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The map label for this gene is bioD

Identifier: 145296601

GI number: 145296601

Start: 2771036

End: 2771710

Strand: Direct

Name: bioD

Synonym: cgR_2509

Alternate gene names: 145296601

Gene position: 2771036-2771710 (Clockwise)

Preceding gene: 145296600

Following gene: 145296607

Centisome position: 83.61

GC content: 52.74

Gene sequence:

>675_bases
ATGCCATTTTTATTTGTCAGCGGCACCGGAACCGGGGTTGGAAAGACCTTCTCCACAGCCGTTTTGGTTCGTTACTTAGC
CGATCAAGGACACGATGTTCTGCCCGTAAAGCTCGTCCAAACAGGTGAACTTCCAGGCGAAGGAGACATCTTCACCATTG
AACGCTTGACTGGAATTGCTGGAGAGGAATTTGCTCGTTTCAAAGACCCTCTTGCGCCAAATCTGGCAGCCCGACGAGAG
GGGATCGAGCCAATACAGTTTGATCAGATTATCTCGTGGCTTCGTGGTTTTGACGACCCAGATCGCATCATTGTGGTGGA
GGGCGCTGGTGGCCTGCTGGTCAGATTAGGGGAAGATTTCACCCTGGCAGATGTTGCCTCCGCTTTGAATGCACCCTTAG
TGATTGTGACAAGCACCGGATTGGGAAGCCTCAACGCTGCTGAATTAAGCGTTGAGGCAGCAAACCGCCGAGGACTCACA
GTGTTGGGAGTCCTCGGCGGTTCGATCCCTCAAAATCCTGATCTAGCTACGATGCTTAATCTCGAAGAATTTGAGAGAGT
CACCGGCGTGCCCTTTTGGGGAGCTTTGCCGGAAGGGTTGTCACGGGTGGAGGGGTTCGTCGAAAAGCAATCTTTTCCGG
CCCTTGATGCCTTTAAGAAACCGCCGGCAAGGTGA

Upstream 100 bases:

>100_bases
TGGACGCTTGCTCTATGTCATGCCCCCATATATCACCACGTCAGAGCAATGCGCACAGATCTGCGCCGCGCTTCATGCTG
CAGTTAAAGGAAAATAAACC

Downstream 100 bases:

>100_bases
TCGTGAACACCGTGCCTTCGCCTTGCACGCTGTCGACGGTGACTGTGCCGCCGTGGCCTTCGACCAGGGATTTCGTGATC
GCAAGGCCGAGGCCCGATCC

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPFLFVSGTGTGVGKTFSTAVLVRYLADQGHDVLPVKLVQTGELPGEGDIFTIERLTGIAGEEFARFKDPLAPNLAARRE
GIEPIQFDQIISWLRGFDDPDRIIVVEGAGGLLVRLGEDFTLADVASALNAPLVIVTSTGLGSLNAAELSVEAANRRGLT
VLGVLGGSIPQNPDLATMLNLEEFERVTGVPFWGALPEGLSRVEGFVEKQSFPALDAFKKPPAR

Sequences:

>Translated_224_residues
MPFLFVSGTGTGVGKTFSTAVLVRYLADQGHDVLPVKLVQTGELPGEGDIFTIERLTGIAGEEFARFKDPLAPNLAARRE
GIEPIQFDQIISWLRGFDDPDRIIVVEGAGGLLVRLGEDFTLADVASALNAPLVIVTSTGLGSLNAAELSVEAANRRGLT
VLGVLGGSIPQNPDLATMLNLEEFERVTGVPFWGALPEGLSRVEGFVEKQSFPALDAFKKPPAR
>Mature_223_residues
PFLFVSGTGTGVGKTFSTAVLVRYLADQGHDVLPVKLVQTGELPGEGDIFTIERLTGIAGEEFARFKDPLAPNLAARREG
IEPIQFDQIISWLRGFDDPDRIIVVEGAGGLLVRLGEDFTLADVASALNAPLVIVTSTGLGSLNAAELSVEAANRRGLTV
LGVLGGSIPQNPDLATMLNLEEFERVTGVPFWGALPEGLSRVEGFVEKQSFPALDAFKKPPAR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=174, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_CORGB (A4QH04)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139422.1
- ProteinModelPortal:   A4QH04
- SMR:   A4QH04
- STRING:   A4QH04
- GeneID:   4993286
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2509
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   VVIGSWP
- ProtClustDB:   PRK00090
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23857; Mature: 23725

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLFVSGTGTGVGKTFSTAVLVRYLADQGHDVLPVKLVQTGELPGEGDIFTIERLTGIA
CCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEHHHHHCCC
GEEFARFKDPLAPNLAARREGIEPIQFDQIISWLRGFDDPDRIIVVEGAGGLLVRLGEDF
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCC
TLADVASALNAPLVIVTSTGLGSLNAAELSVEAANRRGLTVLGVLGGSIPQNPDLATMLN
CHHHHHHHHCCCEEEEEECCCCCCCHHEEEEECCCCCCCEEEEEECCCCCCCCCHHEEEC
LEEFERVTGVPFWGALPEGLSRVEGFVEKQSFPALDAFKKPPAR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PFLFVSGTGTGVGKTFSTAVLVRYLADQGHDVLPVKLVQTGELPGEGDIFTIERLTGIA
CEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEHHHHHCCC
GEEFARFKDPLAPNLAARREGIEPIQFDQIISWLRGFDDPDRIIVVEGAGGLLVRLGEDF
HHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCC
TLADVASALNAPLVIVTSTGLGSLNAAELSVEAANRRGLTVLGVLGGSIPQNPDLATMLN
CHHHHHHHHCCCEEEEEECCCCCCCHHEEEEECCCCCCCEEEEEECCCCCCCCCHHEEEC
LEEFERVTGVPFWGALPEGLSRVEGFVEKQSFPALDAFKKPPAR
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA