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The map label for this gene is dusA [H]

Identifier: 145300509

GI number: 145300509

Start: 3929390

End: 3930385

Strand: Direct

Name: dusA [H]

Synonym: ASA_3641

Alternate gene names: 145300509

Gene position: 3929390-3930385 (Clockwise)

Preceding gene: 145300505

Following gene: 145300510

Centisome position: 83.56

GC content: 60.14

Gene sequence:

>996_bases
ATGATTTTTATGCAGGCGCAGCGTTTTTCCATCGCCCCCATGCTGGACTGGACCGATCGGCATTGCCGTTATTTCCACCG
TCTGATGACCCGCCAGACCTTGCTTTATACCGAAATGGTGACCACGGGTGCCATCATCCATGGCAAAGGAGATTATCTGG
GTTACAGCGAGCAGGAGCGCCCCGTCTCCCTGCAACTGGGTGGCAGCAATCCGGCGGATCTGGCCCACTGCGCCAAGCTG
GCCCGGGAGCGCGGCTATGACGAGATCAACCTCAACGTCGGCTGCCCGTCCGATCGGGTTCAAAATGGCCGCTTCGGTGC
CTGCCTGATGGGTGAACCGGCGCTGGTGGCCGACTGCGTCAAGGCGATGCGCGATGTGGTCGATATCCCGGTGACGGTCA
AGACCCGGATCGGTATCGACGATCAGGACTCCTACGAATTTTTGCAGACCTTTATCGAGCAGGTGCGCGATGCCGGTTGC
GATACCTTCATCGTCCATGCCCGCAAGGCATGGCTCAGTGGTCTGAGCCCACGTGAAAACCGCGAAATCCCGCCACTCGA
CTATCCCCGCGTCTATCGGGTCAAACAGGATTATCCCGAGCTGACCATAGCCCTCAACGGCGGTGTCACCAGCCTCGAGC
AAACGCTGGAACATCTGCAGCAGGTGGATGGGGTCATGATGGGGCGTGAGGCCTACCAGAACCCCTACATACTGGCGCAG
GTAGACAGCCAGCTGTTCGGTCTGGCGAGCGAGATCCCGAGCCGCCATGAGGTGGTGCGAATGATGCTCCCCTACATGGA
GCAGGAGCTGGCCAAAGGCAACTACCTCTCCCACATGACCCGCCACATGCTGGGCCTGTTCCAGAACATGCAGGGAGCCC
GTGCCTGGCGTCGTCACCTTAGCGAGAACGCCTGCAAGCAGGGCGCCGGCATTCAAGTCGTGCTGGATGCCATGGCCAAG
GTGCCGGAATTCGCCAGCGCCGAATCGGTAGAGTAA

Upstream 100 bases:

>100_bases
GATACTACCACAGGGCCTCGCATGGCGCGGCGACAAAATTCATTTTCTGGGGCTGCCTCACGTTGTGATAAAATCCTGCC
CCTTTGTTTAGCCAACCCGG

Downstream 100 bases:

>100_bases
TCATGTACCGCTTCACGGGTTCATGGCTGCACAAGGCAGTCGTCCGGCTCTGCGCCGGACTGTTGGCCATCCTCGGCATC
AAGCTCAACGCCTTCTACTT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIFMQAQRFSIAPMLDWTDRHCRYFHRLMTRQTLLYTEMVTTGAIIHGKGDYLGYSEQERPVSLQLGGSNPADLAHCAKL
ARERGYDEINLNVGCPSDRVQNGRFGACLMGEPALVADCVKAMRDVVDIPVTVKTRIGIDDQDSYEFLQTFIEQVRDAGC
DTFIVHARKAWLSGLSPRENREIPPLDYPRVYRVKQDYPELTIALNGGVTSLEQTLEHLQQVDGVMMGREAYQNPYILAQ
VDSQLFGLASEIPSRHEVVRMMLPYMEQELAKGNYLSHMTRHMLGLFQNMQGARAWRRHLSENACKQGAGIQVVLDAMAK
VPEFASAESVE

Sequences:

>Translated_331_residues
MIFMQAQRFSIAPMLDWTDRHCRYFHRLMTRQTLLYTEMVTTGAIIHGKGDYLGYSEQERPVSLQLGGSNPADLAHCAKL
ARERGYDEINLNVGCPSDRVQNGRFGACLMGEPALVADCVKAMRDVVDIPVTVKTRIGIDDQDSYEFLQTFIEQVRDAGC
DTFIVHARKAWLSGLSPRENREIPPLDYPRVYRVKQDYPELTIALNGGVTSLEQTLEHLQQVDGVMMGREAYQNPYILAQ
VDSQLFGLASEIPSRHEVVRMMLPYMEQELAKGNYLSHMTRHMLGLFQNMQGARAWRRHLSENACKQGAGIQVVLDAMAK
VPEFASAESVE
>Mature_331_residues
MIFMQAQRFSIAPMLDWTDRHCRYFHRLMTRQTLLYTEMVTTGAIIHGKGDYLGYSEQERPVSLQLGGSNPADLAHCAKL
ARERGYDEINLNVGCPSDRVQNGRFGACLMGEPALVADCVKAMRDVVDIPVTVKTRIGIDDQDSYEFLQTFIEQVRDAGC
DTFIVHARKAWLSGLSPRENREIPPLDYPRVYRVKQDYPELTIALNGGVTSLEQTLEHLQQVDGVMMGREAYQNPYILAQ
VDSQLFGLASEIPSRHEVVRMMLPYMEQELAKGNYLSHMTRHMLGLFQNMQGARAWRRHLSENACKQGAGIQVVLDAMAK
VPEFASAESVE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=183, Percent_Identity=28.4153005464481, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239788462, Length=183, Percent_Identity=28.4153005464481, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI145693211, Length=317, Percent_Identity=71.9242902208202, Blast_Score=479, Evalue=1e-137,
Organism=Escherichia coli, GI1789660, Length=300, Percent_Identity=24.3333333333333, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788462, Length=224, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=26.8867924528302, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=250, Percent_Identity=24.8, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24580595, Length=254, Percent_Identity=24.0157480314961, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19920448, Length=254, Percent_Identity=24.0157480314961, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37418; Mature: 37418

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFMQAQRFSIAPMLDWTDRHCRYFHRLMTRQTLLYTEMVTTGAIIHGKGDYLGYSEQER
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCC
PVSLQLGGSNPADLAHCAKLARERGYDEINLNVGCPSDRVQNGRFGACLMGEPALVADCV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEECCCCHHHHHHH
KAMRDVVDIPVTVKTRIGIDDQDSYEFLQTFIEQVRDAGCDTFIVHARKAWLSGLSPREN
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
REIPPLDYPRVYRVKQDYPELTIALNGGVTSLEQTLEHLQQVDGVMMGREAYQNPYILAQ
CCCCCCCCCHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VDSQLFGLASEIPSRHEVVRMMLPYMEQELAKGNYLSHMTRHMLGLFQNMQGARAWRRHL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
SENACKQGAGIQVVLDAMAKVPEFASAESVE
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIFMQAQRFSIAPMLDWTDRHCRYFHRLMTRQTLLYTEMVTTGAIIHGKGDYLGYSEQER
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCC
PVSLQLGGSNPADLAHCAKLARERGYDEINLNVGCPSDRVQNGRFGACLMGEPALVADCV
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEECCCCHHHHHHH
KAMRDVVDIPVTVKTRIGIDDQDSYEFLQTFIEQVRDAGCDTFIVHARKAWLSGLSPREN
HHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
REIPPLDYPRVYRVKQDYPELTIALNGGVTSLEQTLEHLQQVDGVMMGREAYQNPYILAQ
CCCCCCCCCHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
VDSQLFGLASEIPSRHEVVRMMLPYMEQELAKGNYLSHMTRHMLGLFQNMQGARAWRRHL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
SENACKQGAGIQVVLDAMAKVPEFASAESVE
HHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]