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The map label for this gene is puuR [H]

Identifier: 145301000

GI number: 145301000

Start: 4445541

End: 4446083

Strand: Direct

Name: puuR [H]

Synonym: ASA_4154

Alternate gene names: 145301000

Gene position: 4445541-4446083 (Clockwise)

Preceding gene: 145300993

Following gene: 145301001

Centisome position: 94.54

GC content: 61.33

Gene sequence:

>543_bases
ATGGATATCGGTCACCGCCTCAAGGCGGTTCGCACCAAGGCGGCCCTCTCCCAGCGCGAGCTGGCCAAACGCTCCGGTGT
GACCAACGGTTTTATCTCCCAGATCGAGAAAAATCAGGTGAGCCCCTCAGTGTCCTCACTGCGCAAGGTACTCGAAGGCA
TTCCCATGTCCCTGGCCAGCTTCTTCACCGAAGAGACAGAGATGGGGACAGAGGTAATCTTCCGCGCCAGCGACATGCCG
GATCTCGGCACCCATCCCATCAGCTACCGACTGGTGGGCCACAGCCGCGCCAACCGTGCCATCGGCATGATGCAGGAGAT
ACTCCCGCCAGGCGCAGACACGGGTGACGACATGCTGAGCCACGAAGGGGAAGAGTGCGGCATCGTCATTCAGGGGACGG
TCGAGGTGACCATTGGCGAGCAGATCCACCAGCTGGCACCGGGTGATGGCTACTACTTCGACAGCCGCACGCCGCACCGT
TTTCGCAACGTGGGCGAGCAGGAGTGCGTGCTGATCTCTGCCAACACGCCGGCCAGTTTCTAA

Upstream 100 bases:

>100_bases
AAGGGCTCAATGTTGCCCTGCTTGGTGTCACCCCGACCGGGGTGGTATAAGATATTTAACACAATTTTAATAATAGTAAA
CAGTCAAGGAGTGTCTATTG

Downstream 100 bases:

>100_bases
GCCGCCATTTGACAGGGTTCGGACTGATCTCGGGCAAGTCACGCCGGCCAGTTTCCAAATCGCCATTTGACAGGGTTCGG
GCTGATCTCGAAGGCCACAC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MDIGHRLKAVRTKAALSQRELAKRSGVTNGFISQIEKNQVSPSVSSLRKVLEGIPMSLASFFTEETEMGTEVIFRASDMP
DLGTHPISYRLVGHSRANRAIGMMQEILPPGADTGDDMLSHEGEECGIVIQGTVEVTIGEQIHQLAPGDGYYFDSRTPHR
FRNVGEQECVLISANTPASF

Sequences:

>Translated_180_residues
MDIGHRLKAVRTKAALSQRELAKRSGVTNGFISQIEKNQVSPSVSSLRKVLEGIPMSLASFFTEETEMGTEVIFRASDMP
DLGTHPISYRLVGHSRANRAIGMMQEILPPGADTGDDMLSHEGEECGIVIQGTVEVTIGEQIHQLAPGDGYYFDSRTPHR
FRNVGEQECVLISANTPASF
>Mature_180_residues
MDIGHRLKAVRTKAALSQRELAKRSGVTNGFISQIEKNQVSPSVSSLRKVLEGIPMSLASFFTEETEMGTEVIFRASDMP
DLGTHPISYRLVGHSRANRAIGMMQEILPPGADTGDDMLSHEGEECGIVIQGTVEVTIGEQIHQLAPGDGYYFDSRTPHR
FRNVGEQECVLISANTPASF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=178, Percent_Identity=37.6404494382022, Blast_Score=126, Evalue=8e-31,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 19675; Mature: 19675

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIGHRLKAVRTKAALSQRELAKRSGVTNGFISQIEKNQVSPSVSSLRKVLEGIPMSLAS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
FFTEETEMGTEVIFRASDMPDLGTHPISYRLVGHSRANRAIGMMQEILPPGADTGDDMLS
HHHHHHHCCHHHEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHH
HEGEECGIVIQGTVEVTIGEQIHQLAPGDGYYFDSRTPHRFRNVGEQECVLISANTPASF
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCEEECCCCCHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MDIGHRLKAVRTKAALSQRELAKRSGVTNGFISQIEKNQVSPSVSSLRKVLEGIPMSLAS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHH
FFTEETEMGTEVIFRASDMPDLGTHPISYRLVGHSRANRAIGMMQEILPPGADTGDDMLS
HHHHHHHCCHHHEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHH
HEGEECGIVIQGTVEVTIGEQIHQLAPGDGYYFDSRTPHRFRNVGEQECVLISANTPASF
CCCCCCCEEEEEEEEEEEHHHHHHHCCCCCEEECCCCCHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]