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The map label for this gene is gloB [H]

Identifier: 145299715

GI number: 145299715

Start: 2988733

End: 2989497

Strand: Direct

Name: gloB [H]

Synonym: ASA_2790

Alternate gene names: 145299715

Gene position: 2988733-2989497 (Clockwise)

Preceding gene: 145299713

Following gene: 145299716

Centisome position: 63.56

GC content: 56.6

Gene sequence:

>765_bases
ATGTATACCGTTATCACAGTTCCTGCCTTTACCGACAATTACATCTGGTTGATCAGACACGAAAATCACTGCTTGGTGGT
CGACCCGGGCGATGCAGGCCCTGTGCTGGATCGGCTGGCTGCACTGGACTTGCAGCTCGATGCCATCTTGCTGACTCACC
ACCACCAGGATCATGTGGGCGGCGTCACCGCCCTGCTCAAGCATTTCCCCCATGCCAGATTGTATGGTCCCAAACATGAC
CCCATGCCTGATCACCATGGCCAGTGGCTCGATGACGGGGATCAGATCAACTGGCATGGTTTGAGCCTGGATGTCATTCA
TGTGCCCGGTCATACCCATGGCCATATTGCCTATCACGGCCATGGCATGCTGTTTTGCGGTGATACGCTCTTCTCCGGCG
GTTGCGGTCGCCTGTTCGAAGGCACCCCGGCCCAGATGCATGACTCGCTGCAGCGGCTCGCGGCCCTGCCCGACGACACC
CTCATCTATTGTGCCCACGAATACACTCTCTCCAACCTGCGCTTCGCCTACGCAGTCGAGCCCGACAACAAGGCGATCCA
GCAGCGGATCGGCATGATCAGCAAACTGCGCCAGCAGGGCCTGCCGAGTCTCCCTTCTCGCCTGGGCGATGAGCGCGATT
TCAACGTTTTTCTGCGTTGCGAGCAGGATTCCGTGAAATTTTCTGCTGAAAAATACGCCTTAAAATGCTTGGAAAATCCA
GAGGATACCTTCGCCGTCCTGCGCAGCTGGAAGGACGTTTTTTGA

Upstream 100 bases:

>100_bases
ATTTGTTGTTCGGTACGTGCCAACTGCATAATGGGGCTCAACTTGGGGTGCGAACGCCATCCAGTTATATAAGATACCCT
CCCTAAAGAGAAGCTAACTC

Downstream 100 bases:

>100_bases
ACGAACCTTGATCAGGGGGCCATTGCCCCTTATGATGCCAGCTGTTTTTCAAGAGATGACCTGAGCGAGATGAAGGTACG
AACGGCCTTATTGGCGGCGC

Product: hydroxyacylglutathione hydrolase GloB

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MYTVITVPAFTDNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVGGVTALLKHFPHARLYGPKHD
PMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHGHGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDT
LIYCAHEYTLSNLRFAYAVEPDNKAIQQRIGMISKLRQQGLPSLPSRLGDERDFNVFLRCEQDSVKFSAEKYALKCLENP
EDTFAVLRSWKDVF

Sequences:

>Translated_254_residues
MYTVITVPAFTDNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVGGVTALLKHFPHARLYGPKHD
PMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHGHGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDT
LIYCAHEYTLSNLRFAYAVEPDNKAIQQRIGMISKLRQQGLPSLPSRLGDERDFNVFLRCEQDSVKFSAEKYALKCLENP
EDTFAVLRSWKDVF
>Mature_254_residues
MYTVITVPAFTDNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVGGVTALLKHFPHARLYGPKHD
PMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHGHGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDT
LIYCAHEYTLSNLRFAYAVEPDNKAIQQRIGMISKLRQQGLPSLPSRLGDERDFNVFLRCEQDSVKFSAEKYALKCLENP
EDTFAVLRSWKDVF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=261, Percent_Identity=33.7164750957854, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI94538322, Length=261, Percent_Identity=33.7164750957854, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI116642887, Length=225, Percent_Identity=37.3333333333333, Blast_Score=137, Evalue=7e-33,
Organism=Homo sapiens, GI21703352, Length=225, Percent_Identity=37.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI14150041, Length=245, Percent_Identity=33.469387755102, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI46361987, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI41327741, Length=186, Percent_Identity=30.1075268817204, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=50.199203187251, Blast_Score=226, Evalue=8e-61,
Organism=Escherichia coli, GI1787158, Length=191, Percent_Identity=31.413612565445, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=238, Percent_Identity=33.6134453781513, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538952, Length=173, Percent_Identity=32.3699421965318, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320478, Length=246, Percent_Identity=29.6747967479675, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324614, Length=244, Percent_Identity=28.2786885245902, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356335, Length=259, Percent_Identity=39.7683397683398, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24667703, Length=259, Percent_Identity=39.7683397683398, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24667711, Length=259, Percent_Identity=39.7683397683398, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI221330176, Length=169, Percent_Identity=30.1775147928994, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28639; Mature: 28639

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTVITVPAFTDNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVG
CEEEEEECCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHH
GVTALLKHFPHARLYGPKHDPMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHG
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEECC
HGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDTLIYCAHEYTLSNLRFAYAVE
CEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEEC
PDNKAIQQRIGMISKLRQQGLPSLPSRLGDERDFNVFLRCEQDSVKFSAEKYALKCLENP
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCC
EDTFAVLRSWKDVF
HHHHHHHHHHHHCC
>Mature Secondary Structure
MYTVITVPAFTDNYIWLIRHENHCLVVDPGDAGPVLDRLAALDLQLDAILLTHHHQDHVG
CEEEEEECCCCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHH
GVTALLKHFPHARLYGPKHDPMPDHHGQWLDDGDQINWHGLSLDVIHVPGHTHGHIAYHG
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCEEEECC
HGMLFCGDTLFSGGCGRLFEGTPAQMHDSLQRLAALPDDTLIYCAHEYTLSNLRFAYAVE
CEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEEEEEC
PDNKAIQQRIGMISKLRQQGLPSLPSRLGDERDFNVFLRCEQDSVKFSAEKYALKCLENP
CCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEECCCCCEEHHHHHHHHHHCCC
EDTFAVLRSWKDVF
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA