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The map label for this gene is napF [C]

Identifier: 145300993

GI number: 145300993

Start: 4438153

End: 4438671

Strand: Direct

Name: napF [C]

Synonym: ASA_4147

Alternate gene names: 145300993

Gene position: 4438153-4438671 (Clockwise)

Preceding gene: 145300990

Following gene: 145301000

Centisome position: 94.38

GC content: 63.58

Gene sequence:

>519_bases
GTGAGCATGACTCATTTTTTCTGTCGATGGCTGACGCACGCACTGGGGGGCACCCGCAAACCGGACGTGGTCTGTCACGA
GGCTCGCCCGCCATGGGCCATCGAAGAGTCCCAGTACCTCTCCCTCTGCACCCGTTGCGGTGAGTGCTTCAAGGCCTGCC
CCAACGGCCTGCTCAAACCGGCCAGTCAACCCGAATACGAAGGTACCGCCATCGCCGGCACCCCTGTGCTGGATCTCGCC
TGCGGCCAGTGCAGCTATTGTGGCAGCTGTGCCAGAGCCTGCCCGACCGGCGCTCTCGATCTGCAATGGGGGCGCCAGGT
GCAGACCCGGGTTCAGATTGAAGCGAGCTGTCAGGCACGACAGGGCTTCTACTGCCTGCTGTGTGAAGATGCCTGTCCCC
AGCAGGCGATCAAGGCTACCAGCGATGGGGTGAGCGTCAACATGGCGGCGTGTGATGGCTGCGGTGCCTGCGGTCTCGCC
TGCTTGCACGGCGCCATCACCCTGATCCCACAGCTTTGA

Upstream 100 bases:

>100_bases
AGCTTACACAAGGGCAACCCGGCTTGCGATCCTGACCGCCGCTATTCTCTGGCTTGCCAGCAGAAACTTGGATAAAATTA
ACCCCTAATAGGTATCGGGG

Downstream 100 bases:

>100_bases
CCCCCAGAACCCCGTCTTCAGCACTGAAGCCATATTCCACTGCTACCAGACGCCAGAAAACACAAAGGGGCCCGTGGGCC
CCTCATGGTCTGACGCTGGC

Product: ferredoxin 2

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSMTHFFCRWLTHALGGTRKPDVVCHEARPPWAIEESQYLSLCTRCGECFKACPNGLLKPASQPEYEGTAIAGTPVLDLA
CGQCSYCGSCARACPTGALDLQWGRQVQTRVQIEASCQARQGFYCLLCEDACPQQAIKATSDGVSVNMAACDGCGACGLA
CLHGAITLIPQL

Sequences:

>Translated_172_residues
MSMTHFFCRWLTHALGGTRKPDVVCHEARPPWAIEESQYLSLCTRCGECFKACPNGLLKPASQPEYEGTAIAGTPVLDLA
CGQCSYCGSCARACPTGALDLQWGRQVQTRVQIEASCQARQGFYCLLCEDACPQQAIKATSDGVSVNMAACDGCGACGLA
CLHGAITLIPQL
>Mature_171_residues
SMTHFFCRWLTHALGGTRKPDVVCHEARPPWAIEESQYLSLCTRCGECFKACPNGLLKPASQPEYEGTAIAGTPVLDLAC
GQCSYCGSCARACPTGALDLQWGRQVQTRVQIEASCQARQGFYCLLCEDACPQQAIKATSDGVSVNMAACDGCGACGLAC
LHGAITLIPQL

Specific function: Involved In Electron Transfer. [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=144, Percent_Identity=34.7222222222222, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788533, Length=165, Percent_Identity=33.9393939393939, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18326; Mature: 18195

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
12.8 %Cys+Met (Translated Protein)
11.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTHFFCRWLTHALGGTRKPDVVCHEARPPWAIEESQYLSLCTRCGECFKACPNGLLKP
CCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
ASQPEYEGTAIAGTPVLDLACGQCSYCGSCARACPTGALDLQWGRQVQTRVQIEASCQAR
CCCCCCCCEEECCCCHHECCCCCCHHHHHHHHHCCCCCEECCCCCCCHHHEEEEECCCCC
QGFYCLLCEDACPQQAIKATSDGVSVNMAACDGCGACGLACLHGAITLIPQL
CCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMTHFFCRWLTHALGGTRKPDVVCHEARPPWAIEESQYLSLCTRCGECFKACPNGLLKP
CHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
ASQPEYEGTAIAGTPVLDLACGQCSYCGSCARACPTGALDLQWGRQVQTRVQIEASCQAR
CCCCCCCCEEECCCCHHECCCCCCHHHHHHHHHCCCCCEECCCCCCCHHHEEEEECCCCC
QGFYCLLCEDACPQQAIKATSDGVSVNMAACDGCGACGLACLHGAITLIPQL
CCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]