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The map label for this gene is entB [C]

Identifier: 145301201

GI number: 145301201

Start: 4694346

End: 4694597

Strand: Direct

Name: entB [C]

Synonym: ASA_4379

Alternate gene names: 145301201

Gene position: 4694346-4694597 (Clockwise)

Preceding gene: 145301198

Following gene: 145301202

Centisome position: 99.83

GC content: 43.25

Gene sequence:

>252_bases
ATGACCAATCCAGAAATGATTAGCCATCAATGGCTACAAGAGCGCGTACAGCAACTTATTGAAGACAATGACACCGAGCT
GGATCTGGAAGAGAGCCTTATTTTATATGGGCTAGACTCTTTGCGAGTGATGCGATTTGCTGCTGAACTTAAAGAACGCA
ACATCCATGTCAGTTTTGAAGATCTGATAAAATCACCAACATTGGCTAGCTGGCGGGAGTTGATTGCCAATCGACAAGCC
ATTGCAAGATAA

Upstream 100 bases:

>100_bases
CCCTGCTCAATGTGTGGAGGCGTTAGGTCCATAGTTATGATGTGGGTATTTGTGTCTGCTGTTATTTACAATGACATCAA
CAATATGAGGCTGGTTTCAC

Downstream 100 bases:

>100_bases
ACGTAAATTATAGTTTTGACAGCAAGAGTCCGCGTGATGGCTTTTTACTTTTATTAATGGTTGTTTCACCGCCATCCCTT
ACGATTAATCATCGGCAACT

Product: isochorismatase

Products: 2,3-dihydroxy-2,3-dihydrobenzoate; pyruvate

Alternate protein names: Enterobactin Isochorismatase; Isochorismate Hydrolase; 2 3-Dihydroxybenzoate-AMP Ligase

Number of amino acids: Translated: 83; Mature: 82

Protein sequence:

>83_residues
MTNPEMISHQWLQERVQQLIEDNDTELDLEESLILYGLDSLRVMRFAAELKERNIHVSFEDLIKSPTLASWRELIANRQA
IAR

Sequences:

>Translated_83_residues
MTNPEMISHQWLQERVQQLIEDNDTELDLEESLILYGLDSLRVMRFAAELKERNIHVSFEDLIKSPTLASWRELIANRQA
IAR
>Mature_82_residues
TNPEMISHQWLQERVQQLIEDNDTELDLEESLILYGLDSLRVMRFAAELKERNIHVSFEDLIKSPTLASWRELIANRQAI
AR

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG3433

COG function: function code Q; Aryl carrier domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.3.2.1

Molecular weight: Translated: 9780; Mature: 9649

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPEMISHQWLQERVQQLIEDNDTELDLEESLILYGLDSLRVMRFAAELKERNIHVSFE
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLIKSPTLASWRELIANRQAIAR
HHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNPEMISHQWLQERVQQLIEDNDTELDLEESLILYGLDSLRVMRFAAELKERNIHVSFE
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLIKSPTLASWRELIANRQAIAR
HHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; isochorismate

Specific reaction: H2O + isochorismate = 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA