Click here to switch to the map view.

The map label for this gene is nrfB [H]

Identifier: 145299278

GI number: 145299278

Start: 2462954

End: 2463550

Strand: Direct

Name: nrfB [H]

Synonym: ASA_2322

Alternate gene names: 145299278

Gene position: 2462954-2463550 (Clockwise)

Preceding gene: 145299277

Following gene: 145299279

Centisome position: 52.38

GC content: 62.14

Gene sequence:

>597_bases
ATGGGTGATTTAACAATGGATTTTTTACGACTGATGCTGATGGCAGCCATGCTGCTCACCAGCCTGCAGGGACAGGCGGG
CGACGCCGCGATCGCGCCCGCTGCCATGCCCGCGACGCATGAAGTTGAGTTTTTACGCAGCCCCGACAAGGCCTGCACCG
ACTGCCACAAGGAGCAGGCAAGCGGCATGCACGGCAAACATGGCCAGACAACCAACCCCAACAACCTGGCGCCGGTCACC
TGCACCAACTGCCACGGCCAGCCCTCCCCCCAGCACAGGGAAGGGGTCAAGGATGTGATGCGGTTCAGTGACAGCTTCAA
CCCTCCGCAAGGCAAGGAAGCTTACCCCATCGCCGAGCAGAACGGGGTCTGCATGAGCTGCCACGAACCCAAGCCATTGC
GCGAGGCGCTTTGGGCCCACGACGTGCACGCCACCAGGCTCACCTGCACCAGCTGTCATGCACTGCACCCGGCCAAGGAG
CCCATGGTGGGCATACCGGAAAAGTCCCGCATCAAGCTCTGCGTCGACTGCCATAGCAAGCAGCATGAGGCCAAGGCAAC
CGCTCAGCAGGCGAGCGTGAGCAAGGAGGCACCATGA

Upstream 100 bases:

>100_bases
CCGCCTGGGATGCCGAGGCCAAGGCGGCCGGACGGCTGGCACAGTAACGGCGACTGCGGGCCCGGCCCCAAGTCGGGCCC
GCAGCTGATGGAGGCTTCCT

Downstream 100 bases:

>100_bases
GCTGCTCTCGTCGTGACTTTATGGCGGGCATGAGCGCGGGGGCGCTGATCATGATGGCGGGCCCCGTGCGTGCCAGCGCG
GCCATCCTCTCCCACAGCCA

Product: formate-dependent nitrite reductase, penta-heme cytochrome c

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MGDLTMDFLRLMLMAAMLLTSLQGQAGDAAIAPAAMPATHEVEFLRSPDKACTDCHKEQASGMHGKHGQTTNPNNLAPVT
CTNCHGQPSPQHREGVKDVMRFSDSFNPPQGKEAYPIAEQNGVCMSCHEPKPLREALWAHDVHATRLTCTSCHALHPAKE
PMVGIPEKSRIKLCVDCHSKQHEAKATAQQASVSKEAP

Sequences:

>Translated_198_residues
MGDLTMDFLRLMLMAAMLLTSLQGQAGDAAIAPAAMPATHEVEFLRSPDKACTDCHKEQASGMHGKHGQTTNPNNLAPVT
CTNCHGQPSPQHREGVKDVMRFSDSFNPPQGKEAYPIAEQNGVCMSCHEPKPLREALWAHDVHATRLTCTSCHALHPAKE
PMVGIPEKSRIKLCVDCHSKQHEAKATAQQASVSKEAP
>Mature_197_residues
GDLTMDFLRLMLMAAMLLTSLQGQAGDAAIAPAAMPATHEVEFLRSPDKACTDCHKEQASGMHGKHGQTTNPNNLAPVTC
TNCHGQPSPQHREGVKDVMRFSDSFNPPQGKEAYPIAEQNGVCMSCHEPKPLREALWAHDVHATRLTCTSCHALHPAKEP
MVGIPEKSRIKLCVDCHSKQHEAKATAQQASVSKEAP

Specific function: Plays a role in nitrite reduction [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082366, Length=178, Percent_Identity=52.8089887640449, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017564
- InterPro:   IPR011031 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21468; Mature: 21336

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
9.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLTMDFLRLMLMAAMLLTSLQGQAGDAAIAPAAMPATHEVEFLRSPDKACTDCHKEQA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHH
SGMHGKHGQTTNPNNLAPVTCTNCHGQPSPQHREGVKDVMRFSDSFNPPQGKEAYPIAEQ
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NGVCMSCHEPKPLREALWAHDVHATRLTCTSCHALHPAKEPMVGIPEKSRIKLCVDCHSK
CCEEEECCCCCHHHHHHHHHCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC
QHEAKATAQQASVSKEAP
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GDLTMDFLRLMLMAAMLLTSLQGQAGDAAIAPAAMPATHEVEFLRSPDKACTDCHKEQA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHH
SGMHGKHGQTTNPNNLAPVTCTNCHGQPSPQHREGVKDVMRFSDSFNPPQGKEAYPIAEQ
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
NGVCMSCHEPKPLREALWAHDVHATRLTCTSCHALHPAKEPMVGIPEKSRIKLCVDCHSK
CCEEEECCCCCHHHHHHHHHCCHHHHEEHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCC
QHEAKATAQQASVSKEAP
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]