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The map label for this gene is nrfD [H]

Identifier: 145299280

GI number: 145299280

Start: 2464264

End: 2465214

Strand: Direct

Name: nrfD [H]

Synonym: ASA_2324

Alternate gene names: 145299280

Gene position: 2464264-2465214 (Clockwise)

Preceding gene: 145299279

Following gene: 145299281

Centisome position: 52.4

GC content: 58.89

Gene sequence:

>951_bases
ATGTGGCATGACGCATTTCATTTTCCTTCCCTTGTCTGGGACTGGCCCATCGCCATCTACCTCTTTCTGCTCGGCATCTC
CGCCGGGGCCACCAGCCTCTCGGTGTTGTTGCGCCGCAAAGGGGCTGGCAGCGAGAGCGGCATCATCAAGGCGACCGCCA
TTCTGGCACCGCTAAGCGTGATATTGGGGCTCTTGATCCTCATCTTCCACCTGGCCCGTCCCTGGACCTTCTGGAAGCTG
ATGTTCCACTACCAGTTCGACTCAGTGATGTCGATGGGGGTCATGCTGTTCCAGCTCTATATGGCGGTGCTGTTTGCCTG
GCTGGCGGTGGTATTTCGCAGCGAGATCGAGGAACTGCGCAGGCACCTGTTCAAGGAGACGTTCGCCTTCGTGGATAGCA
CGATAGTCCTGCTGGCCAGATTCGAACGTCTGCTGGCGCCGCTGCTGTTGCTGCTGGCCGTGCTGCTCGGCGCCTACACC
GGCTTTTTGCTCTCTGCGCTCAAGACTTATCCGCTGCTCAACAACCCTGTGCTGCCTGTGCTGTTTCTGGTCTCCGGCAT
CAGTTCAGGCATTGCCTCCACCATCTTGCTGGCGGTGACTCTCTTCAATGAGGATCACCACAGCCAGGGAGTGAGCTTTG
TGCACGGCTTCGAGCGACCCGTGCTGGCGGTAGAGGTGCTGCTGCTGGTCTGCTTCTTTACCGGCCTCTACTTTGGTGGT
GGCCAGAAAGAGGTGGCCATGTGGGCCGCCATCGGCAGCGGCTTCTGGGCCCAGGTGTTCTGGCTTGGTGTGGTGGGTTT
CGGCATGCTGCTGCCCCAGTTGCTCTCCCTGCTACCAGCACAAATGCGCCAGCGCAACGGTCATCTGGTGCTGGTGTGCA
GCCTGACCCTGATGGGGATACTGCTGCTGCGTTACTTCGTGCTCTATGCAGGTCAAATGACTGTTGTATAA

Upstream 100 bases:

>100_bases
CAAGCAGGCGCTCGGCACCTCGCCCAAGATGTACCGTGTGCCCAAAGGGGAGATAACCTCATGACCATGCAAGCGGCAGT
AACCCAAGGAGGGAATAACC

Downstream 100 bases:

>100_bases
AGGGAGATACATGATGGGTAGCAAAAGCGACCATACGACGTCGGCTTGCAGCATGACCCTGATGGGCAGGTTGCTGACTG
ATGCTGCGTCATCCTCGTAT

Product: formate-dependent nitrite reductase, membrane subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MWHDAFHFPSLVWDWPIAIYLFLLGISAGATSLSVLLRRKGAGSESGIIKATAILAPLSVILGLLILIFHLARPWTFWKL
MFHYQFDSVMSMGVMLFQLYMAVLFAWLAVVFRSEIEELRRHLFKETFAFVDSTIVLLARFERLLAPLLLLLAVLLGAYT
GFLLSALKTYPLLNNPVLPVLFLVSGISSGIASTILLAVTLFNEDHHSQGVSFVHGFERPVLAVEVLLLVCFFTGLYFGG
GQKEVAMWAAIGSGFWAQVFWLGVVGFGMLLPQLLSLLPAQMRQRNGHLVLVCSLTLMGILLLRYFVLYAGQMTVV

Sequences:

>Translated_316_residues
MWHDAFHFPSLVWDWPIAIYLFLLGISAGATSLSVLLRRKGAGSESGIIKATAILAPLSVILGLLILIFHLARPWTFWKL
MFHYQFDSVMSMGVMLFQLYMAVLFAWLAVVFRSEIEELRRHLFKETFAFVDSTIVLLARFERLLAPLLLLLAVLLGAYT
GFLLSALKTYPLLNNPVLPVLFLVSGISSGIASTILLAVTLFNEDHHSQGVSFVHGFERPVLAVEVLLLVCFFTGLYFGG
GQKEVAMWAAIGSGFWAQVFWLGVVGFGMLLPQLLSLLPAQMRQRNGHLVLVCSLTLMGILLLRYFVLYAGQMTVV
>Mature_316_residues
MWHDAFHFPSLVWDWPIAIYLFLLGISAGATSLSVLLRRKGAGSESGIIKATAILAPLSVILGLLILIFHLARPWTFWKL
MFHYQFDSVMSMGVMLFQLYMAVLFAWLAVVFRSEIEELRRHLFKETFAFVDSTIVLLARFERLLAPLLLLLAVLLGAYT
GFLLSALKTYPLLNNPVLPVLFLVSGISSGIASTILLAVTLFNEDHHSQGVSFVHGFERPVLAVEVLLLVCFFTGLYFGG
GQKEVAMWAAIGSGFWAQVFWLGVVGFGMLLPQLLSLLPAQMRQRNGHLVLVCSLTLMGILLLRYFVLYAGQMTVV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=316, Percent_Identity=55.379746835443, Blast_Score=312, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35199; Mature: 35199

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHDAFHFPSLVWDWPIAIYLFLLGISAGATSLSVLLRRKGAGSESGIIKATAILAPLSV
CCCCCHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ILGLLILIFHLARPWTFWKLMFHYQFDSVMSMGVMLFQLYMAVLFAWLAVVFRSEIEELR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLFKETFAFVDSTIVLLARFERLLAPLLLLLAVLLGAYTGFLLSALKTYPLLNNPVLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLVSGISSGIASTILLAVTLFNEDHHSQGVSFVHGFERPVLAVEVLLLVCFFTGLYFGG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GQKEVAMWAAIGSGFWAQVFWLGVVGFGMLLPQLLSLLPAQMRQRNGHLVLVCSLTLMGI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
LLLRYFVLYAGQMTVV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MWHDAFHFPSLVWDWPIAIYLFLLGISAGATSLSVLLRRKGAGSESGIIKATAILAPLSV
CCCCCHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
ILGLLILIFHLARPWTFWKLMFHYQFDSVMSMGVMLFQLYMAVLFAWLAVVFRSEIEELR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLFKETFAFVDSTIVLLARFERLLAPLLLLLAVLLGAYTGFLLSALKTYPLLNNPVLPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLVSGISSGIASTILLAVTLFNEDHHSQGVSFVHGFERPVLAVEVLLLVCFFTGLYFGG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GQKEVAMWAAIGSGFWAQVFWLGVVGFGMLLPQLLSLLPAQMRQRNGHLVLVCSLTLMGI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
LLLRYFVLYAGQMTVV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]