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The map label for this gene is leuB [H]

Identifier: 145300292

GI number: 145300292

Start: 3691128

End: 3692213

Strand: Direct

Name: leuB [H]

Synonym: ASA_3410

Alternate gene names: 145300292

Gene position: 3691128-3692213 (Clockwise)

Preceding gene: 145300291

Following gene: 145300293

Centisome position: 78.49

GC content: 62.15

Gene sequence:

>1086_bases
ATGAGCAGTTATCGGATCGCAGTGTTGCCGGGTGACGGCATTGGCCCGGAAGTGATGGCCGAGGCCGTCAAGGTGCTGGA
AAAAGTGCAGGCCAAGTTTGGCTTCTCCCTCGAGTATGATCGCCATGACGTGGGTGGCATCGCCATCGACAACCATGGCA
CCCCGCTGCCACAAAGCACCATCAAGGGCTGTGAGGCGGCCGATGCCGTGCTGTTTGGCTCCGTAGGTGGCCCCAAATGG
GAGCACCTGGCCCCCAACGATCAACCCGAGCGCGGCGCCCTGCTGCCCCTGCGTGCCCACTTCAAGCTGTTCTGCAACCT
GCGTCCGGCCCGTATCTACACAGGTCTTGAGCAGTTCTCCCCCCTGCGCGCCGACATCTCGGATCGCGGTTTTGACATCG
CCTGCGTGCGGGAGCTGACCGGCGGCATCTACTTCGGCCAGCCCAAGGGGCGCGAGGGAGAAGGGGCGAACGAGAAAGCA
TTCGACACCGAGGTGTACCATCGCTTCGAAATTGAACGCATCGCCAGGATAGCGTTTGAATCGGCCCGTGTTCGCAAGGC
CAAGGTCACCTCCATCGACAAGGCCAACGTGCTCGCCTCCTCCATTCTGTGGCGCGAGGTGGTGACGCAAGTCGCCAAAT
CCTACCCGGACGTGGCGCTGAACCACATGTACATCGACAACGCCACCATGCAGCTCATCAAGGATCCGTCCCAGTTCGAC
GTGCTGCTCTGCTCCAACCTGTTTGGCGACATCCTCTCTGACGAGTGCGCCATGATCACCGGCTCCATGGGGTTGCTCCC
TTCAGCCAGCCTGAACGAATCCGGTTTTGGCCTGTTCGAACCGGCCGGTGGCTCGGCCCCCGATATTGCCGGCAAGGGGA
TCGCCAATCCCATCGCCCAGATCCTCTCCGCCGCCCTGATGCTGCGCTACAGCCTGGGACAGGAAGCCGCCGCTCAAGCC
ATCGAGCAAGCCGTAGCCCAGACATTGGCAGAGGGATACTTCACCGCCGACCTGCATCAGGCAGATGCTCGCCATCCGGT
GCAGAGTACCGCCGACATGGGCACCCAGATCGCCGCACGGATCTGA

Upstream 100 bases:

>100_bases
TCCACGTAATCAACAGCATCTGGCGCGCCGATCAGGTGGCCGAACAGATGGAACGCAACGTCACAAAAACAGACAAGATC
AATACGGAGAGTGTGTGAGT

Downstream 100 bases:

>100_bases
GCCACCCAGCACATAACAAGGAATACAGAGCAATGGCCAAAACCCTTTATCAGAAAGTATTCGACGCCCACGTGGTACGC
GAAGTGGACGGCGAAACGCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSSYRIAVLPGDGIGPEVMAEAVKVLEKVQAKFGFSLEYDRHDVGGIAIDNHGTPLPQSTIKGCEAADAVLFGSVGGPKW
EHLAPNDQPERGALLPLRAHFKLFCNLRPARIYTGLEQFSPLRADISDRGFDIACVRELTGGIYFGQPKGREGEGANEKA
FDTEVYHRFEIERIARIAFESARVRKAKVTSIDKANVLASSILWREVVTQVAKSYPDVALNHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGLLPSASLNESGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRYSLGQEAAAQA
IEQAVAQTLAEGYFTADLHQADARHPVQSTADMGTQIAARI

Sequences:

>Translated_361_residues
MSSYRIAVLPGDGIGPEVMAEAVKVLEKVQAKFGFSLEYDRHDVGGIAIDNHGTPLPQSTIKGCEAADAVLFGSVGGPKW
EHLAPNDQPERGALLPLRAHFKLFCNLRPARIYTGLEQFSPLRADISDRGFDIACVRELTGGIYFGQPKGREGEGANEKA
FDTEVYHRFEIERIARIAFESARVRKAKVTSIDKANVLASSILWREVVTQVAKSYPDVALNHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGLLPSASLNESGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRYSLGQEAAAQA
IEQAVAQTLAEGYFTADLHQADARHPVQSTADMGTQIAARI
>Mature_360_residues
SSYRIAVLPGDGIGPEVMAEAVKVLEKVQAKFGFSLEYDRHDVGGIAIDNHGTPLPQSTIKGCEAADAVLFGSVGGPKWE
HLAPNDQPERGALLPLRAHFKLFCNLRPARIYTGLEQFSPLRADISDRGFDIACVRELTGGIYFGQPKGREGEGANEKAF
DTEVYHRFEIERIARIAFESARVRKAKVTSIDKANVLASSILWREVVTQVAKSYPDVALNHMYIDNATMQLIKDPSQFDV
LLCSNLFGDILSDECAMITGSMGLLPSASLNESGFGLFEPAGGSAPDIAGKGIANPIAQILSAALMLRYSLGQEAAAQAI
EQAVAQTLAEGYFTADLHQADARHPVQSTADMGTQIAARI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=34.2261904761905, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=30.7936507936508, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178816, Length=315, Percent_Identity=30.7936507936508, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=27.906976744186, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178838, Length=334, Percent_Identity=27.8443113772455, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178819, Length=151, Percent_Identity=37.0860927152318, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=75.2808988764045, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1788101, Length=349, Percent_Identity=38.9684813753582, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787381, Length=124, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71986051, Length=331, Percent_Identity=35.0453172205438, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=345, Percent_Identity=31.5942028985507, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=30.5475504322767, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=347, Percent_Identity=29.3948126801153, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=368, Percent_Identity=42.3913043478261, Blast_Score=266, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=36.3636363636364, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6324709, Length=335, Percent_Identity=32.5373134328358, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=340, Percent_Identity=31.1764705882353, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24643268, Length=337, Percent_Identity=33.8278931750742, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24643270, Length=339, Percent_Identity=33.6283185840708, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=30.9941520467836, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI161078635, Length=331, Percent_Identity=30.8157099697885, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI161078633, Length=331, Percent_Identity=30.8157099697885, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24650122, Length=331, Percent_Identity=30.8157099697885, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=30.6306306306306, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078639, Length=329, Percent_Identity=31.0030395136778, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=336, Percent_Identity=24.1071428571429, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648872, Length=336, Percent_Identity=24.1071428571429, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20130355, Length=326, Percent_Identity=23.3128834355828, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38881; Mature: 38749

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYRIAVLPGDGIGPEVMAEAVKVLEKVQAKFGFSLEYDRHDVGGIAIDNHGTPLPQST
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEECCCCCCCCHHH
IKGCEAADAVLFGSVGGPKWEHLAPNDQPERGALLPLRAHFKLFCNLRPARIYTGLEQFS
HCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHC
PLRADISDRGFDIACVRELTGGIYFGQPKGREGEGANEKAFDTEVYHRFEIERIARIAFE
CHHCCCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SARVRKAKVTSIDKANVLASSILWREVVTQVAKSYPDVALNHMYIDNATMQLIKDPSQFD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCHHHHHHCCCCHHH
VLLCSNLFGDILSDECAMITGSMGLLPSASLNESGFGLFEPAGGSAPDIAGKGIANPIAQ
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
ILSAALMLRYSLGQEAAAQAIEQAVAQTLAEGYFTADLHQADARHPVQSTADMGTQIAAR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEHHHHHCCCCCCCHHHHHHHHHHHHC
I
C
>Mature Secondary Structure 
SSYRIAVLPGDGIGPEVMAEAVKVLEKVQAKFGFSLEYDRHDVGGIAIDNHGTPLPQST
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEECCCCCCCCHHH
IKGCEAADAVLFGSVGGPKWEHLAPNDQPERGALLPLRAHFKLFCNLRPARIYTGLEQFS
HCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHC
PLRADISDRGFDIACVRELTGGIYFGQPKGREGEGANEKAFDTEVYHRFEIERIARIAFE
CHHCCCCCCCCCHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SARVRKAKVTSIDKANVLASSILWREVVTQVAKSYPDVALNHMYIDNATMQLIKDPSQFD
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCHHHHHHCCCCHHH
VLLCSNLFGDILSDECAMITGSMGLLPSASLNESGFGLFEPAGGSAPDIAGKGIANPIAQ
HHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHH
ILSAALMLRYSLGQEAAAQAIEQAVAQTLAEGYFTADLHQADARHPVQSTADMGTQIAAR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEHHHHHCCCCCCCHHHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA