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The map label for this gene is pfkA [H]

Identifier: 145299200

GI number: 145299200

Start: 2367023

End: 2367994

Strand: Direct

Name: pfkA [H]

Synonym: ASA_2233

Alternate gene names: 145299200

Gene position: 2367023-2367994 (Clockwise)

Preceding gene: 145299199

Following gene: 145299201

Centisome position: 50.34

GC content: 59.36

Gene sequence:

>972_bases
ATGACCAAACAACTCAAACGTATTGGTGTTTTGACCAGTGGTGGTGACGCACCGGGTATGAACGCAGCCATTCGCGCCGT
GGTGCGCGCCGGTCTGCATCATGGCCTGGAAGTTTATGGCATCTATGATGGCTACCTTGGTCTGCATCAGGACAGGATCG
TCAAGCTGGAGCGTCACAGCGTCTCCGACGTGGTGAACCGTGGCGGTACCTTCCTGGGTTCTGCCCGTTTCCCCGCCTTC
AAAGATCCGGCCGTGCGTGCCGAAGCGATCGAAAACCTGAAAAAGCACGGCATCGATGCCCTGGTGGTGATCGGCGGTGA
CGGCTCCTACATGGGTGCCAAGAAGCTGACCGAAGAGGGTTTCCCCTGCATCGGCCTGCCGGGCACCATCGACAACGACA
TCGCCGGTACCGACTTCACCATCGGCTTTGATACCGCCCTGAACGTGGTGGTTGACGCCATCGACCGTCTGCGCGACACC
TGCAGCTCCCACCACCGTATTTCAGTGGTCGAGATCATGGGTCGCCACTGCGGCGATCTGGCCATGTCTGCCGCCGTTGC
TGGCGGTGCCGAGTACGTGATAGTACCGGAAGTGGCCTTTGACAAAGAGAAGCTGCTGCAACAGATCAAGGAAGGCGAAG
CCAAAGGCAAAAAACACGCCATCATCACAATCTGTGAACACGTCACCGACGTGAATGCGCTGGCCAAGGACATCGAAGCC
ATGACTGGCCGCGAAACCCGCGCCACCATACTGGGTCATATCCAGCGTGGCGGCTCACCGACTGCACGCGATCGCATCAT
GTCCAGCCGCATGGGTGCCTTCGCCGTCGAACAGCTGCTGGCGGGCCAGGGTGGCCGCTGCGTCGGGATCCAGGGCAACG
AGATGGTGCACCACGACATCATCGACTGCATCGAGAACCTGAAGCGCCCGTTCGATCAGGAGCTGTACGATCTCTCCACC
ACACTGTTCTAA

Upstream 100 bases:

>100_bases
CTATGGCGGCCCCCGCATCCCATACGAAAGCCGATATAGTAAAAATGGCTTGTCTCATTTGTAAAACTTCGTATTCGACA
ACTCAGATCCAGAGGCACTC

Downstream 100 bases:

>100_bases
GCCCCATGGCTTGCATCAGGTTGTTGTGACAAAAACCCTCCGCCGGAGGGTTTTTTAATCCGCCTTGTTTACCGCAGCGT
CAGCAGTAAACAGAGGCATT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKQLKRIGVLTSGGDAPGMNAAIRAVVRAGLHHGLEVYGIYDGYLGLHQDRIVKLERHSVSDVVNRGGTFLGSARFPAF
KDPAVRAEAIENLKKHGIDALVVIGGDGSYMGAKKLTEEGFPCIGLPGTIDNDIAGTDFTIGFDTALNVVVDAIDRLRDT
CSSHHRISVVEIMGRHCGDLAMSAAVAGGAEYVIVPEVAFDKEKLLQQIKEGEAKGKKHAIITICEHVTDVNALAKDIEA
MTGRETRATILGHIQRGGSPTARDRIMSSRMGAFAVEQLLAGQGGRCVGIQGNEMVHHDIIDCIENLKRPFDQELYDLST
TLF

Sequences:

>Translated_323_residues
MTKQLKRIGVLTSGGDAPGMNAAIRAVVRAGLHHGLEVYGIYDGYLGLHQDRIVKLERHSVSDVVNRGGTFLGSARFPAF
KDPAVRAEAIENLKKHGIDALVVIGGDGSYMGAKKLTEEGFPCIGLPGTIDNDIAGTDFTIGFDTALNVVVDAIDRLRDT
CSSHHRISVVEIMGRHCGDLAMSAAVAGGAEYVIVPEVAFDKEKLLQQIKEGEAKGKKHAIITICEHVTDVNALAKDIEA
MTGRETRATILGHIQRGGSPTARDRIMSSRMGAFAVEQLLAGQGGRCVGIQGNEMVHHDIIDCIENLKRPFDQELYDLST
TLF
>Mature_322_residues
TKQLKRIGVLTSGGDAPGMNAAIRAVVRAGLHHGLEVYGIYDGYLGLHQDRIVKLERHSVSDVVNRGGTFLGSARFPAFK
DPAVRAEAIENLKKHGIDALVVIGGDGSYMGAKKLTEEGFPCIGLPGTIDNDIAGTDFTIGFDTALNVVVDAIDRLRDTC
SSHHRISVVEIMGRHCGDLAMSAAVAGGAEYVIVPEVAFDKEKLLQQIKEGEAKGKKHAIITICEHVTDVNALAKDIEAM
TGRETRATILGHIQRGGSPTARDRIMSSRMGAFAVEQLLAGQGGRCVGIQGNEMVHHDIIDCIENLKRPFDQELYDLSTT
LF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=358, Percent_Identity=39.9441340782123, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI11321601, Length=352, Percent_Identity=38.6363636363636, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=39.3854748603352, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=39.3854748603352, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=39.3854748603352, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=39.3854748603352, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=71.7868338557994, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25147584, Length=338, Percent_Identity=37.2781065088757, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=39.8058252427184, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=34.7079037800687, Blast_Score=150, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321679, Length=379, Percent_Identity=36.6754617414248, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=354, Percent_Identity=32.2033898305085, Blast_Score=173, Evalue=4e-44,
Organism=Drosophila melanogaster, GI28573326, Length=342, Percent_Identity=37.1345029239766, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24652337, Length=342, Percent_Identity=36.5497076023392, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17647809, Length=338, Percent_Identity=36.9822485207101, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 34754; Mature: 34623

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQLKRIGVLTSGGDAPGMNAAIRAVVRAGLHHGLEVYGIYDGYLGLHQDRIVKLERHS
CCCHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
VSDVVNRGGTFLGSARFPAFKDPAVRAEAIENLKKHGIDALVVIGGDGSYMGAKKLTEEG
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCC
FPCIGLPGTIDNDIAGTDFTIGFDTALNVVVDAIDRLRDTCSSHHRISVVEIMGRHCGDL
CCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AMSAAVAGGAEYVIVPEVAFDKEKLLQQIKEGEAKGKKHAIITICEHVTDVNALAKDIEA
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
MTGRETRATILGHIQRGGSPTARDRIMSSRMGAFAVEQLLAGQGGRCVGIQGNEMVHHDI
HCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
IDCIENLKRPFDQELYDLSTTLF
HHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKQLKRIGVLTSGGDAPGMNAAIRAVVRAGLHHGLEVYGIYDGYLGLHQDRIVKLERHS
CCHHHHHCCEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
VSDVVNRGGTFLGSARFPAFKDPAVRAEAIENLKKHGIDALVVIGGDGSYMGAKKLTEEG
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCC
FPCIGLPGTIDNDIAGTDFTIGFDTALNVVVDAIDRLRDTCSSHHRISVVEIMGRHCGDL
CCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AMSAAVAGGAEYVIVPEVAFDKEKLLQQIKEGEAKGKKHAIITICEHVTDVNALAKDIEA
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHH
MTGRETRATILGHIQRGGSPTARDRIMSSRMGAFAVEQLLAGQGGRCVGIQGNEMVHHDI
HCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
IDCIENLKRPFDQELYDLSTTLF
HHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA