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The map label for this gene is brnQ [H]

Identifier: 145300505

GI number: 145300505

Start: 3926733

End: 3928040

Strand: Direct

Name: brnQ [H]

Synonym: ASA_3637

Alternate gene names: 145300505

Gene position: 3926733-3928040 (Clockwise)

Preceding gene: 145300504

Following gene: 145300509

Centisome position: 83.5

GC content: 58.03

Gene sequence:

>1308_bases
TTGACGAAATCACTCACATTATCCGATGTACTCGGATTGGGCTTCATGACCTTTGCGTTCTATCTGGGCGCCGGTAACAT
CATTTTCCCGCCATTGGCAGGCTACATGGCAGGTGAGCACCTCTCCCTGGCCATGCTGGGCTTCCTGGTCACAGCCGTGG
GCCTGCCCCTCATCACTATCATTGCTGTCGCCAAAGCGGGCAATGGCTGGGCTGGCATGACAAAATTTCTGCCTGCCGGA
CTGGCAACCGCACTGGCCGTGGCCATCTATATCATCATTGGCCCAGCCTTTGCTGCTCCCCGTACCGGTCTGGTTGCCTA
TGAAATGGGCCTCAAGCCCTTTATCGGTGATATGGGGCAAGCCGGTCTTGCCGCCTATACAGTGATCTTCTTCGGCATTG
CCATACTGGTCTCCCTCAATCAGGGCAAGCTGATGGATACCATCGGCAAATACCTGATGCCGGTTCTGATGGTACTGTTG
CTAACCCTGGCCGTCGGCGTCTTCGTCGCACCGCAAGGCACAATACCGAGCGCCACCGGTGACTATCTGGAGGGCCCGTT
CGTGAAGGGGATCCTGGAAGGCTATAACACCATGGATACCCTGGCCTCCCTGATGTTTGGTGCCCTGATCATCGATCTGC
TGCGCCAGAAGGGGATCAACGACTACAAGAGCCAGTTCAAATATCTGGCCATTGCAGGCCTTATATCTGCGATCGGTCTG
TCCGTGGTCTATGTTTCCCTGTTCCAGCTGGGTAATACCGCAGCCGGGGTTGCCACCGACGTCAGCAATGGCGGCGCCAT
CGTCAACGCCTATGTGCTGAGCCTGTTCGGTCAACCGGGTCAGTTCATTCTGGCCGGCATCATCACCCTGGCTTGCTTCA
CTACCGCAGTGGGCCTCATCTCCGCCTGCTCCGACTTCTTCCACAATCTCACAGGCATGAGCTACAAGAAGCTAGTTGTC
CTGCTGGGTACCATCTGCGCCATCGTCGCCAACGTGGGTCTGAGCCAGCTTATCAGCCTCTCCATCCCTGTGCTGGTCGC
CATCTATCCGGTCGCAGTGGCACTGGTGCTGGTCACCTTCCTGAAGGGATACTTCGGTCGTCCCCGTCTGGCATTCCGCT
CTGTCCTGATGGTAGCGTTCCTGTTCGGTTGCCTGGATGGTCTGGGTGCTGCCGGCATGAAGATGGATGCCTTTGCCTTC
CTGCCACTGTTTGACAAGGGCCTGGCCTGGTTGCTGCCGACCCTGCTGGCCTGTGGCCTGGGCATGGCGGTGCGCTCCAG
CGAGAAGCTGGCTGCCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
AAATACGCGGGGCTCCTTTTTTTTACCCCTGAATAACGCCGGTCACTGACCAATAAAAAACTATAACAAGCAATAGTTTA
CAAGACAGACGAGGTTGAAC

Downstream 100 bases:

>100_bases
CAACAATTTGTTCCGATATAAAAAGAGGCCAGCATATGCTGGCCTCTCTCTTTTCTCTGATCCGTCACCACAACATCAAA
GCAGCGGGACTATCGGTTTG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTKSLTLSDVLGLGFMTFAFYLGAGNIIFPPLAGYMAGEHLSLAMLGFLVTAVGLPLITIIAVAKAGNGWAGMTKFLPAG
LATALAVAIYIIIGPAFAAPRTGLVAYEMGLKPFIGDMGQAGLAAYTVIFFGIAILVSLNQGKLMDTIGKYLMPVLMVLL
LTLAVGVFVAPQGTIPSATGDYLEGPFVKGILEGYNTMDTLASLMFGALIIDLLRQKGINDYKSQFKYLAIAGLISAIGL
SVVYVSLFQLGNTAAGVATDVSNGGAIVNAYVLSLFGQPGQFILAGIITLACFTTAVGLISACSDFFHNLTGMSYKKLVV
LLGTICAIVANVGLSQLISLSIPVLVAIYPVAVALVLVTFLKGYFGRPRLAFRSVLMVAFLFGCLDGLGAAGMKMDAFAF
LPLFDKGLAWLLPTLLACGLGMAVRSSEKLAAEAA

Sequences:

>Translated_435_residues
MTKSLTLSDVLGLGFMTFAFYLGAGNIIFPPLAGYMAGEHLSLAMLGFLVTAVGLPLITIIAVAKAGNGWAGMTKFLPAG
LATALAVAIYIIIGPAFAAPRTGLVAYEMGLKPFIGDMGQAGLAAYTVIFFGIAILVSLNQGKLMDTIGKYLMPVLMVLL
LTLAVGVFVAPQGTIPSATGDYLEGPFVKGILEGYNTMDTLASLMFGALIIDLLRQKGINDYKSQFKYLAIAGLISAIGL
SVVYVSLFQLGNTAAGVATDVSNGGAIVNAYVLSLFGQPGQFILAGIITLACFTTAVGLISACSDFFHNLTGMSYKKLVV
LLGTICAIVANVGLSQLISLSIPVLVAIYPVAVALVLVTFLKGYFGRPRLAFRSVLMVAFLFGCLDGLGAAGMKMDAFAF
LPLFDKGLAWLLPTLLACGLGMAVRSSEKLAAEAA
>Mature_434_residues
TKSLTLSDVLGLGFMTFAFYLGAGNIIFPPLAGYMAGEHLSLAMLGFLVTAVGLPLITIIAVAKAGNGWAGMTKFLPAGL
ATALAVAIYIIIGPAFAAPRTGLVAYEMGLKPFIGDMGQAGLAAYTVIFFGIAILVSLNQGKLMDTIGKYLMPVLMVLLL
TLAVGVFVAPQGTIPSATGDYLEGPFVKGILEGYNTMDTLASLMFGALIIDLLRQKGINDYKSQFKYLAIAGLISAIGLS
VVYVSLFQLGNTAAGVATDVSNGGAIVNAYVLSLFGQPGQFILAGIITLACFTTAVGLISACSDFFHNLTGMSYKKLVVL
LGTICAIVANVGLSQLISLSIPVLVAIYPVAVALVLVTFLKGYFGRPRLAFRSVLMVAFLFGCLDGLGAAGMKMDAFAFL
PLFDKGLAWLLPTLLACGLGMAVRSSEKLAAEAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=419, Percent_Identity=42.7207637231504, Blast_Score=343, Evalue=1e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45255; Mature: 45124

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHCCCHHHHHCCC
>Mature Secondary Structure 
TKSLTLSDVLGLGFMTFAFYLGAGNIIFPPLAGYMAGEHLSLAMLGFLVTAVGLPLITI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IAVAKAGNGWAGMTKFLPAGLATALAVAIYIIIGPAFAAPRTGLVAYEMGLKPFIGDMGQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHCCCCHHHHCCCC
AGLAAYTVIFFGIAILVSLNQGKLMDTIGKYLMPVLMVLLLTLAVGVFVAPQGTIPSATG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DYLEGPFVKGILEGYNTMDTLASLMFGALIIDLLRQKGINDYKSQFKYLAIAGLISAIGL
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SVVYVSLFQLGNTAAGVATDVSNGGAIVNAYVLSLFGQPGQFILAGIITLACFTTAVGLI
HHHHHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SACSDFFHNLTGMSYKKLVVLLGTICAIVANVGLSQLISLSIPVLVAIYPVAVALVLVTF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGYFGRPRLAFRSVLMVAFLFGCLDGLGAAGMKMDAFAFLPLFDKGLAWLLPTLLACGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
GMAVRSSEKLAAEAA
CHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]