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The map label for this gene is glpK [H]

Identifier: 145299636

GI number: 145299636

Start: 2908853

End: 2910355

Strand: Direct

Name: glpK [H]

Synonym: ASA_2710

Alternate gene names: 145299636

Gene position: 2908853-2910355 (Clockwise)

Preceding gene: 145299635

Following gene: 145299638

Centisome position: 61.86

GC content: 61.01

Gene sequence:

>1503_bases
ATGTCTGCTGAAAAAAAATATGTCGTCGCTCTGGACCAGGGTACTACCTCTTCCCGCGCCATCGTGTTTGACCAGGATGC
CAATATCGTCGGCACCTCCCAGCGCGAATTCACCCAGCACTACCCGAAAGCGGGCTGGGTAGAGCACGACCCCATGGAGA
TCTGGGCGACCCAGTCCTCCGTCTTCACCGAAGTACTGGCCAAGACTGGCCTGCGCAGCGAAGAGATTGCCGCCATCGGC
ATCACCAACCAGCGTGAAACCACAGTGGTATGGGAAAAGGCCACCGGCAAACCCATCTACAACGCCATTGTCTGGCAGTG
CCGCCGCACCGCAGCCATCTGCGAAGAGCTCAAGGCCCGTGGCCTGGAAGAGTATGTGCGCGAGAACACCGGTCTGGTGC
TGGATGCCTACTTCTCCGGCACCAAGGTGAAGTGGATCCTCGACAACGTCGAAGGGGCCCGCGAGAAGGCGATGAACGGC
GAACTGCTGTTCGGCACCATAGACACCTGGCTGGTGTGGAAGATGACCAATGGCGAAGTGCACGTCACTGACCCGACCAA
CGCTTCCCGTACCATGCTCTACAACATCCGCGACCTGAAGTGGGACGAGAAGATGCTGGACGAGCTGGGCATTCCCTTGT
CCATGCTGCCAGAGGTCAAACCCTCTTCCGAAGTGTATGGCTACACCACCCGTGGCGGCGGCTCCCGCATCCCCATCGCC
GGCATTGCCGGTGACCAGCAGTCTGCCCTTTTCGGCCAGCTCTGCTTCGAAAAAGGGATGGCCAAGAACACCTACGGTAC
CGGCTGCTTCTTGCTGATGAACACGGGCACAGAGCCGGTTCGCTCCAGCAACGGCCTGCTGACCACGGTCGCCATCGGCC
CGAAAGGGGAAGTGAACTACGCGCTGGAAGGGGCTGTCTTTATGGGCGGCGCTACCGTGCAGTGGCTGCGTGACGAGCTG
AAACTCATCCACGATGCCCGCGATACCGACTACTTCGCCTCCAAGGTGGGTGACACCAACGGCGTCTATCTGGTGCCGGC
CTTCGTCGGCCTGGGCGCACCCTACTGGGATCCCTATGCTCGCGGCACCATGGTCGGCCTGACCCGTGGCGCCAACCGCA
ACCACATCATTCGTGCCGCGCTGGAATCCATCGCCTACCAGAGCCGTGATGTGCTGGATGCCATGCAGCAGGACTCCGGC
ATCAAGCTGGCCGCCCTCAAGGTAGACGGCGGCGCGGTTGCCAACGACTTCCTGATGCAGTTCCAGTCCGACATGATGCA
CACCCCTGTGGTGCGTCCGACCCGTATCGAGACCACCGCCATGGGCGCCGCCTTCCTGGCCGGTCTGGCCGTCGGCTTCT
GGAAGAGCTCTGACGAGCTGGAAGACAAGTTCAGCGTGGATCGTGAATTCATCCCGCAGATGAGCCACGAAGAGCGCAAC
AAGCTCTACCACGGCTGGCAGAAAGCGGTTGAGCGTTCACGCCGCTGGGCCGAAGAGGATTAA

Upstream 100 bases:

>100_bases
CACCGAGCAAGCTGCCCAAACCGCCAAAGAAGAGGCCGCAGCATAAGAGCGGCCCTCCCCAACGTGTAACAACGTGGAAA
ATAAAAACGTGGAGAACATC

Downstream 100 bases:

>100_bases
TCGGGCAACCGACATCTTTCCGAGATGAACAAACAAAGAGGCCGACAGCGATGTCGGCCTCTTCTTTGAGCTCTTTATCC
ACTCCGGCCATTGGCAACGT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSAEKKYVVALDQGTTSSRAIVFDQDANIVGTSQREFTQHYPKAGWVEHDPMEIWATQSSVFTEVLAKTGLRSEEIAAIG
ITNQRETTVVWEKATGKPIYNAIVWQCRRTAAICEELKARGLEEYVRENTGLVLDAYFSGTKVKWILDNVEGAREKAMNG
ELLFGTIDTWLVWKMTNGEVHVTDPTNASRTMLYNIRDLKWDEKMLDELGIPLSMLPEVKPSSEVYGYTTRGGGSRIPIA
GIAGDQQSALFGQLCFEKGMAKNTYGTGCFLLMNTGTEPVRSSNGLLTTVAIGPKGEVNYALEGAVFMGGATVQWLRDEL
KLIHDARDTDYFASKVGDTNGVYLVPAFVGLGAPYWDPYARGTMVGLTRGANRNHIIRAALESIAYQSRDVLDAMQQDSG
IKLAALKVDGGAVANDFLMQFQSDMMHTPVVRPTRIETTAMGAAFLAGLAVGFWKSSDELEDKFSVDREFIPQMSHEERN
KLYHGWQKAVERSRRWAEED

Sequences:

>Translated_500_residues
MSAEKKYVVALDQGTTSSRAIVFDQDANIVGTSQREFTQHYPKAGWVEHDPMEIWATQSSVFTEVLAKTGLRSEEIAAIG
ITNQRETTVVWEKATGKPIYNAIVWQCRRTAAICEELKARGLEEYVRENTGLVLDAYFSGTKVKWILDNVEGAREKAMNG
ELLFGTIDTWLVWKMTNGEVHVTDPTNASRTMLYNIRDLKWDEKMLDELGIPLSMLPEVKPSSEVYGYTTRGGGSRIPIA
GIAGDQQSALFGQLCFEKGMAKNTYGTGCFLLMNTGTEPVRSSNGLLTTVAIGPKGEVNYALEGAVFMGGATVQWLRDEL
KLIHDARDTDYFASKVGDTNGVYLVPAFVGLGAPYWDPYARGTMVGLTRGANRNHIIRAALESIAYQSRDVLDAMQQDSG
IKLAALKVDGGAVANDFLMQFQSDMMHTPVVRPTRIETTAMGAAFLAGLAVGFWKSSDELEDKFSVDREFIPQMSHEERN
KLYHGWQKAVERSRRWAEED
>Mature_499_residues
SAEKKYVVALDQGTTSSRAIVFDQDANIVGTSQREFTQHYPKAGWVEHDPMEIWATQSSVFTEVLAKTGLRSEEIAAIGI
TNQRETTVVWEKATGKPIYNAIVWQCRRTAAICEELKARGLEEYVRENTGLVLDAYFSGTKVKWILDNVEGAREKAMNGE
LLFGTIDTWLVWKMTNGEVHVTDPTNASRTMLYNIRDLKWDEKMLDELGIPLSMLPEVKPSSEVYGYTTRGGGSRIPIAG
IAGDQQSALFGQLCFEKGMAKNTYGTGCFLLMNTGTEPVRSSNGLLTTVAIGPKGEVNYALEGAVFMGGATVQWLRDELK
LIHDARDTDYFASKVGDTNGVYLVPAFVGLGAPYWDPYARGTMVGLTRGANRNHIIRAALESIAYQSRDVLDAMQQDSGI
KLAALKVDGGAVANDFLMQFQSDMMHTPVVRPTRIETTAMGAAFLAGLAVGFWKSSDELEDKFSVDREFIPQMSHEERNK
LYHGWQKAVERSRRWAEED

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=514, Percent_Identity=48.2490272373541, Blast_Score=463, Evalue=1e-130,
Organism=Homo sapiens, GI4504007, Length=513, Percent_Identity=48.3430799220273, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI42794763, Length=519, Percent_Identity=47.7842003853565, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=46.6403162055336, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=34.251968503937, Blast_Score=311, Evalue=9e-85,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=73.1462925851703, Blast_Score=770, Evalue=0.0,
Organism=Escherichia coli, GI1789138, Length=487, Percent_Identity=22.7926078028747, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787789, Length=523, Percent_Identity=25.8126195028681, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790005, Length=511, Percent_Identity=23.4833659491194, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789987, Length=502, Percent_Identity=22.9083665338645, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535599, Length=502, Percent_Identity=42.4302788844622, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=25.5278310940499, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=265, Percent_Identity=31.6981132075472, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321755, Length=558, Percent_Identity=36.0215053763441, Blast_Score=303, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=45.6692913385827, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=40.9181636726547, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=40.9181636726547, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=40.9181636726547, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=40.9181636726547, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=40.9181636726547, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=46.7415730337079, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=32.6335877862595, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=33.4012219959267, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=29.0697674418605, Blast_Score=215, Evalue=5e-56,
Organism=Drosophila melanogaster, GI21357867, Length=533, Percent_Identity=28.5178236397749, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24656372, Length=368, Percent_Identity=30.7065217391304, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45552909, Length=296, Percent_Identity=28.3783783783784, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55493; Mature: 55362

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEKKYVVALDQGTTSSRAIVFDQDANIVGTSQREFTQHYPKAGWVEHDPMEIWATQSS
CCCCCEEEEEEECCCCCCEEEEEECCCCEEECCHHHHHHHCCCCCCCCCCCCEEEECHHH
VFTEVLAKTGLRSEEIAAIGITNQRETTVVWEKATGKPIYNAIVWQCRRTAAICEELKAR
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
GLEEYVRENTGLVLDAYFSGTKVKWILDNVEGAREKAMNGELLFGTIDTWLVWKMTNGEV
CHHHHHHCCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEEECEEEEEEEECCEE
HVTDPTNASRTMLYNIRDLKWDEKMLDELGIPLSMLPEVKPSSEVYGYTTRGGGSRIPIA
EEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCCCCCEEE
GIAGDQQSALFGQLCFEKGMAKNTYGTGCFLLMNTGTEPVRSSNGLLTTVAIGPKGEVNY
EECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEE
ALEGAVFMGGATVQWLRDELKLIHDARDTDYFASKVGDTNGVYLVPAFVGLGAPYWDPYA
EEECEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEHHHHCCCCCCCCHH
RGTMVGLTRGANRNHIIRAALESIAYQSRDVLDAMQQDSGIKLAALKVDGGAVANDFLMQ
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
FQSDMMHTPVVRPTRIETTAMGAAFLAGLAVGFWKSSDELEDKFSVDREFIPQMSHEERN
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCHHHHH
KLYHGWQKAVERSRRWAEED
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAEKKYVVALDQGTTSSRAIVFDQDANIVGTSQREFTQHYPKAGWVEHDPMEIWATQSS
CCCCEEEEEEECCCCCCEEEEEECCCCEEECCHHHHHHHCCCCCCCCCCCCEEEECHHH
VFTEVLAKTGLRSEEIAAIGITNQRETTVVWEKATGKPIYNAIVWQCRRTAAICEELKAR
HHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
GLEEYVRENTGLVLDAYFSGTKVKWILDNVEGAREKAMNGELLFGTIDTWLVWKMTNGEV
CHHHHHHCCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEEECEEEEEEEECCEE
HVTDPTNASRTMLYNIRDLKWDEKMLDELGIPLSMLPEVKPSSEVYGYTTRGGGSRIPIA
EEECCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCCCCCCCCEEEEEECCCCCCCEEE
GIAGDQQSALFGQLCFEKGMAKNTYGTGCFLLMNTGTEPVRSSNGLLTTVAIGPKGEVNY
EECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCEEE
ALEGAVFMGGATVQWLRDELKLIHDARDTDYFASKVGDTNGVYLVPAFVGLGAPYWDPYA
EEECEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEHHHHCCCCCCCCHH
RGTMVGLTRGANRNHIIRAALESIAYQSRDVLDAMQQDSGIKLAALKVDGGAVANDFLMQ
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
FQSDMMHTPVVRPTRIETTAMGAAFLAGLAVGFWKSSDELEDKFSVDREFIPQMSHEERN
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCCCHHHHH
KLYHGWQKAVERSRRWAEED
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA