Click here to switch to the map view.

The map label for this gene is glpK

Identifier: 145296878

GI number: 145296878

Start: 3084518

End: 3086047

Strand: Reverse

Name: glpK

Synonym: cgR_2778

Alternate gene names: 145296878

Gene position: 3086047-3084518 (Counterclockwise)

Preceding gene: 145296879

Following gene: 145296876

Centisome position: 93.12

GC content: 57.25

Gene sequence:

>1530_bases
ATGAGAATCTCAAAGGCCAATGCGTATGTTGCAGCGATTGACCAAGGCACCACTTCCACTCGGTGCATCTTCATTGATGC
CCAAGGAAAAGTGGTGTCTTCTGCTTCTAAGGAGCACCGCCAAATCTTCCCACAACAGGGCTGGGTAGAGCACGATCCTG
AAGAAATTTGGGACAACATCCGATCTGTCGTCAGCCAGGCGATGGTCTCCATTGACATCACCCCACACGAGGTTGCATCG
CTGGGAGTCACCAACCAGCGCGAAACCACCGTGGTGTGGGACAAGCACACCGGCGAACCTGTCTACAACGCAATCGTGTG
GCAAGACACCCGCACCTCTGACATTTGCCTAGAGATCGCGGGCGAAGAAGGCCAAGAAAAGTGGCTTGACCGCACCGGCC
TGCTGATCAACTCCTACCCATCGGGGCCCAAAATCAAGTGGATTCTTGACAACGTTGAGGGAGCTCGCGAACGCGCCGAA
AAGGGCGACCTTTTGTTTGGCACCATGGATACCTGGGTGCTGTGGAACCTGACCGGCGGTGTCCGCGGCGACGACGGTGA
TGATGCCATCCACGTCACCGATGTCACCAACGCATCCCGCACACTATTGATGGATCTCCGCACACAACAGTGGGATCCAG
AACTATGCGAAGCCCTAGACATTCCGATGTCCATGCTCCCTGAGATTCGTCCCTCCGTCGGAGAATTCCGCTCCGTGCGC
CACCGCGGAACCCTAGCCGACGTCCCCATTACTGGCGTGCTCGGCGACCAGCAAGCGGCCCTTTTTGGTCAGGGCGGATT
CCACGAAGGTGCTGCTAAAAATACCTACGGCACCGGCCTCTTCCTGCTGATGAACACCGGCACCTCGTTGAAGATTTCCG
AGCACGGCCTGCTGTCCACCATCGCCTATCAACGGGAAGGATCCGCTCCGGTCTACGCGCTGGAAGGTTCCGTATCCATG
GGCGGTTCCTTGGTGCAGTGGCTGCGCGACAACCTACAGCTAATCCCCAACGCACCAGCGATTGAAAACCTCGCCCGAGA
AGTCGAAGACAACGGCGGCGTTCATGTTGTCCCAGCATTCACCGGACTGTTCGCACCACGTTGGCGCCCCGATGCTCGTG
GCGTCATTACAGGCCTCACCCGTTTTGCCAACCGCAAACACATCGCCCGCGCAGTCCTTGAAGCCAACGCCTTCCAAACC
CGCGAAGTTGTGGACGCCATGGCCAAAGACGCAGGCAAAGCCCTCGAATCCCTCCGCGTCGACGGTGCGATGGTGGAAAA
TGACCTCCTCATGCAAATGCAAGCCGACTTCCTCGGCATCGACGTCCAACGTCTCGAGGACGTAGAAACCACCGCCGTCG
GCGTCGCATTCGCTGCAGGTCTCGGCTCTGGATTCTTCAAAACAACTGACGAGATCGAAAAACTTATCGCAGTGAAGAAA
GTCTGGAACCCTGACATGAGCGAAGAAGAGCGCGAACGTCGCTATGCCGAATGGAATAGGGCAGTGGAGCATTCTTATGA
CCAGGCCTAG

Upstream 100 bases:

>100_bases
ACCAACGACGACGCCGGTGTAGCAGATGTATTGGAGTGGTGGTTCTAGAAGGTAGTTGTAAAACACTGCTTGGCGGATCA
ATACGTGCAAAAATAGGGCC

Downstream 100 bases:

>100_bases
CTGATTTGGGTCGGCCTTTAATTGGCTTTGAATTGGTAAAAACCCCGCCTCCTCAGGGAAGGTAGCGGGGTTTTCGGCTC
TAATTGAGACTAGTTCAGCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRISKANAYVAAIDQGTTSTRCIFIDAQGKVVSSASKEHRQIFPQQGWVEHDPEEIWDNIRSVVSQAMVSIDITPHEVAS
LGVTNQRETTVVWDKHTGEPVYNAIVWQDTRTSDICLEIAGEEGQEKWLDRTGLLINSYPSGPKIKWILDNVEGARERAE
KGDLLFGTMDTWVLWNLTGGVRGDDGDDAIHVTDVTNASRTLLMDLRTQQWDPELCEALDIPMSMLPEIRPSVGEFRSVR
HRGTLADVPITGVLGDQQAALFGQGGFHEGAAKNTYGTGLFLLMNTGTSLKISEHGLLSTIAYQREGSAPVYALEGSVSM
GGSLVQWLRDNLQLIPNAPAIENLAREVEDNGGVHVVPAFTGLFAPRWRPDARGVITGLTRFANRKHIARAVLEANAFQT
REVVDAMAKDAGKALESLRVDGAMVENDLLMQMQADFLGIDVQRLEDVETTAVGVAFAAGLGSGFFKTTDEIEKLIAVKK
VWNPDMSEEERERRYAEWNRAVEHSYDQA

Sequences:

>Translated_509_residues
MRISKANAYVAAIDQGTTSTRCIFIDAQGKVVSSASKEHRQIFPQQGWVEHDPEEIWDNIRSVVSQAMVSIDITPHEVAS
LGVTNQRETTVVWDKHTGEPVYNAIVWQDTRTSDICLEIAGEEGQEKWLDRTGLLINSYPSGPKIKWILDNVEGARERAE
KGDLLFGTMDTWVLWNLTGGVRGDDGDDAIHVTDVTNASRTLLMDLRTQQWDPELCEALDIPMSMLPEIRPSVGEFRSVR
HRGTLADVPITGVLGDQQAALFGQGGFHEGAAKNTYGTGLFLLMNTGTSLKISEHGLLSTIAYQREGSAPVYALEGSVSM
GGSLVQWLRDNLQLIPNAPAIENLAREVEDNGGVHVVPAFTGLFAPRWRPDARGVITGLTRFANRKHIARAVLEANAFQT
REVVDAMAKDAGKALESLRVDGAMVENDLLMQMQADFLGIDVQRLEDVETTAVGVAFAAGLGSGFFKTTDEIEKLIAVKK
VWNPDMSEEERERRYAEWNRAVEHSYDQA
>Mature_509_residues
MRISKANAYVAAIDQGTTSTRCIFIDAQGKVVSSASKEHRQIFPQQGWVEHDPEEIWDNIRSVVSQAMVSIDITPHEVAS
LGVTNQRETTVVWDKHTGEPVYNAIVWQDTRTSDICLEIAGEEGQEKWLDRTGLLINSYPSGPKIKWILDNVEGARERAE
KGDLLFGTMDTWVLWNLTGGVRGDDGDDAIHVTDVTNASRTLLMDLRTQQWDPELCEALDIPMSMLPEIRPSVGEFRSVR
HRGTLADVPITGVLGDQQAALFGQGGFHEGAAKNTYGTGLFLLMNTGTSLKISEHGLLSTIAYQREGSAPVYALEGSVSM
GGSLVQWLRDNLQLIPNAPAIENLAREVEDNGGVHVVPAFTGLFAPRWRPDARGVITGLTRFANRKHIARAVLEANAFQT
REVVDAMAKDAGKALESLRVDGAMVENDLLMQMQADFLGIDVQRLEDVETTAVGVAFAAGLGSGFFKTTDEIEKLIAVKK
VWNPDMSEEERERRYAEWNRAVEHSYDQA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=47.6377952755905, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4504007, Length=508, Percent_Identity=47.6377952755905, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=47.1734892787524, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=46.6403162055336, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI88196792, Length=515, Percent_Identity=30.0970873786408, Blast_Score=279, Evalue=6e-75,
Organism=Homo sapiens, GI164663828, Length=187, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1790361, Length=499, Percent_Identity=49.8997995991984, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1789987, Length=509, Percent_Identity=23.7721021611002, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789138, Length=518, Percent_Identity=19.3050193050193, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=43.2806324110672, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=527, Percent_Identity=24.0986717267552, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71986147, Length=259, Percent_Identity=28.1853281853282, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=556, Percent_Identity=35.9712230215827, Blast_Score=317, Evalue=3e-87,
Organism=Drosophila melanogaster, GI17864214, Length=515, Percent_Identity=43.1067961165049, Blast_Score=404, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655555, Length=506, Percent_Identity=40.1185770750988, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655560, Length=506, Percent_Identity=40.1185770750988, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655570, Length=506, Percent_Identity=40.1185770750988, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655574, Length=506, Percent_Identity=40.1185770750988, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24655565, Length=506, Percent_Identity=40.1185770750988, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24654573, Length=455, Percent_Identity=43.956043956044, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=34.2205323193916, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=34.1463414634146, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24656367, Length=521, Percent_Identity=28.9827255278311, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21357867, Length=532, Percent_Identity=28.0075187969925, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24656372, Length=355, Percent_Identity=29.8591549295775, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI45552909, Length=287, Percent_Identity=28.9198606271777, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_CORGB (A4QHT1)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139699.1
- ProteinModelPortal:   A4QHT1
- SMR:   A4QHT1
- STRING:   A4QHT1
- GeneID:   4993458
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2778
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   ATYFSGT
- ProtClustDB:   PRK00047
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 56146; Mature: 56146

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 17-17 BINDING 21-21 BINDING 87-87 BINDING 139-139 BINDING 256-256 BINDING 278-278 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISKANAYVAAIDQGTTSTRCIFIDAQGKVVSSASKEHRQIFPQQGWVEHDPEEIWDNI
CCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RSVVSQAMVSIDITPHEVASLGVTNQRETTVVWDKHTGEPVYNAIVWQDTRTSDICLEIA
HHHHHHHHHEECCCHHHHHHCCCCCCCCEEEEEECCCCCHHHEEEEECCCCCCCEEEEEC
GEEGQEKWLDRTGLLINSYPSGPKIKWILDNVEGARERAEKGDLLFGTMDTWVLWNLTGG
CCCCHHHHHHHCCCEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEECCEEEEEEECCC
VRGDDGDDAIHVTDVTNASRTLLMDLRTQQWDPELCEALDIPMSMLPEIRPSVGEFRSVR
CCCCCCCCEEEEEECCCCCCEEEEEHHCCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHH
HRGTLADVPITGVLGDQQAALFGQGGFHEGAAKNTYGTGLFLLMNTGTSLKISEHGLLST
HCCCEECCCEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHH
IAYQREGSAPVYALEGSVSMGGSLVQWLRDNLQLIPNAPAIENLAREVEDNGGVHVVPAF
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCEEEEECH
TGLFAPRWRPDARGVITGLTRFANRKHIARAVLEANAFQTREVVDAMAKDAGKALESLRV
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
DGAMVENDLLMQMQADFLGIDVQRLEDVETTAVGVAFAAGLGSGFFKTTDEIEKLIAVKK
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VWNPDMSEEERERRYAEWNRAVEHSYDQA
HCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRISKANAYVAAIDQGTTSTRCIFIDAQGKVVSSASKEHRQIFPQQGWVEHDPEEIWDNI
CCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RSVVSQAMVSIDITPHEVASLGVTNQRETTVVWDKHTGEPVYNAIVWQDTRTSDICLEIA
HHHHHHHHHEECCCHHHHHHCCCCCCCCEEEEEECCCCCHHHEEEEECCCCCCCEEEEEC
GEEGQEKWLDRTGLLINSYPSGPKIKWILDNVEGARERAEKGDLLFGTMDTWVLWNLTGG
CCCCHHHHHHHCCCEEECCCCCCEEEEEECCCHHHHHHHHCCCEEEEECCEEEEEEECCC
VRGDDGDDAIHVTDVTNASRTLLMDLRTQQWDPELCEALDIPMSMLPEIRPSVGEFRSVR
CCCCCCCCEEEEEECCCCCCEEEEEHHCCCCCHHHHHHHCCCHHHHHHCCCCHHHHHHHH
HRGTLADVPITGVLGDQQAALFGQGGFHEGAAKNTYGTGLFLLMNTGTSLKISEHGLLST
HCCCEECCCEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCHHHH
IAYQREGSAPVYALEGSVSMGGSLVQWLRDNLQLIPNAPAIENLAREVEDNGGVHVVPAF
HHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCEEEEECH
TGLFAPRWRPDARGVITGLTRFANRKHIARAVLEANAFQTREVVDAMAKDAGKALESLRV
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
DGAMVENDLLMQMQADFLGIDVQRLEDVETTAVGVAFAAGLGSGFFKTTDEIEKLIAVKK
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VWNPDMSEEERERRYAEWNRAVEHSYDQA
HCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA