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The map label for this gene is sseA [H]

Identifier: 145295558

GI number: 145295558

Start: 1646090

End: 1646959

Strand: Direct

Name: sseA [H]

Synonym: cgR_1485

Alternate gene names: 145295558

Gene position: 1646090-1646959 (Clockwise)

Preceding gene: 145295556

Following gene: 145295559

Centisome position: 49.67

GC content: 52.18

Gene sequence:

>870_bases
ATGACTTCTCTTAAAGTAACTTCGTCCACAGATGCAACCAATAACAATGATGCCCATTTTCCTGAAGGTCCAGTGGTAAC
TGTTGACTGGTTGTCACACAACCTTGACCGGGATGATGTCATCGTGTTGTGTGCCACAATGGAGGATGATGAAATTGCGC
GTCAAGCGGGAATTCCGGGGGCATTTCTCGCTGACTTGGAAGGAGATTTCTCAGATCCACATTCCGAGCTTCCACACACC
GCGCCACCGAATTTGGTGGGTTTGCTAGAAAGCTACGGCATTAGCACCGATTCCACGGTGGTTGTTTATGATCTGCACGG
CCTCATGGTTGCACCGCGGGTGTGGTGGCTTCTTCGTATTGCTGGATTAAGCAGTATTGGTGTGCTTGATGGCGGATTGC
CAGCCTGGGTTGATGCTGGTCTTCCGACGGAACCGTTGTCGCTACCTACAGGTGGTGGAAGGATCAGCGCAGAACCTCAG
CCGGATTTGCTCGTTGGTGCTTCTGGAGTGGAACGTGCGATTGCACGTTCAAGCAAGGCAGTGATTGATGCTCGCAATGC
GAGCCGATTCGCTGGCGTTGAAGAAGAGCCCCGTCCAGGCCTGCGAAAAGGGTCGATCCCTGGAAGCGTCAACATTCCCT
TCACTGATATTTCTGATGAGCATGGTTTTGTTCGGCCAGCAGAAGAGCTGAAGGAATTGATCTTCAGCCGCACAAATGGA
GCGCAGTCGTTGGTCTTTAGCTGTGGCTCTGGAGTCACAGCATGTGTTGATGCCTACGCTGCAGTTATCGCAGGTTATGA
CGACGTTGTAGTGTATGAGGGCTCCTGGGCGGAATGGGGCAACCCGGCAAACCAAAAGCCAATTGCTTAA

Upstream 100 bases:

>100_bases
GGTGGGAGAGTGCTACTATCTGCGGTTTTGTGAATTAAATGCTGTGAACTATCGCGAACTCCTCCCATTCAAGTGGTGTT
AAGCACTAAGATGGCAGGTT

Downstream 100 bases:

>100_bases
CGCCAGCTATGATAACCACTCATGAATGATTTCGATCCAGCACTGATTTCCGCGATGAGCCTTATCCCTCAGACGAGGTC
TCGGATGGAATCAATCTTGT

Product: hypothetical protein

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTSLKVTSSTDATNNNDAHFPEGPVVTVDWLSHNLDRDDVIVLCATMEDDEIARQAGIPGAFLADLEGDFSDPHSELPHT
APPNLVGLLESYGISTDSTVVVYDLHGLMVAPRVWWLLRIAGLSSIGVLDGGLPAWVDAGLPTEPLSLPTGGGRISAEPQ
PDLLVGASGVERAIARSSKAVIDARNASRFAGVEEEPRPGLRKGSIPGSVNIPFTDISDEHGFVRPAEELKELIFSRTNG
AQSLVFSCGSGVTACVDAYAAVIAGYDDVVVYEGSWAEWGNPANQKPIA

Sequences:

>Translated_289_residues
MTSLKVTSSTDATNNNDAHFPEGPVVTVDWLSHNLDRDDVIVLCATMEDDEIARQAGIPGAFLADLEGDFSDPHSELPHT
APPNLVGLLESYGISTDSTVVVYDLHGLMVAPRVWWLLRIAGLSSIGVLDGGLPAWVDAGLPTEPLSLPTGGGRISAEPQ
PDLLVGASGVERAIARSSKAVIDARNASRFAGVEEEPRPGLRKGSIPGSVNIPFTDISDEHGFVRPAEELKELIFSRTNG
AQSLVFSCGSGVTACVDAYAAVIAGYDDVVVYEGSWAEWGNPANQKPIA
>Mature_288_residues
TSLKVTSSTDATNNNDAHFPEGPVVTVDWLSHNLDRDDVIVLCATMEDDEIARQAGIPGAFLADLEGDFSDPHSELPHTA
PPNLVGLLESYGISTDSTVVVYDLHGLMVAPRVWWLLRIAGLSSIGVLDGGLPAWVDAGLPTEPLSLPTGGGRISAEPQP
DLLVGASGVERAIARSSKAVIDARNASRFAGVEEEPRPGLRKGSIPGSVNIPFTDISDEHGFVRPAEELKELIFSRTNGA
QSLVFSCGSGVTACVDAYAAVIAGYDDVVVYEGSWAEWGNPANQKPIA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI61835204, Length=227, Percent_Identity=36.1233480176211, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI194473681, Length=227, Percent_Identity=36.1233480176211, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI194473668, Length=227, Percent_Identity=36.1233480176211, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI17402865, Length=259, Percent_Identity=34.3629343629344, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI87082121, Length=270, Percent_Identity=35.5555555555556, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI87081967, Length=250, Percent_Identity=28.4, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561888, Length=289, Percent_Identity=23.8754325259516, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71997283, Length=294, Percent_Identity=23.1292517006803, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30513; Mature: 30382

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLKVTSSTDATNNNDAHFPEGPVVTVDWLSHNLDRDDVIVLCATMEDDEIARQAGIPG
CCCEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCEEEEEEECCHHHHHHHHCCCC
AFLADLEGDFSDPHSELPHTAPPNLVGLLESYGISTDSTVVVYDLHGLMVAPRVWWLLRI
EEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHH
AGLSSIGVLDGGLPAWVDAGLPTEPLSLPTGGGRISAEPQPDLLVGASGVERAIARSSKA
HCCCCCCEECCCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCEEEECHHHHHHHHHCCHH
VIDARNASRFAGVEEEPRPGLRKGSIPGSVNIPFTDISDEHGFVRPAEELKELIFSRTNG
EEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AQSLVFSCGSGVTACVDAYAAVIAGYDDVVVYEGSWAEWGNPANQKPIA
HHHHEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
TSLKVTSSTDATNNNDAHFPEGPVVTVDWLSHNLDRDDVIVLCATMEDDEIARQAGIPG
CCEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCEEEEEEECCHHHHHHHHCCCC
AFLADLEGDFSDPHSELPHTAPPNLVGLLESYGISTDSTVVVYDLHGLMVAPRVWWLLRI
EEEECCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHH
AGLSSIGVLDGGLPAWVDAGLPTEPLSLPTGGGRISAEPQPDLLVGASGVERAIARSSKA
HCCCCCCEECCCCHHHCCCCCCCCCCCCCCCCCEECCCCCCCEEEECHHHHHHHHHCCHH
VIDARNASRFAGVEEEPRPGLRKGSIPGSVNIPFTDISDEHGFVRPAEELKELIFSRTNG
EEECCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
AQSLVFSCGSGVTACVDAYAAVIAGYDDVVVYEGSWAEWGNPANQKPIA
HHHHEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]