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The map label for this gene is glpF [H]

Identifier: 145299635

GI number: 145299635

Start: 2907950

End: 2908798

Strand: Direct

Name: glpF [H]

Synonym: ASA_2709

Alternate gene names: 145299635

Gene position: 2907950-2908798 (Clockwise)

Preceding gene: 145299628

Following gene: 145299636

Centisome position: 61.84

GC content: 61.72

Gene sequence:

>849_bases
ATGAGCATACAAAGACCCAATACCCTGCTTGGCGAGTGCATCGCCGAGTTTATCGGAACCGGCCTGCTGATATTTTTCGG
CGTCGGTTGTGTGGCCGCCCTGGTGCTGGCCGGTGCCAACTTCGGACAATGGGAAATCTCCATTACCTGGGGTCTGGGTG
TCGCCATCGCCATCTACGTGACCGGCGGTATTTCTGGTGCCCACCTGAACCCGGCCGTGACCCTGGCGCTGATGACCTTC
TGCGGGTTCGACAAGCGCAAAGTGGTGCCCTACATCATCGCCCAGGTCGCTGGCGCCTTCTGCTTCGCCGCCCTTGTCTA
CTTCCTCTACGGCGGCCTGTTCACCCAGTGGGAAGTGACCCACAACGTGGTACGTGGCAGCGTCGAGAGCCTCGGCTCCG
CCGGCATCTTCTCCACCTACCCGAACGCCCTGCTCTCCAACATGCAAGCCTTCGCGGTCGAGCTGGTGATCACTGCCGTC
CTGATGCTGGGCATCATGGCGCTGGGTGACAACAACAACGGCGCCCCCAAAGGCTTCGCCGCCGCCCTGCTGATCGGTAT
CCTGATCGCCGTGATCGGTGCCTCCCTCGGCCCGTTAACCGGCTTTGCCATGAACCCTGCCCGTGATTTCGGCCCCAAAC
TGTTTGCCTTCCTGGCAGGCTGGGGTGACGTGGCCATGACTGGTGGTCGTGACAACCCCTACTTCTGGGTGCCCATCCTT
GGCCCCATCGTCGGTGCCCAGCTTGGTACCGCCCTGTATGTGAAAGTGTTGGCTCCCTGTGTGCCTGGCAACCGTGTTGC
CGCCACCGAGCAAGCTGCCCAAACCGCCAAAGAAGAGGCCGCAGCATAA

Upstream 100 bases:

>100_bases
CAAAAAACAAGAAAAAACGTTTTCGGATGCAGTAATGGGACTGAAAAAACAACAAAACGGAACATCAAACCAACTCTGGG
TGGATATAAGGAATAAAAGT

Downstream 100 bases:

>100_bases
GAGCGGCCCTCCCCAACGTGTAACAACGTGGAAAATAAAAACGTGGAGAACATCATGTCTGCTGAAAAAAAATATGTCGT
CGCTCTGGACCAGGGTACTA

Product: glycerol uptake facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSIQRPNTLLGECIAEFIGTGLLIFFGVGCVAALVLAGANFGQWEISITWGLGVAIAIYVTGGISGAHLNPAVTLALMTF
CGFDKRKVVPYIIAQVAGAFCFAALVYFLYGGLFTQWEVTHNVVRGSVESLGSAGIFSTYPNALLSNMQAFAVELVITAV
LMLGIMALGDNNNGAPKGFAAALLIGILIAVIGASLGPLTGFAMNPARDFGPKLFAFLAGWGDVAMTGGRDNPYFWVPIL
GPIVGAQLGTALYVKVLAPCVPGNRVAATEQAAQTAKEEAAA

Sequences:

>Translated_282_residues
MSIQRPNTLLGECIAEFIGTGLLIFFGVGCVAALVLAGANFGQWEISITWGLGVAIAIYVTGGISGAHLNPAVTLALMTF
CGFDKRKVVPYIIAQVAGAFCFAALVYFLYGGLFTQWEVTHNVVRGSVESLGSAGIFSTYPNALLSNMQAFAVELVITAV
LMLGIMALGDNNNGAPKGFAAALLIGILIAVIGASLGPLTGFAMNPARDFGPKLFAFLAGWGDVAMTGGRDNPYFWVPIL
GPIVGAQLGTALYVKVLAPCVPGNRVAATEQAAQTAKEEAAA
>Mature_281_residues
SIQRPNTLLGECIAEFIGTGLLIFFGVGCVAALVLAGANFGQWEISITWGLGVAIAIYVTGGISGAHLNPAVTLALMTFC
GFDKRKVVPYIIAQVAGAFCFAALVYFLYGGLFTQWEVTHNVVRGSVESLGSAGIFSTYPNALLSNMQAFAVELVITAVL
MLGIMALGDNNNGAPKGFAAALLIGILIAVIGASLGPLTGFAMNPARDFGPKLFAFLAGWGDVAMTGGRDNPYFWVPILG
PIVGAQLGTALYVKVLAPCVPGNRVAATEQAAQTAKEEAAA

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=278, Percent_Identity=40.2877697841727, Blast_Score=167, Evalue=7e-42,
Organism=Homo sapiens, GI4826645, Length=278, Percent_Identity=37.7697841726619, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI22538420, Length=279, Percent_Identity=37.2759856630824, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4502187, Length=271, Percent_Identity=34.3173431734317, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI4502179, Length=249, Percent_Identity=36.144578313253, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI310133356, Length=178, Percent_Identity=33.7078651685393, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310114181, Length=178, Percent_Identity=33.7078651685393, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI86792455, Length=250, Percent_Identity=38, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4502183, Length=266, Percent_Identity=31.5789473684211, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI6912506, Length=256, Percent_Identity=31.640625, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4755125, Length=234, Percent_Identity=32.9059829059829, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4502181, Length=234, Percent_Identity=32.9059829059829, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI37694062, Length=252, Percent_Identity=28.968253968254, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1790362, Length=280, Percent_Identity=65.3571428571429, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787101, Length=210, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=280, Percent_Identity=32.8571428571429, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=37.5, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531429, Length=292, Percent_Identity=33.9041095890411, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=33.8645418326693, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531431, Length=287, Percent_Identity=33.7979094076655, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17533613, Length=252, Percent_Identity=34.5238095238095, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564052, Length=252, Percent_Identity=34.5238095238095, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=31.4741035856574, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558372, Length=259, Percent_Identity=25.4826254826255, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993722, Length=259, Percent_Identity=26.6409266409266, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=214, Percent_Identity=37.3831775700935, Blast_Score=135, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6322985, Length=294, Percent_Identity=31.2925170068027, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550503, Length=266, Percent_Identity=27.8195488721804, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24762344, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24762346, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24762348, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130305, Length=265, Percent_Identity=29.811320754717, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24652747, Length=240, Percent_Identity=32.0833333333333, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45551084, Length=240, Percent_Identity=32.0833333333333, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922828, Length=203, Percent_Identity=31.5270935960591, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24762342, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29210; Mature: 29078

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQRPNTLLGECIAEFIGTGLLIFFGVGCVAALVLAGANFGQWEISITWGLGVAIAIYV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEE
TGGISGAHLNPAVTLALMTFCGFDKRKVVPYIIAQVAGAFCFAALVYFLYGGLFTQWEVT
ECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HNVVRGSVESLGSAGIFSTYPNALLSNMQAFAVELVITAVLMLGIMALGDNNNGAPKGFA
HHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AALLIGILIAVIGASLGPLTGFAMNPARDFGPKLFAFLAGWGDVAMTGGRDNPYFWVPIL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEHH
GPIVGAQLGTALYVKVLAPCVPGNRVAATEQAAQTAKEEAAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIQRPNTLLGECIAEFIGTGLLIFFGVGCVAALVLAGANFGQWEISITWGLGVAIAIYV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEE
TGGISGAHLNPAVTLALMTFCGFDKRKVVPYIIAQVAGAFCFAALVYFLYGGLFTQWEVT
ECCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HNVVRGSVESLGSAGIFSTYPNALLSNMQAFAVELVITAVLMLGIMALGDNNNGAPKGFA
HHHHHHHHHHHCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
AALLIGILIAVIGASLGPLTGFAMNPARDFGPKLFAFLAGWGDVAMTGGRDNPYFWVPIL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEHH
GPIVGAQLGTALYVKVLAPCVPGNRVAATEQAAQTAKEEAAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]