEEEEEEECCCCEEECCCEEEEEEHHHHHCHHHHHHHHHHCCCCCCCC
GERILLDDGKLIFEIVSTDKKTEVVAKVIQGGELKSKKGVNLPNTKISLPALTEKDIADA
CCEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
IFAIEQKVDWIALSFVKTPRDLQDLQELIAKHSEYKIPIIAKIEMPEALENMDKIVAYCD
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
GLMVARGDLGVELPAHEVPLVQKDLIRRAKTARIPVIVATQMMETMITSLTPTRAEVNDV
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHH
ANSVMDGADAVMLSGETATGNYPVQVIQRMAQICEAVENSPLIQVPQNTPQIKTKRFVTK
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
TVCHQAALLANEIEAKAICTLTNSGYTAFQISAWRPSTAHILVFTSNRRILTQLNLLWGV
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEEHHHHHHHHH
KSFYYDNEESTDDTVTDVNQIAIEKGYAQKGDYLINLAAMPIKDKGMVNTMRVSEIE
HHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MLTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYEGVKEKIDIIRGLNEEF
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHH
GYTTAILGDLQGPKLRVGVMEEGTVVHDGDEITFTTAEDIIGNAKKAFMKYQNFPNDVNV
CEEEEEEECCCCCEEEEEEECCCCEEECCCEEEEEEHHHHHCHHHHHHHHHHCCCCCCCC
GERILLDDGKLIFEIVSTDKKTEVVAKVIQGGELKSKKGVNLPNTKISLPALTEKDIADA
CCEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
IFAIEQKVDWIALSFVKTPRDLQDLQELIAKHSEYKIPIIAKIEMPEALENMDKIVAYCD
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHC
GLMVARGDLGVELPAHEVPLVQKDLIRRAKTARIPVIVATQMMETMITSLTPTRAEVNDV
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCHHHHHHH
ANSVMDGADAVMLSGETATGNYPVQVIQRMAQICEAVENSPLIQVPQNTPQIKTKRFVTK
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
TVCHQAALLANEIEAKAICTLTNSGYTAFQISAWRPSTAHILVFTSNRRILTQLNLLWGV
HHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEEHHHHHHHHH
KSFYYDNEESTDDTVTDVNQIAIEKGYAQKGDYLINLAAMPIKDKGMVNTMRVSEIE
HHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]