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The map label for this gene is nrfA [H]

Identifier: 145299277

GI number: 145299277

Start: 2461458

End: 2462900

Strand: Direct

Name: nrfA [H]

Synonym: ASA_2321

Alternate gene names: 145299277

Gene position: 2461458-2462900 (Clockwise)

Preceding gene: 145299276

Following gene: 145299278

Centisome position: 52.34

GC content: 61.54

Gene sequence:

>1443_bases
ATGATCGTGAGTTCACACAACAGACTGACCAGACTGGGCCTCGCCGCCCTGCTGCTTACCACCTGCTTTGGCGCCAGCGG
CTTGGCACAGGCCGATGAGGCAACCCCCAAGGGCAAGATAGAGGCACGCAGCGAGCTGTTCGCCAAGGATCACGTCGACC
AGTTCAGCAGCTGGAAAGGCACCAGCGAGAGCGCGACGCGCACCGATGCCCTGGCCGAAGATCCGCAGATGGTGGTGCTC
TGGGGGGGTTATCCCTTCTCCAAGGATTACAACAAGCCCCGCGGCCATTTCTATGCCCTGACCGACGTACGGGAAACCCT
GCGCACCGGCGCTCCCAAGACCGCCGAGGATGGCCCCCTGCCCATGGCCTGCTGGAGCTGCAAAGGCCCGGATGTGGCCC
GCGTCATCGATGAAAAAGGCGAAGATGGCTACTTCAAGGGCATGTGGGCCAAGGGTGGGTCGGAAATAGTCAACACCATA
GGCTGCGCCGACTGCCACGACACGGCGTCGAAAGAGTTCAAGGAGGGCAAACCCGCCCTGCACCTCTCCCGTCCCTATGC
CGACCGCGCCATGACCGCCATCGGCAAGCCGTTCAAAGAGCAGGGACGTTTCGATCAGCAATCCCAGGTGTGTGGCCAGT
GTCACGTGGAGTACTACTTCAGCGGCCCCACCAAGGCGGTGAAATTCCCCTGGGACAAGGGCACCACGGTTGAGCAGATG
GAGCAGTACTACGACGAGATTGGTTTCGCCGACTGGACCCACGCCCTCTCCAAGACCCCCATGCTCAAGGCCCAGCACCC
TGAATACGAGACCTGGCGCGCAGGCATTCACGGCCAGAACAACGTCGCCTGTGTCGACTGTCACATGCCCAAGGTGCAAA
ACGAGCAGGGCAAGGTCTACACGGATCACAAGGTCGGCAACCCCTTCGATCGCTTCGAGCAGACCTGCCGCAACTGCCAT
ACCCAGGGCAAGGAGATGCTGCAAGGCATAGTCCAGCAGCGCAAGGATGCGGTGACCGAGATCAAGTTGAAGGTCGATAA
GCAGCTGGTGCACGCCCACTTCGAGGCCAAGGCGGCCTGGGATGCAGGCGCCACCGAGGCGCAGATGAAAGACATACTGC
AAGACATCCGTCACGCCCAATGGCGCTGGGACTTCTCCATCGCTTCCCACGGGGTGCAGATGCACGCCCCGGAAGTGGCA
CTGCGCATGCTGGGTACAGCTCTGGATAAAGCCGCCGATGCCCGCACCAAGCTGGCACGCCTCTTGGCGACCAAGGGGAT
AAGTCACGAGATCGCCCTGCCGGACATCTCCACCAAGGAGAAGGCGCAAGCCGCCCTTGGCATGGATATGAAACAGATGA
ACTCAGACAAGGCCCAGTTCCTCAAGACCACAGTTCCCGCCTGGGATGCCGAGGCCAAGGCGGCCGGACGGCTGGCACAG
TAA

Upstream 100 bases:

>100_bases
TAACAATTAATCGGCCTGCCTGCTCCTGCCTAGTGCGGGGGCTGATGCCCACATCCAGGATGGAACGGTCAGGCTTGGCA
ATGAGATCGATTCGGGAGTG

Downstream 100 bases:

>100_bases
CGGCGACTGCGGGCCCGGCCCCAAGTCGGGCCCGCAGCTGATGGAGGCTTCCTATGGGTGATTTAACAATGGATTTTTTA
CGACTGATGCTGATGGCAGC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MIVSSHNRLTRLGLAALLLTTCFGASGLAQADEATPKGKIEARSELFAKDHVDQFSSWKGTSESATRTDALAEDPQMVVL
WGGYPFSKDYNKPRGHFYALTDVRETLRTGAPKTAEDGPLPMACWSCKGPDVARVIDEKGEDGYFKGMWAKGGSEIVNTI
GCADCHDTASKEFKEGKPALHLSRPYADRAMTAIGKPFKEQGRFDQQSQVCGQCHVEYYFSGPTKAVKFPWDKGTTVEQM
EQYYDEIGFADWTHALSKTPMLKAQHPEYETWRAGIHGQNNVACVDCHMPKVQNEQGKVYTDHKVGNPFDRFEQTCRNCH
TQGKEMLQGIVQQRKDAVTEIKLKVDKQLVHAHFEAKAAWDAGATEAQMKDILQDIRHAQWRWDFSIASHGVQMHAPEVA
LRMLGTALDKAADARTKLARLLATKGISHEIALPDISTKEKAQAALGMDMKQMNSDKAQFLKTTVPAWDAEAKAAGRLAQ

Sequences:

>Translated_480_residues
MIVSSHNRLTRLGLAALLLTTCFGASGLAQADEATPKGKIEARSELFAKDHVDQFSSWKGTSESATRTDALAEDPQMVVL
WGGYPFSKDYNKPRGHFYALTDVRETLRTGAPKTAEDGPLPMACWSCKGPDVARVIDEKGEDGYFKGMWAKGGSEIVNTI
GCADCHDTASKEFKEGKPALHLSRPYADRAMTAIGKPFKEQGRFDQQSQVCGQCHVEYYFSGPTKAVKFPWDKGTTVEQM
EQYYDEIGFADWTHALSKTPMLKAQHPEYETWRAGIHGQNNVACVDCHMPKVQNEQGKVYTDHKVGNPFDRFEQTCRNCH
TQGKEMLQGIVQQRKDAVTEIKLKVDKQLVHAHFEAKAAWDAGATEAQMKDILQDIRHAQWRWDFSIASHGVQMHAPEVA
LRMLGTALDKAADARTKLARLLATKGISHEIALPDISTKEKAQAALGMDMKQMNSDKAQFLKTTVPAWDAEAKAAGRLAQ
>Mature_480_residues
MIVSSHNRLTRLGLAALLLTTCFGASGLAQADEATPKGKIEARSELFAKDHVDQFSSWKGTSESATRTDALAEDPQMVVL
WGGYPFSKDYNKPRGHFYALTDVRETLRTGAPKTAEDGPLPMACWSCKGPDVARVIDEKGEDGYFKGMWAKGGSEIVNTI
GCADCHDTASKEFKEGKPALHLSRPYADRAMTAIGKPFKEQGRFDQQSQVCGQCHVEYYFSGPTKAVKFPWDKGTTVEQM
EQYYDEIGFADWTHALSKTPMLKAQHPEYETWRAGIHGQNNVACVDCHMPKVQNEQGKVYTDHKVGNPFDRFEQTCRNCH
TQGKEMLQGIVQQRKDAVTEIKLKVDKQLVHAHFEAKAAWDAGATEAQMKDILQDIRHAQWRWDFSIASHGVQMHAPEVA
LRMLGTALDKAADARTKLARLLATKGISHEIALPDISTKEKAQAALGMDMKQMNSDKAQFLKTTVPAWDAEAKAAGRLAQ

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=478, Percent_Identity=68.6192468619247, Blast_Score=710, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 53278; Mature: 53278

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSSHNRLTRLGLAALLLTTCFGASGLAQADEATPKGKIEARSELFAKDHVDQFSSWKG
CEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TSESATRTDALAEDPQMVVLWGGYPFSKDYNKPRGHFYALTDVRETLRTGAPKTAEDGPL
CCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
PMACWSCKGPDVARVIDEKGEDGYFKGMWAKGGSEIVNTIGCADCHDTASKEFKEGKPAL
CEEEECCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCE
HLSRPYADRAMTAIGKPFKEQGRFDQQSQVCGQCHVEYYFSGPTKAVKFPWDKGTTVEQM
EECCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHEEEEEEECCCCCEEECCCCCCCCHHHH
EQYYDEIGFADWTHALSKTPMLKAQHPEYETWRAGIHGQNNVACVDCHMPKVQNEQGKVY
HHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEE
TDHKVGNPFDRFEQTCRNCHTQGKEMLQGIVQQRKDAVTEIKLKVDKQLVHAHFEAKAAW
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAGATEAQMKDILQDIRHAQWRWDFSIASHGVQMHAPEVALRMLGTALDKAADARTKLAR
CCCCCHHHHHHHHHHHHHHHEEEEHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
LLATKGISHEIALPDISTKEKAQAALGMDMKQMNSDKAQFLKTTVPAWDAEAKAAGRLAQ
HHHHCCCCCEEECCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MIVSSHNRLTRLGLAALLLTTCFGASGLAQADEATPKGKIEARSELFAKDHVDQFSSWKG
CEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TSESATRTDALAEDPQMVVLWGGYPFSKDYNKPRGHFYALTDVRETLRTGAPKTAEDGPL
CCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCCC
PMACWSCKGPDVARVIDEKGEDGYFKGMWAKGGSEIVNTIGCADCHDTASKEFKEGKPAL
CEEEECCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCCCCE
HLSRPYADRAMTAIGKPFKEQGRFDQQSQVCGQCHVEYYFSGPTKAVKFPWDKGTTVEQM
EECCCHHHHHHHHHCCCHHHHCCCCHHHHHHHHEEEEEEECCCCCEEECCCCCCCCHHHH
EQYYDEIGFADWTHALSKTPMLKAQHPEYETWRAGIHGQNNVACVDCHMPKVQNEQGKVY
HHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEE
TDHKVGNPFDRFEQTCRNCHTQGKEMLQGIVQQRKDAVTEIKLKVDKQLVHAHFEAKAAW
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DAGATEAQMKDILQDIRHAQWRWDFSIASHGVQMHAPEVALRMLGTALDKAADARTKLAR
CCCCCHHHHHHHHHHHHHHHEEEEHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
LLATKGISHEIALPDISTKEKAQAALGMDMKQMNSDKAQFLKTTVPAWDAEAKAAGRLAQ
HHHHCCCCCEEECCCCCCHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA