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The map label for this gene is nrfC [H]

Identifier: 145299279

GI number: 145299279

Start: 2463547

End: 2464227

Strand: Direct

Name: nrfC [H]

Synonym: ASA_2323

Alternate gene names: 145299279

Gene position: 2463547-2464227 (Clockwise)

Preceding gene: 145299278

Following gene: 145299280

Centisome position: 52.39

GC content: 62.26

Gene sequence:

>681_bases
ATGAGCTGCTCTCGTCGTGACTTTATGGCGGGCATGAGCGCGGGGGCGCTGATCATGATGGCGGGCCCCGTGCGTGCCAG
CGCGGCCATCCTCTCCCACAGCCAGACCATAGACGGGGTACGCTACGGCATGATCCATGACGAGGCGGCCTGCATCGGCT
GTACCGCCTGCATGGATGCCTGCCGGGAGGTGAATCAGGTACCGGAAGGGGTCTCGCGGCTCGAGATCATCCGCTCCGAC
CCCATAGGCACATTCCCCGATGCCGAGTACCACTTCTTTCGCAAGTCCTGCCAGCACTGCGACAACGCCCCCTGCGTGCA
TGTCTGCCCGACCGGTGCCTCCCATATCAGAGCGGAAGATGGCATCGTCGATGTGGACCCGGACCTCTGTGTCGGTTGCA
TGTATTGTCTGGCCGCCTGTCCCTATCAGGTGCGCTTTATCAACCCTGTGACCAAGGTCGCCGACAAGTGTGATTTTTGC
CGCAAGACCAACCTGGCCCAGGGCAAGGAGCCGGCCTGCGTGGCCTCCTGCCCCACCAGGGCGCTGGTGTTCGGCAATCT
GGATGATCCCGATAGCCCCATCGCCAAACGGCTGGTGAAGGAGACCACCTATCGCTACAAGCAGGCGCTCGGCACCTCGC
CCAAGATGTACCGTGTGCCCAAAGGGGAGATAACCTCATGA

Upstream 100 bases:

>100_bases
TACCGGAAAAGTCCCGCATCAAGCTCTGCGTCGACTGCCATAGCAAGCAGCATGAGGCCAAGGCAACCGCTCAGCAGGCG
AGCGTGAGCAAGGAGGCACC

Downstream 100 bases:

>100_bases
CCATGCAAGCGGCAGTAACCCAAGGAGGGAATAACCATGTGGCATGACGCATTTCATTTTCCTTCCCTTGTCTGGGACTG
GCCCATCGCCATCTACCTCT

Product: formate-dependent nitrite reductase, 4Fe4S subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSCSRRDFMAGMSAGALIMMAGPVRASAAILSHSQTIDGVRYGMIHDEAACIGCTACMDACREVNQVPEGVSRLEIIRSD
PIGTFPDAEYHFFRKSCQHCDNAPCVHVCPTGASHIRAEDGIVDVDPDLCVGCMYCLAACPYQVRFINPVTKVADKCDFC
RKTNLAQGKEPACVASCPTRALVFGNLDDPDSPIAKRLVKETTYRYKQALGTSPKMYRVPKGEITS

Sequences:

>Translated_226_residues
MSCSRRDFMAGMSAGALIMMAGPVRASAAILSHSQTIDGVRYGMIHDEAACIGCTACMDACREVNQVPEGVSRLEIIRSD
PIGTFPDAEYHFFRKSCQHCDNAPCVHVCPTGASHIRAEDGIVDVDPDLCVGCMYCLAACPYQVRFINPVTKVADKCDFC
RKTNLAQGKEPACVASCPTRALVFGNLDDPDSPIAKRLVKETTYRYKQALGTSPKMYRVPKGEITS
>Mature_225_residues
SCSRRDFMAGMSAGALIMMAGPVRASAAILSHSQTIDGVRYGMIHDEAACIGCTACMDACREVNQVPEGVSRLEIIRSDP
IGTFPDAEYHFFRKSCQHCDNAPCVHVCPTGASHIRAEDGIVDVDPDLCVGCMYCLAACPYQVRFINPVTKVADKCDFCR
KTNLAQGKEPACVASCPTRALVFGNLDDPDSPIAKRLVKETTYRYKQALGTSPKMYRVPKGEITS

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=228, Percent_Identity=66.2280701754386, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI226510944, Length=218, Percent_Identity=48.1651376146789, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1789370, Length=255, Percent_Identity=31.7647058823529, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787872, Length=157, Percent_Identity=38.8535031847134, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787122, Length=157, Percent_Identity=38.8535031847134, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790326, Length=178, Percent_Identity=30.3370786516854, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787749, Length=184, Percent_Identity=29.3478260869565, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787740, Length=114, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787478, Length=103, Percent_Identity=34.9514563106796, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789067, Length=164, Percent_Identity=33.5365853658537, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082180, Length=138, Percent_Identity=36.231884057971, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788811, Length=149, Percent_Identity=32.8859060402685, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=150, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=27.741935483871, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367245, Length=150, Percent_Identity=34, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789079, Length=195, Percent_Identity=28.7179487179487, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24553; Mature: 24421

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
11.5 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
11.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCSRRDFMAGMSAGALIMMAGPVRASAAILSHSQTIDGVRYGMIHDEAACIGCTACMDA
CCCCHHHHHHCCCCCEEEEEECCHHHHHHHHHCCHHHCCHHCCCCCCCHHHHHHHHHHHH
CREVNQVPEGVSRLEIIRSDPIGTFPDAEYHFFRKSCQHCDNAPCVHVCPTGASHIRAED
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHEECCC
GIVDVDPDLCVGCMYCLAACPYQVRFINPVTKVADKCDFCRKTNLAQGKEPACVASCPTR
CEEECCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCC
ALVFGNLDDPDSPIAKRLVKETTYRYKQALGTSPKMYRVPKGEITS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SCSRRDFMAGMSAGALIMMAGPVRASAAILSHSQTIDGVRYGMIHDEAACIGCTACMDA
CCCHHHHHHCCCCCEEEEEECCHHHHHHHHHCCHHHCCHHCCCCCCCHHHHHHHHHHHH
CREVNQVPEGVSRLEIIRSDPIGTFPDAEYHFFRKSCQHCDNAPCVHVCPTGASHIRAED
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHEECCC
GIVDVDPDLCVGCMYCLAACPYQVRFINPVTKVADKCDFCRKTNLAQGKEPACVASCPTR
CEEECCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCC
ALVFGNLDDPDSPIAKRLVKETTYRYKQALGTSPKMYRVPKGEITS
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]