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The map label for this gene is tpiA [H]

Identifier: 145299199

GI number: 145299199

Start: 2366120

End: 2366875

Strand: Direct

Name: tpiA [H]

Synonym: ASA_2232

Alternate gene names: 145299199

Gene position: 2366120-2366875 (Clockwise)

Preceding gene: 145299198

Following gene: 145299200

Centisome position: 50.32

GC content: 60.32

Gene sequence:

>756_bases
ATGAGACAGCACCTGGTAATGGGTAACTGGAAACTGAACGGCACCAAAGCTTCTGTAGAAGCTCTGATCAAAGGACTGAC
CCCGGCTGCTGCCGCTCATCCTTCCGTACAAGTCGCCGTCTGCCCGCCGGTTATCTTTTTGGGTCTGGTTGAGCAGCTGA
CCGCAGGCAGCAAGATTGCCTACGGTGCCCAGAATGCCGACGTTCACGAGTCCGGCGCCTTCACCGGCGAGAACGCCCCC
TCCATGCTCAAAGCCTTCGGTTGCACCTACTCCCTGGTCGGTCACAGCGAGCGCCGTACCCTGCACGCAGAGAACGACGA
CGTGGTAGCCGCCAAGTACGAAGCCATCCAGAAAGCGGGCCTGATCCCCGTATTGTGCATCGGTGAGACCCTGGAGCAGT
TCGAAGCCGGCGTGACCAAGACTGTGGTTGAGACCCAGCTGAAAGCCGTCATCGACCGTTGCGGCATCGCCTCCTTCAGC
AACGCCGTGGTTGCCTATGAGCCGGTATGGGCCATCGGTACCGGCAAGACTGCTACCCCGGAAATCGCTCAATCCGTTCA
CGCTCACATCCGTCAGTACCTGGCCGGTTTTGATGGCGCCGTGGCTGCCAAAGTACAGATCCTGTACGGCGGCTCCGTCA
CTGGTGCAACCGCTGCCGACCTGTTCGGTCAGCCGGACATCGACGGTGGCCTGGTGGGTGGTGCGTCCCTGAAAGTGGAC
GACTTCTCCCAGATCATCGCTGGCGCAGCCAAGTAA

Upstream 100 bases:

>100_bases
AAGCGAAAAAAAATTACGTCATTTGCCGCCGGGTCAGCTATCTTGGGCTCGGTTCAATTCATATAATGTCGCGCAATCAA
ATGCCTAGGAGAGTTACAAG

Downstream 100 bases:

>100_bases
GCTGTTGCCCAAGAGGGGGCTTGGCCCCCTCTTGCATATCGCTTTTCCTATGGCGGCCCCCGCATCCCATACGAAAGCCG
ATATAGTAAAAATGGCTTGT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRQHLVMGNWKLNGTKASVEALIKGLTPAAAAHPSVQVAVCPPVIFLGLVEQLTAGSKIAYGAQNADVHESGAFTGENAP
SMLKAFGCTYSLVGHSERRTLHAENDDVVAAKYEAIQKAGLIPVLCIGETLEQFEAGVTKTVVETQLKAVIDRCGIASFS
NAVVAYEPVWAIGTGKTATPEIAQSVHAHIRQYLAGFDGAVAAKVQILYGGSVTGATAADLFGQPDIDGGLVGGASLKVD
DFSQIIAGAAK

Sequences:

>Translated_251_residues
MRQHLVMGNWKLNGTKASVEALIKGLTPAAAAHPSVQVAVCPPVIFLGLVEQLTAGSKIAYGAQNADVHESGAFTGENAP
SMLKAFGCTYSLVGHSERRTLHAENDDVVAAKYEAIQKAGLIPVLCIGETLEQFEAGVTKTVVETQLKAVIDRCGIASFS
NAVVAYEPVWAIGTGKTATPEIAQSVHAHIRQYLAGFDGAVAAKVQILYGGSVTGATAADLFGQPDIDGGLVGGASLKVD
DFSQIIAGAAK
>Mature_251_residues
MRQHLVMGNWKLNGTKASVEALIKGLTPAAAAHPSVQVAVCPPVIFLGLVEQLTAGSKIAYGAQNADVHESGAFTGENAP
SMLKAFGCTYSLVGHSERRTLHAENDDVVAAKYEAIQKAGLIPVLCIGETLEQFEAGVTKTVVETQLKAVIDRCGIASFS
NAVVAYEPVWAIGTGKTATPEIAQSVHAHIRQYLAGFDGAVAAKVQILYGGSVTGATAADLFGQPDIDGGLVGGASLKVD
DFSQIIAGAAK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=46.7479674796748, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=46.7479674796748, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=51.4056224899598, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=49.3927125506073, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=46.3414634146341, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=47.1544715447154, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=47.1544715447154, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=47.1544715447154, Blast_Score=200, Evalue=9e-52,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 25973; Mature: 25973

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQHLVMGNWKLNGTKASVEALIKGLTPAAAAHPSVQVAVCPPVIFLGLVEQLTAGSKIA
CCCCEEECCEEECCCHHHHHHHHHCCCCCHHCCCCEEEEECHHHHHHHHHHHHCCCCEEE
YGAQNADVHESGAFTGENAPSMLKAFGCTYSLVGHSERRTLHAENDDVVAAKYEAIQKAG
ECCCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCEEECCCCCEEHHHHHHHHHCC
LIPVLCIGETLEQFEAGVTKTVVETQLKAVIDRCGIASFSNAVVAYEPVWAIGTGKTATP
CCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCH
EIAQSVHAHIRQYLAGFDGAVAAKVQILYGGSVTGATAADLFGQPDIDGGLVGGASLKVD
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCEECCCEECHH
DFSQIIAGAAK
HHHHHHHCCCC
>Mature Secondary Structure
MRQHLVMGNWKLNGTKASVEALIKGLTPAAAAHPSVQVAVCPPVIFLGLVEQLTAGSKIA
CCCCEEECCEEECCCHHHHHHHHHCCCCCHHCCCCEEEEECHHHHHHHHHHHHCCCCEEE
YGAQNADVHESGAFTGENAPSMLKAFGCTYSLVGHSERRTLHAENDDVVAAKYEAIQKAG
ECCCCCCCCCCCCCCCCCCHHHHHHHCCCCEECCCCCCCEEECCCCCEEHHHHHHHHHCC
LIPVLCIGETLEQFEAGVTKTVVETQLKAVIDRCGIASFSNAVVAYEPVWAIGTGKTATP
CCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCCCH
EIAQSVHAHIRQYLAGFDGAVAAKVQILYGGSVTGATAADLFGQPDIDGGLVGGASLKVD
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCEECCCEECHH
DFSQIIAGAAK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7639513 [H]