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The map label for this gene is cysS

Identifier: 145296638

GI number: 145296638

Start: 2810836

End: 2812218

Strand: Reverse

Name: cysS

Synonym: cgR_2546

Alternate gene names: 145296638

Gene position: 2812218-2810836 (Counterclockwise)

Preceding gene: 145296639

Following gene: 145296637

Centisome position: 84.85

GC content: 52.13

Gene sequence:

>1383_bases
GTGACTCTACGCATCTTTGACACCGGTACCCGTACGCTTCGAGATTTTAAACCTGTTCAACCAGGTCATGCCTCGGTGTA
CCTGTGTGGTGCCACCCCGCAATCTTCACCCCACATTGGACATGTTCGTTCAGCAGTAGCGTTTGATATTTTGCGCCGCT
GGCTCATGGCTAAGGGTCTTGACGTGGCATTTGTTCGCAATGTCACTGATATCGATGACAAGATTCTCACCAAGGCATCT
GAAAATGGTCGCCCTTGGTGGGAATGGGTGTCTACTTATGAACGTGAATTCACCTGGACGTACAACACGTTGGGTGTGCT
TCCTCCATCAACGGAGCCTCGTGCAACAGGCCACGTCACTCAGATGATTGAGTACATGCAGCGCTTGATTGATAACGGCT
TTGCTTACGCCGTTGATGGCTCTGTGTACTTTGATGTCGCAGCGTGGTCCAAGGCTGAAGGATCTGACTATGGTTCTTTG
TCCGGAAACCGTGTGGAAGACATGGAGCAGGGCGAGCCTGATAACTTTGGCAAGCGGGGGCCACAGGACTTTGCTCTGTG
GAAGGCTGCCAAACCAGGTGAGCCGTCATGGCCAACCCCTTGGGGAGACGGCCGGCCGGGTTGGCATTTGGAATGCTCTG
CCATGGCAACCTACTATTTGGGTGAGCAATTTGATATTCACTGTGGTGGTTTGGATCTGCAATTTCCACACCATGAAAAT
GAAATTGCCCAGGCACATGCAGCTGGCGATAAATTTGCCAACTACTGGATGCACAATCACTGGGTAACAATGGCCGGCGA
GAAAATGTCCAAGTCTTTGGGCAATGTTTTGGCTGTGCCGGAAATGCTGAAGCAGGTTCGTCCTGTCGAGCTTCGTTATT
ACCTTGGGTCTGCCCATTACCGTTCTGTCCTTGAATATTCCGAGAGCGCTTTGAGTGAAGCTGCGGTGGGTTACCGTCGC
ATTGAGTCTTTCCTTGAGCGTGTGGGGGATGTTGAGGTAGGCGAGTGGACGCCAGGTTTTGAAGCTGCGATGGATGAGGA
TATTGCAGTTCCTAAGGCTTTGGCTGAAATCCATAACGCTGTCCGCGAAGGTAACGCTGCTTTGGATAAGGGCGATCGCG
AGGCGGCGAAGAAGCTTGCTTCTTCAGTTCGTGCGATGACTGGTGTTTTGGGCTTCGACCCCGTTGAATGGGGTTCAGAT
GCAGGCGCTGATGGCAAGGCAGATAAGGCGCTTGATGTGCTGATTTCTTCGGAGCTTGAGCGTCGTGCAACTGCTCGTGC
TGAGAAGAATTGGACGGTTGCTGATGAGGTTCGAGATCGTCTTGCCGATGCCGGTATTGAGGTTGTGGATACCGCAGATG
GCGCTACATGGAAATTGCAGTAA

Upstream 100 bases:

>100_bases
AGCAGGAGCCAACCTGCTCAACGTCGCAAAGAAAGAAGCGGTGCCAGCAACACCATAAGTTGAAACCTTGAGTGTTCGCA
CACAGGTTAGACTAGGGGAC

Downstream 100 bases:

>100_bases
TTACAGACACTTTTAAGGAGACAATTTAAACATGGCAGGAAATGACAGTCGTCGAGGCGGCTTACGCAAGACCAATAAAA
AAGGTGCAACCAAGGGCAGT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTLRIFDTGTRTLRDFKPVQPGHASVYLCGATPQSSPHIGHVRSAVAFDILRRWLMAKGLDVAFVRNVTDIDDKILTKAS
ENGRPWWEWVSTYEREFTWTYNTLGVLPPSTEPRATGHVTQMIEYMQRLIDNGFAYAVDGSVYFDVAAWSKAEGSDYGSL
SGNRVEDMEQGEPDNFGKRGPQDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATYYLGEQFDIHCGGLDLQFPHHEN
EIAQAHAAGDKFANYWMHNHWVTMAGEKMSKSLGNVLAVPEMLKQVRPVELRYYLGSAHYRSVLEYSESALSEAAVGYRR
IESFLERVGDVEVGEWTPGFEAAMDEDIAVPKALAEIHNAVREGNAALDKGDREAAKKLASSVRAMTGVLGFDPVEWGSD
AGADGKADKALDVLISSELERRATARAEKNWTVADEVRDRLADAGIEVVDTADGATWKLQ

Sequences:

>Translated_460_residues
MTLRIFDTGTRTLRDFKPVQPGHASVYLCGATPQSSPHIGHVRSAVAFDILRRWLMAKGLDVAFVRNVTDIDDKILTKAS
ENGRPWWEWVSTYEREFTWTYNTLGVLPPSTEPRATGHVTQMIEYMQRLIDNGFAYAVDGSVYFDVAAWSKAEGSDYGSL
SGNRVEDMEQGEPDNFGKRGPQDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATYYLGEQFDIHCGGLDLQFPHHEN
EIAQAHAAGDKFANYWMHNHWVTMAGEKMSKSLGNVLAVPEMLKQVRPVELRYYLGSAHYRSVLEYSESALSEAAVGYRR
IESFLERVGDVEVGEWTPGFEAAMDEDIAVPKALAEIHNAVREGNAALDKGDREAAKKLASSVRAMTGVLGFDPVEWGSD
AGADGKADKALDVLISSELERRATARAEKNWTVADEVRDRLADAGIEVVDTADGATWKLQ
>Mature_459_residues
TLRIFDTGTRTLRDFKPVQPGHASVYLCGATPQSSPHIGHVRSAVAFDILRRWLMAKGLDVAFVRNVTDIDDKILTKASE
NGRPWWEWVSTYEREFTWTYNTLGVLPPSTEPRATGHVTQMIEYMQRLIDNGFAYAVDGSVYFDVAAWSKAEGSDYGSLS
GNRVEDMEQGEPDNFGKRGPQDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATYYLGEQFDIHCGGLDLQFPHHENE
IAQAHAAGDKFANYWMHNHWVTMAGEKMSKSLGNVLAVPEMLKQVRPVELRYYLGSAHYRSVLEYSESALSEAAVGYRRI
ESFLERVGDVEVGEWTPGFEAAMDEDIAVPKALAEIHNAVREGNAALDKGDREAAKKLASSVRAMTGVLGFDPVEWGSDA
GADGKADKALDVLISSELERRATARAEKNWTVADEVRDRLADAGIEVVDTADGATWKLQ

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=374, Percent_Identity=38.7700534759358, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI10835051, Length=299, Percent_Identity=39.7993311036789, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI21269877, Length=299, Percent_Identity=39.7993311036789, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI62240992, Length=299, Percent_Identity=39.7993311036789, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI303304987, Length=299, Percent_Identity=39.7993311036789, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1786737, Length=464, Percent_Identity=42.4568965517241, Blast_Score=336, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17509797, Length=296, Percent_Identity=37.1621621621622, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17509795, Length=296, Percent_Identity=37.1621621621622, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324082, Length=390, Percent_Identity=33.0769230769231, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI19922218, Length=502, Percent_Identity=31.4741035856574, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI19922364, Length=269, Percent_Identity=40.5204460966543, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CORGB (A4QH41)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139459.1
- ProteinModelPortal:   A4QH41
- SMR:   A4QH41
- STRING:   A4QH41
- GeneID:   4991864
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2546
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 51049; Mature: 50918

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRIFDTGTRTLRDFKPVQPGHASVYLCGATPQSSPHIGHVRSAVAFDILRRWLMAKGL
CEEEEEECCCHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKASENGRPWWEWVSTYEREFTWTYNTLGVLPPSTEPRATGHVT
CEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECEEECCCCCCCCCCCHHHH
QMIEYMQRLIDNGFAYAVDGSVYFDVAAWSKAEGSDYGSLSGNRVEDMEQGEPDNFGKRG
HHHHHHHHHHHCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
PQDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATYYLGEQFDIHCGGLDLQFPHHEN
CCHHHHEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEEECCEEEECCCCCH
EIAQAHAAGDKFANYWMHNHWVTMAGEKMSKSLGNVLAVPEMLKQVRPVELRYYLGSAHY
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
RSVLEYSESALSEAAVGYRRIESFLERVGDVEVGEWTPGFEAAMDEDIAVPKALAEIHNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
VREGNAALDKGDREAAKKLASSVRAMTGVLGFDPVEWGSDAGADGKADKALDVLISSELE
HHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRATARAEKNWTVADEVRDRLADAGIEVVDTADGATWKLQ
HHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEC
>Mature Secondary Structure 
TLRIFDTGTRTLRDFKPVQPGHASVYLCGATPQSSPHIGHVRSAVAFDILRRWLMAKGL
EEEEEECCCHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
DVAFVRNVTDIDDKILTKASENGRPWWEWVSTYEREFTWTYNTLGVLPPSTEPRATGHVT
CEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECEEECCCCCCCCCCCHHHH
QMIEYMQRLIDNGFAYAVDGSVYFDVAAWSKAEGSDYGSLSGNRVEDMEQGEPDNFGKRG
HHHHHHHHHHHCCCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
PQDFALWKAAKPGEPSWPTPWGDGRPGWHLECSAMATYYLGEQFDIHCGGLDLQFPHHEN
CCHHHHEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEEECCEEEECCCCCH
EIAQAHAAGDKFANYWMHNHWVTMAGEKMSKSLGNVLAVPEMLKQVRPVELRYYLGSAHY
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHH
RSVLEYSESALSEAAVGYRRIESFLERVGDVEVGEWTPGFEAAMDEDIAVPKALAEIHNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHH
VREGNAALDKGDREAAKKLASSVRAMTGVLGFDPVEWGSDAGADGKADKALDVLISSELE
HHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRATARAEKNWTVADEVRDRLADAGIEVVDTADGATWKLQ
HHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA