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The map label for this gene is leuA [H]

Identifier: 145300291

GI number: 145300291

Start: 3689551

End: 3691125

Strand: Direct

Name: leuA [H]

Synonym: ASA_3409

Alternate gene names: 145300291

Gene position: 3689551-3691125 (Clockwise)

Preceding gene: 145300284

Following gene: 145300292

Centisome position: 78.46

GC content: 59.56

Gene sequence:

>1575_bases
ATGTCAGATCGGGTCATCATTTTCGACACCACCTTGCGCGATGGTGAGCAGGCCCTGGCGGCCAGTTTGACCGTCAAGGA
GAAGCTGCAGATCGCCCAGGCGCTGGAGCGGCTCGGCGTCGACATCATGGAAGTGGGATTCCCTGTCTCCTCCCCCGGCG
ATTTTCAATCGGTGCAGACCATAGCCCGCCACATCAAGAACAGCCGGGTCTGCGCCCTCGCCCGCGCCCTGCCCCAGGAT
ATCGACGCCGCCGGTGAAGCCTTGCGGGTCGCAGAAGCTTTTCGCATCCATACCTTTATCTCCACCTCCTCCATTCACGT
CGAGAGCAAGCTGAAAAAGAGCTTCGAGGATGTGCTGGAGATGGGGGTCAGCGCCATCAAGCACGCCTTGCGCTACACCG
ACGATGTAGAGTTCTCCTGCGAGGATGCGGGTCGCACCCCGATTGATAATCTCTGTCGCATGGTGGAAGCGGCCATCAAG
GCCGGCGCCCGCACCATCAATATCCCGGATACCGTCGGCTACACGGTACCGACCGAGTTCTCCGGTATCATCCAGACCCT
GTTCAACCGGGTGCCCAACATCGACAAGGCCATCATCTCTGTGCACTGCCACGATGACCTGGGACTGTCGGTGGCCAACT
CCATCGGCGCCGTGCAGATGGGAGCCCGCCAGATCGAGTGCACCATCAACGGCATCGGCGAGCGGGCCGGCAACTGCTCC
CTGGAAGAGGTCGCGATGATCCTGAAAACCCGCGCCGACCTGCTTGGGGTCCATACCAACATCCGCCACAGCGAAATTCA
TCGCACCAGCGCCCTGGTCAGCCAGCTGTGCAACATGCCGGTGCAGCCGAACAAGGCCATCGTCGGGGCCAACGCCTTCT
CCCACAGCTCAGGCATCCATCAGGACGGCGTGCTCAAGGCGAAGAACACCTATGAAATCATCACCCCCGAGAGCATTGGC
CTGCCCCAGAACAACCTCAACATGACCTCCCGCTCCGGCCGTCACGTCATCAAGCACAGAATGGAGTCCATGGGTTATGC
CGAGAGCAGCTACGATCTCGACCACCTCTACGGCAAGTTCCTGACCCTGGCCGACAAGAAGGGGCAAGTATTCGACTACG
ACCTGGAGGCGCTGGCCTTCTTCAGCCAAATCCACGAAGAGCCGGAGCACTTCAAGCTGGAGTACCTGGGCGTACAAAGC
GGCAGCTCTGTGCTGGCCACCGCCTCGGTCAAATTGAAGGTCGGGCAGGATCTGGTCTGCGAAGCGGCTACCGGCAATGG
TCCTGTGGATGCGGTCTACCAGTGCATCAACCGCATCACCGGCTACGAGATCCGCATCGACAAATACGCCCTCAAAGCCA
AGGGTGAGGGCAAGAATGCCCTCGGCCAGGTCGATATCGTCGCCGAATACAAGGGACGAAAATTCCACGGCATGGGCCTG
GCTACCGATATCATAGAGTCCTCTGCCCAGGCGTTGATCCACGTAATCAACAGCATCTGGCGCGCCGATCAGGTGGCCGA
ACAGATGGAACGCAACGTCACAAAAACAGACAAGATCAATACGGAGAGTGTGTGA

Upstream 100 bases:

>100_bases
TCAGGCATAGCCGGAATTTACAAAAACCCGTGCACCCTGCACGGGTTTTTTTATGCCGCCGCAGGATGCACGTTACCGAA
CTTAGGAAGAGGGAAGTCAC

Downstream 100 bases:

>100_bases
GTATGAGCAGTTATCGGATCGCAGTGTTGCCGGGTGACGGCATTGGCCCGGAAGTGATGGCCGAGGCCGTCAAGGTGCTG
GAAAAAGTGCAGGCCAAGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQTIARHIKNSRVCALARALPQD
IDAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLEMGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIK
AGARTINIPDTVGYTVPTEFSGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCS
LEEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIHQDGVLKAKNTYEIITPESIG
LPQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKFLTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQS
GSSVLATASVKLKVGQDLVCEAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGL
ATDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV

Sequences:

>Translated_524_residues
MSDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQTIARHIKNSRVCALARALPQD
IDAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLEMGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIK
AGARTINIPDTVGYTVPTEFSGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCS
LEEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIHQDGVLKAKNTYEIITPESIG
LPQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKFLTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQS
GSSVLATASVKLKVGQDLVCEAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGL
ATDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV
>Mature_523_residues
SDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQTIARHIKNSRVCALARALPQDI
DAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLEMGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIKA
GARTINIPDTVGYTVPTEFSGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCSL
EEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIHQDGVLKAKNTYEIITPESIGL
PQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKFLTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQSG
SSVLATASVKLKVGQDLVCEAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGLA
TDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=524, Percent_Identity=68.8931297709924, Blast_Score=763, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=543, Percent_Identity=25.9668508287293, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320071, Length=360, Percent_Identity=26.9444444444444, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=27.1468144044321, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324682, Length=540, Percent_Identity=24.2592592592593, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57249; Mature: 57118

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHCCCCCCCCCHHHHHH
IARHIKNSRVCALARALPQDIDAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLE
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEHHHHHHHHHHHHHH
MGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIKAGARTINIPDTVGYTVPTEF
HHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
SGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHCCCHHHHCCEEEEEEEECCCCCCCCCC
LEEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKAKNTYEIITPESIGLPQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKF
CCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQSGSSVLATASVKLKVGQDLVC
HHEECCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCEEE
EAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGL
EECCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCEEECCCH
ATDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SDRVIIFDTTLRDGEQALAASLTVKEKLQIAQALERLGVDIMEVGFPVSSPGDFQSVQT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHCCCCCCCCCHHHHHH
IARHIKNSRVCALARALPQDIDAAGEALRVAEAFRIHTFISTSSIHVESKLKKSFEDVLE
HHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEHHHHHHHHHHHHHH
MGVSAIKHALRYTDDVEFSCEDAGRTPIDNLCRMVEAAIKAGARTINIPDTVGYTVPTEF
HHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHH
SGIIQTLFNRVPNIDKAIISVHCHDDLGLSVANSIGAVQMGARQIECTINGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHCCCHHHHCCEEEEEEEECCCCCCCCCC
LEEVAMILKTRADLLGVHTNIRHSEIHRTSALVSQLCNMPVQPNKAIVGANAFSHSSGIH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKAKNTYEIITPESIGLPQNNLNMTSRSGRHVIKHRMESMGYAESSYDLDHLYGKF
CCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LTLADKKGQVFDYDLEALAFFSQIHEEPEHFKLEYLGVQSGSSVLATASVKLKVGQDLVC
HHEECCCCCEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCEEE
EAATGNGPVDAVYQCINRITGYEIRIDKYALKAKGEGKNALGQVDIVAEYKGRKFHGMGL
EECCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCEEEEEECCCCEEECCCH
ATDIIESSAQALIHVINSIWRADQVAEQMERNVTKTDKINTESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA