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The map label for this gene is rutD

Identifier: 146283917

GI number: 146283917

Start: 3887957

End: 3888754

Strand: Direct

Name: rutD

Synonym: PST_3600

Alternate gene names: 146283917

Gene position: 3887957-3888754 (Clockwise)

Preceding gene: 146283916

Following gene: 146283918

Centisome position: 85.12

GC content: 65.54

Gene sequence:

>798_bases
ATGCACTACGAACTTCATGGTCGCATGGAGCCGGATGCACCGACCCTGGTGCTCAGTTCCGGCCTCGGCGGCGCCGCTGC
CTTCTGGTTGCCGCAGCTGCCGGCACTGACGCAGGACTACCGGGTGCTGGTCTACGATCAGCTGGGGACCAACAAGAGCC
CGGCGAACCTGCCGGCGGGCTACTCCATCGAATCCATGGCAGTGGAATTACTGGAGCTGCTGGACACCCTGGGCATCCGC
CGCTGCCATTTCATCGGCCATGCACTCGGCGGGCTGGTCGGCTTGCAGATCGCCCTGCTGCGGCCGCAGCTGTTGCAGAG
CCTGGTGCCGATCAATGCCTGGAGCAGCCCCAACCCGCACAGTGCGCGCTGTTTCGCAGTACGCCTGAAGCTGTTGCACG
ACAGCGGCCCGGCAGCCTATGTGCAGGCGCAGTCGCTGTTCCTCTACCCGGCCGACTGGATCGCGGCCAACAGCGAGCGC
CTGGCACGCGACGATGCCCATGCACTGGCGCATTTCCCACCGACGATGAATCTGGTGCGGCGCATCGAGGCACTGCTGGC
CTTCGATATCGAGGCCGAGCTGCCGCGCATCACCACCCCGACCCTGCTGATCGCCAACCGCGACGACATGCTGGTGCCCT
GGCAACGCTCGCAGCACCTGGCCGACAACATGCCCAACGCACAGCTGGCGCTGCTGAACTACGGTGGCCACGCGTCCAGC
GTCAGCGACAGCGAACCCTTCAACCAGATCCTGCTGGACCATCTTGCCGAGCAATGCGGCCAGGTCGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
TCGAGATCGCCAGCATCGCCCACGTCGGCCAATAGCGCCTACCAGAGCGGGTCAGCCGGCACGCCGGCGGCCCGCTGGTC
GACCTCGCGGAGGGAGTGAC

Downstream 100 bases:

>100_bases
GGACCCGAACATGCACGCCCCCATCGACTCGAATGCCCTCGCGACGCTGTTCAGCGAGGCCCGCACCCACAGCGCCTGGT
TGGACAAAGCGGTACCGGAT

Product: alpha/beta fold family hydrolase

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MHYELHGRMEPDAPTLVLSSGLGGAAAFWLPQLPALTQDYRVLVYDQLGTNKSPANLPAGYSIESMAVELLELLDTLGIR
RCHFIGHALGGLVGLQIALLRPQLLQSLVPINAWSSPNPHSARCFAVRLKLLHDSGPAAYVQAQSLFLYPADWIAANSER
LARDDAHALAHFPPTMNLVRRIEALLAFDIEAELPRITTPTLLIANRDDMLVPWQRSQHLADNMPNAQLALLNYGGHASS
VSDSEPFNQILLDHLAEQCGQVAAA

Sequences:

>Translated_265_residues
MHYELHGRMEPDAPTLVLSSGLGGAAAFWLPQLPALTQDYRVLVYDQLGTNKSPANLPAGYSIESMAVELLELLDTLGIR
RCHFIGHALGGLVGLQIALLRPQLLQSLVPINAWSSPNPHSARCFAVRLKLLHDSGPAAYVQAQSLFLYPADWIAANSER
LARDDAHALAHFPPTMNLVRRIEALLAFDIEAELPRITTPTLLIANRDDMLVPWQRSQHLADNMPNAQLALLNYGGHASS
VSDSEPFNQILLDHLAEQCGQVAAA
>Mature_265_residues
MHYELHGRMEPDAPTLVLSSGLGGAAAFWLPQLPALTQDYRVLVYDQLGTNKSPANLPAGYSIESMAVELLELLDTLGIR
RCHFIGHALGGLVGLQIALLRPQLLQSLVPINAWSSPNPHSARCFAVRLKLLHDSGPAAYVQAQSLFLYPADWIAANSER
LARDDAHALAHFPPTMNLVRRIEALLAFDIEAELPRITTPTLLIANRDDMLVPWQRSQHLADNMPNAQLALLNYGGHASS
VSDSEPFNQILLDHLAEQCGQVAAA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=237, Percent_Identity=48.1012658227848, Blast_Score=220, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_PSEU5 (A4VQH7)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174070.1
- ProteinModelPortal:   A4VQH7
- STRING:   A4VQH7
- GeneID:   5096561
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3600
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   YPADWIA
- PhylomeDB:   A4VQH7
- ProtClustDB:   CLSK912658
- BioCyc:   PSTU379731:PST_3600-MONOMER
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- PRINTS:   PR00111
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 28956; Mature: 28956

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYELHGRMEPDAPTLVLSSGLGGAAAFWLPQLPALTQDYRVLVYDQLGTNKSPANLPAG
CCEEECCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
YSIESMAVELLELLDTLGIRRCHFIGHALGGLVGLQIALLRPQLLQSLVPINAWSSPNPH
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SARCFAVRLKLLHDSGPAAYVQAQSLFLYPADWIAANSERLARDDAHALAHFPPTMNLVR
CHHHHHEEEHHHHCCCCEEEEEEEEEEEECHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
RIEALLAFDIEAELPRITTPTLLIANRDDMLVPWQRSQHLADNMPNAQLALLNYGGHASS
HHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCEEEEEECCCCCCC
VSDSEPFNQILLDHLAEQCGQVAAA
CCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHYELHGRMEPDAPTLVLSSGLGGAAAFWLPQLPALTQDYRVLVYDQLGTNKSPANLPAG
CCEEECCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
YSIESMAVELLELLDTLGIRRCHFIGHALGGLVGLQIALLRPQLLQSLVPINAWSSPNPH
CCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SARCFAVRLKLLHDSGPAAYVQAQSLFLYPADWIAANSERLARDDAHALAHFPPTMNLVR
CHHHHHEEEHHHHCCCCEEEEEEEEEEEECHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
RIEALLAFDIEAELPRITTPTLLIANRDDMLVPWQRSQHLADNMPNAQLALLNYGGHASS
HHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCCEEEEEECCCCCCC
VSDSEPFNQILLDHLAEQCGQVAAA
CCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA