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The map label for this gene is def

Identifier: 145295722

GI number: 145295722

Start: 1834623

End: 1835132

Strand: Reverse

Name: def

Synonym: cgR_1649

Alternate gene names: 145295722

Gene position: 1835132-1834623 (Counterclockwise)

Preceding gene: 145295723

Following gene: 145295721

Centisome position: 55.37

GC content: 54.12

Gene sequence:

>510_bases
ATGGCAGTTCGTGAAGTCAGATTATTTGGAGATCCCGTACTTGTGAGTCGGGCAGATGAGGTCGTGGACTTCGATGAATC
ACTGTCCACCCTGATCGATGACATGTTTGACACCATGGAAGATGCCGGTGGCGTTGGTCTAGCAGCAAACCAGGTGGGTG
TTTTAAGGCGCGTGTTCGTGTTTGATACCTCCCACCAGGAGGGCGGATTGCGCGGACATGTGATCAACCCGGTGTGGGAG
CCTCTCACTGAGGACACCCAGACTGGCAAAGAGGGCTGTTTGTCCATCCCTGATGTCTCGGCTGAGACCACCCGCTATGA
AACGGTTCGGCTGTCTGGCCAGGACCGCGACGGCAATCCAGTGGGGTTTGTGGCCAATGGATTGTTGGCCAGGTGCATCC
AACACGAAACTGATCACCTTGATGGCGTGCTGTTTTTGAAGCGCCTTGATCCAGCTGAACGTAAAGCAGCCATGGGCGTT
ATTCGCGCGTCTGCATGGTTTAACAAATAA

Upstream 100 bases:

>100_bases
GTGAAAGGCCTAATGGGGTCGTGTCCCACGTGGGATATAAGATGGGGAGACAAGATCATCCACCCGTAGGAATTATTGTT
GAAGGAGCCTTCCCACAACC

Downstream 100 bases:

>100_bases
TGAGTGGAAAGGGCACTTCATGCGCCTAGTTTTTGCCGGAACTCCGGAACCTGCAGTGGTGGCACTGCAAAAGCTCATTG
ATTCTGATCATGAGGTCGTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MAVREVRLFGDPVLVSRADEVVDFDESLSTLIDDMFDTMEDAGGVGLAANQVGVLRRVFVFDTSHQEGGLRGHVINPVWE
PLTEDTQTGKEGCLSIPDVSAETTRYETVRLSGQDRDGNPVGFVANGLLARCIQHETDHLDGVLFLKRLDPAERKAAMGV
IRASAWFNK

Sequences:

>Translated_169_residues
MAVREVRLFGDPVLVSRADEVVDFDESLSTLIDDMFDTMEDAGGVGLAANQVGVLRRVFVFDTSHQEGGLRGHVINPVWE
PLTEDTQTGKEGCLSIPDVSAETTRYETVRLSGQDRDGNPVGFVANGLLARCIQHETDHLDGVLFLKRLDPAERKAAMGV
IRASAWFNK
>Mature_168_residues
AVREVRLFGDPVLVSRADEVVDFDESLSTLIDDMFDTMEDAGGVGLAANQVGVLRRVFVFDTSHQEGGLRGHVINPVWEP
LTEDTQTGKEGCLSIPDVSAETTRYETVRLSGQDRDGNPVGFVANGLLARCIQHETDHLDGVLFLKRLDPAERKAAMGVI
RASAWFNK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=32.7044025157233, Blast_Score=70, Evalue=7e-13,
Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=42.3076923076923, Blast_Score=110, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24645728, Length=193, Percent_Identity=32.6424870466321, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=30.0613496932515, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_CORGL (Q8NQ46)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927152
- RefSeq:   NP_600815.1
- RefSeq:   YP_225885.1
- ProteinModelPortal:   Q8NQ46
- SMR:   Q8NQ46
- GeneID:   1019569
- GeneID:   3343555
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1804
- KEGG:   cgl:NCgl1539
- HOGENOM:   HBG665227
- OMA:   AIREAEW
- PhylomeDB:   Q8NQ46
- ProtClustDB:   PRK00150
- BioCyc:   CGLU196627:CG1804-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18543; Mature: 18412

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVREVRLFGDPVLVSRADEVVDFDESLSTLIDDMFDTMEDAGGVGLAANQVGVLRRVFV
CCCEEEEECCCCEEECCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FDTSHQEGGLRGHVINPVWEPLTEDTQTGKEGCLSIPDVSAETTRYETVRLSGQDRDGNP
EECCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCC
VGFVANGLLARCIQHETDHLDGVLFLKRLDPAERKAAMGVIRASAWFNK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVREVRLFGDPVLVSRADEVVDFDESLSTLIDDMFDTMEDAGGVGLAANQVGVLRRVFV
CCEEEEECCCCEEECCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FDTSHQEGGLRGHVINPVWEPLTEDTQTGKEGCLSIPDVSAETTRYETVRLSGQDRDGNP
EECCCCCCCCCCCCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCC
VGFVANGLLARCIQHETDHLDGVLFLKRLDPAERKAAMGVIRASAWFNK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626