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The map label for this gene is priA [H]

Identifier: 145295723

GI number: 145295723

Start: 1835241

End: 1837307

Strand: Reverse

Name: priA [H]

Synonym: cgR_1650

Alternate gene names: 145295723

Gene position: 1837307-1835241 (Counterclockwise)

Preceding gene: 145295724

Following gene: 145295722

Centisome position: 55.44

GC content: 57.18

Gene sequence:

>2067_bases
ATGGCAAAAACCCGCGTCCCCGCTCCTGAAAAGTCGGTGGCGCGGGTTTTACCTCTTTTGGGGTTACCTCACCTGGATCG
ACTGTTTGATTACCGCATCAGCGAAGACCAACACGATGATGTGCAACCTGGCGTGCGAGTGCGCGTGCGTTTTGGTGGAC
GTTTAGTTGATGCCATTGTGATGTCACGCACCGCGCAAACCTCGCACGAGGGAAAGCTGATGTGGCTGGATCGGGTGATT
TCGCCGATCGTGGTGTATCCACCTCAAACAGCAAAGCTAATTGAGCAACTCAGTGATCGCTATGGCGGGGTACGTTCGGA
TCTCATCCGTTCGGCGCTACCGGCGCGGCATGCTGGGGCGGAAGAGGCAGATACCTCCACGTCATGGGAGTCATTGGGCG
AGGTTAAAGAACCCGATTTATCGTCGTGGTCTGCGTATCAGCATGGTCAATCATTTGTTGACGCCGTCTTGGCGGGAACA
ACTGCGCGGGCGTCATGGCAGATTGCTCCCGGAGATAATTGGGCGCTGGCTTTGGCTTCTTTGGCGGTCAAGGTTGTCAA
AGACGGCGGCGGAGCGCTTCTCGTAGTGCCTGATCAGCGCGATCTCGACCGTTTGGAAGCTGCGCTTCGAGGTTTGGTTG
CGGCGAAACAAATCACTGTGCTTAATTCAGGTCTTGGCCCGCAGGCACGATATCGGCGTTTCCTATCGGTTCTCAGTGGG
CAGGGACGACTGATTATTGGAACCAGAAGTGCCGCTTTTGCACCCGTGAAGGATCTGAAACTGGCCGTCATTTTAAATGA
TGGCGACGACAACCTCGTTGATCCTAGAGCGCCCTATGCCCACGCCAGGGAAGTGCTGACCACGCGTTCCAGTTTGGAAG
CAAGCTCCTTGATTATTGCGGGACATGCGCGGACCGCGGAAACCCAATTGCTGGTGGAGTCGGGATGGATGCACAATCTC
ATCGCACCGAGGGATACCATTCGCACTAGGATGCCGCGTATTCAGGCAGTGGGCGATTCCGATTTCCAGATGGAACGCGA
TCCAATGGCCCGATCAGCGCGGCTGCCTGGCATTGCGTTTCATGCGGTGCGCAGCGCCTTAGAACGTGATCAACCAGCAC
TTATCCAGGTACCAAGGAAAGGCTACGTGCCAACCTTGGCGTGTGGAAACTGCCGCGCCCCAGCGCGGTGCCGGCACTGT
AATGGACCTGTGGGACTTCCCCAGGGAAGCTCTGATCTAGCGGGAGTGCCCACTTGCCGATGGTGCGGACGCCCTGATTC
GCGGTTTAAGTGCCAAAACTGCGGCTCTCCAAAACTGCGTGCTGTGGTGCTGGGAACGGAACGCACAGCAGAAGAACTGG
GCCGCGCGTTCCCGTCTGTGCGGGTAATTACCTCTGGCGGCAACAAGGTGGTGGATTCGGTGGAAAACCGAGCCAGCATT
GTGGTGTCCACGCCAGGTGCAGAACCTTATGTGGCCAACTCGCCGGAGAGACCAGAGAAATCGGAGAAACCAGAGCACAA
GGGCGCTTACGGTGCCTTGTTATTGCTGGATACCTGGGCGTTGATGGGTCGGCAAGATCTGCGCGCCATGGAGGACGCGC
TGCACAAGTGGGCAGCGGCGGCCACGCTGGTGCATTCTCATCTGCACCAGGGTCAAGTCATCGTGGTTGCAGATCCATCG
TTTCCTGCTGTGCAATCGTTGATTCGGTGGGATATGGCAGGTGCTGCAGCGCAAGAGTTGGCTAGCCGACGCGAGGTTAT
GTTCCCGCCTTCCGTACACATGGCAGCAATCGATGGGGCTACCGCTGCATTGGAAAGTTTCTTGGATTTGGCGGAGCTTC
CCGATCATGCTGAAGTCCTCGGCCCTGTTGATCTGCCACCGGGTGTGAGTTTGCCTGGTGAATATGATGAGCAGCGCTTT
GGTCCGCCGCAGCGCCTTCTCATCAGAACTCCATTGGGACCGCGGTCTGAGTTGGGTAGAGCGCTGCGCTCAGCCCAGGT
GGCGCGTGCGGTGAGGAAGAATGATTTGCCGTTGCGTATTCAGATGGATCCGATTCACATCGGATAA

Upstream 100 bases:

>100_bases
GCACTGATTTGGACCTTCCTTGGGAGGCTATCGACCGCGTTGATGAACTTCGCGCAGCCCTCAAGTTGGCCTAAAAATCT
GATGTAGTATCTTCGGATTC

Downstream 100 bases:

>100_bases
AACTGCTGGTGAAAGGCCTAATGGGGTCGTGTCCCACGTGGGATATAAGATGGGGAGACAAGATCATCCACCCGTAGGAA
TTATTGTTGAAGGAGCCTTC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVI
SPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGT
TARASWQIAPGDNWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNL
IAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRAPARCRHC
NGPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
VVSTPGAEPYVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPS
FPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRF
GPPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG

Sequences:

>Translated_688_residues
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVI
SPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGT
TARASWQIAPGDNWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNL
IAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRAPARCRHC
NGPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
VVSTPGAEPYVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPS
FPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRF
GPPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG
>Mature_687_residues
AKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIVMSRTAQTSHEGKLMWLDRVIS
PIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGAEEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTT
ARASWQIAPGDNWALALASLAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSGQ
GRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIAGHARTAETQLLVESGWMHNLI
APRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAFHAVRSALERDQPALIQVPRKGYVPTLACGNCRAPARCRHCN
GPVGLPQGSSDLAGVPTCRWCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASIV
VSTPGAEPYVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAAATLVHSHLHQGQVIVVADPSF
PAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGATAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFG
PPQRLLIRTPLGPRSELGRALRSAQVARAVRKNDLPLRIQMDPIHIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=445, Percent_Identity=26.7415730337079, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 74578; Mature: 74447

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIV
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHH
MSRTAQTSHEGKLMWLDRVISPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGA
HHHHHCCCCCCCEEEHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCC
EEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTTARASWQIAPGDNWALALAS
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
LAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
HHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCC
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GHARTAETQLLVESGWMHNLIAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAF
CCCCCCHHHEEECCCCHHCCCCCHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCHHH
HAVRSALERDQPALIQVPRKGYVPTLACGNCRAPARCRHCNGPVGLPQGSSDLAGVPTCR
HHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHH
WCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
CCCCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEE
VVSTPGAEPYVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ATLVHSHLHQGQVIVVADPSFPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGA
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHH
TAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFGPPQRLLIRTPLGPRSELGR
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHEEEECCCCCHHHHHH
ALRSAQVARAVRKNDLPLRIQMDPIHIG
HHHHHHHHHHHHHCCCCEEEEECCEECC
>Mature Secondary Structure 
AKTRVPAPEKSVARVLPLLGLPHLDRLFDYRISEDQHDDVQPGVRVRVRFGGRLVDAIV
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHH
MSRTAQTSHEGKLMWLDRVISPIVVYPPQTAKLIEQLSDRYGGVRSDLIRSALPARHAGA
HHHHHCCCCCCCEEEHHHHHCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHCCC
EEADTSTSWESLGEVKEPDLSSWSAYQHGQSFVDAVLAGTTARASWQIAPGDNWALALAS
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
LAVKVVKDGGGALLVVPDQRDLDRLEAALRGLVAAKQITVLNSGLGPQARYRRFLSVLSG
HHHHHEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCC
QGRLIIGTRSAAFAPVKDLKLAVILNDGDDNLVDPRAPYAHAREVLTTRSSLEASSLIIA
CCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEE
GHARTAETQLLVESGWMHNLIAPRDTIRTRMPRIQAVGDSDFQMERDPMARSARLPGIAF
CCCCCCHHHEEECCCCHHCCCCCHHHHHHHCCCEEECCCCCCCCCCCHHHHHCCCCCHHH
HAVRSALERDQPALIQVPRKGYVPTLACGNCRAPARCRHCNGPVGLPQGSSDLAGVPTCR
HHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHH
WCGRPDSRFKCQNCGSPKLRAVVLGTERTAEELGRAFPSVRVITSGGNKVVDSVENRASI
CCCCCCCCCEECCCCCCCEEEEEECCHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCEE
VVSTPGAEPYVANSPERPEKSEKPEHKGAYGALLLLDTWALMGRQDLRAMEDALHKWAAA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ATLVHSHLHQGQVIVVADPSFPAVQSLIRWDMAGAAAQELASRREVMFPPSVHMAAIDGA
HHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCHH
TAALESFLDLAELPDHAEVLGPVDLPPGVSLPGEYDEQRFGPPQRLLIRTPLGPRSELGR
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHEEEECCCCCHHHHHH
ALRSAQVARAVRKNDLPLRIQMDPIHIG
HHHHHHHHHHHHHCCCCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]