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The map label for this gene is rnhA [H]

Identifier: 145299713

GI number: 145299713

Start: 2987487

End: 2987951

Strand: Direct

Name: rnhA [H]

Synonym: ASA_2788

Alternate gene names: 145299713

Gene position: 2987487-2987951 (Clockwise)

Preceding gene: 145299710

Following gene: 145299715

Centisome position: 63.53

GC content: 58.92

Gene sequence:

>465_bases
ATGTTAAAACACATTGATTTATACACGGATGGTTCCTGCCTCGGTAATCCCGGCCCGGGTGGCTATGGCGCCGTCCTGGT
CTATGGCGATCACCGCAAGGAGATTTCCGGCGGTTTTCGGTTGACCACCAATAACCGGATGGAGCTGATGGCCGCCATCA
TGGGGCTGCGTACCCTCAATGCCGCCTGCCAGGTACGGCTTACCACCGACAGCCAGTATGTGCGCCAGGGGATCACCCAA
TGGATCATCGGCTGGAAGAAGAAGGGGTGGATGACCTCAAATCGTCAGCCGGTCAAGAACGTCGATCTCTGGAAAGAGCT
GGATGCCGAAGTGGCCCGCCATCAGATCGAGTGGCTCTGGGTAAAGGGGCACTCGGGTCACCCCGAGAACGAACGCTGCG
ATGAACTGGCGCGCGATGCCGCCAGCGGCAAAGAACTCGCCGAAGATACCGGCTACCAGCCCTGA

Upstream 100 bases:

>100_bases
GGCGATTCAGTTGGGGTGTGTTCATGATCCAATCTATGTCAAACTTCGGCCTTACCCGCTAGTTTACCCCAAGACTCACG
CTAAAAAAGATCGATTGCTC

Downstream 100 bases:

>100_bases
CGTGCCATGCCCGGTGCCGCTATCGACTCAGGCAAACGCCAGCGACGACGCACCGGGATGAGCGGAAAACGCCGTTTGCG
CGCCGCTATTACATAGACGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLKHIDLYTDGSCLGNPGPGGYGAVLVYGDHRKEISGGFRLTTNNRMELMAAIMGLRTLNAACQVRLTTDSQYVRQGITQ
WIIGWKKKGWMTSNRQPVKNVDLWKELDAEVARHQIEWLWVKGHSGHPENERCDELARDAASGKELAEDTGYQP

Sequences:

>Translated_154_residues
MLKHIDLYTDGSCLGNPGPGGYGAVLVYGDHRKEISGGFRLTTNNRMELMAAIMGLRTLNAACQVRLTTDSQYVRQGITQ
WIIGWKKKGWMTSNRQPVKNVDLWKELDAEVARHQIEWLWVKGHSGHPENERCDELARDAASGKELAEDTGYQP
>Mature_154_residues
MLKHIDLYTDGSCLGNPGPGGYGAVLVYGDHRKEISGGFRLTTNNRMELMAAIMGLRTLNAACQVRLTTDSQYVRQGITQ
WIIGWKKKGWMTSNRQPVKNVDLWKELDAEVARHQIEWLWVKGHSGHPENERCDELARDAASGKELAEDTGYQP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=36.4238410596026, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1786408, Length=153, Percent_Identity=67.3202614379085, Blast_Score=220, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=36.3636363636364, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17505482, Length=133, Percent_Identity=40.6015037593985, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=37.6712328767123, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=37.6712328767123, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17327; Mature: 17327

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHIDLYTDGSCLGNPGPGGYGAVLVYGDHRKEISGGFRLTTNNRMELMAAIMGLRTLN
CCCEEEEEECCCCCCCCCCCCEEEEEEECCCCHHCCCCEEEECCCHHHHHHHHHHHHHCC
AACQVRLTTDSQYVRQGITQWIIGWKKKGWMTSNRQPVKNVDLWKELDAEVARHQIEWLW
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
VKGHSGHPENERCDELARDAASGKELAEDTGYQP
EECCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MLKHIDLYTDGSCLGNPGPGGYGAVLVYGDHRKEISGGFRLTTNNRMELMAAIMGLRTLN
CCCEEEEEECCCCCCCCCCCCEEEEEEECCCCHHCCCCEEEECCCHHHHHHHHHHHHHCC
AACQVRLTTDSQYVRQGITQWIIGWKKKGWMTSNRQPVKNVDLWKELDAEVARHQIEWLW
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEE
VKGHSGHPENERCDELARDAASGKELAEDTGYQP
EECCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA