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The map label for this gene is menE [H]

Identifier: 145294572

GI number: 145294572

Start: 582762

End: 583997

Strand: Reverse

Name: menE [H]

Synonym: cgR_0523

Alternate gene names: 145294572

Gene position: 583997-582762 (Counterclockwise)

Preceding gene: 145294573

Following gene: 145294570

Centisome position: 17.62

GC content: 52.35

Gene sequence:

>1236_bases
TTGGCGCAGGTGCATGGAGCATCGACGGGGTTCTGGCAAAACGCAAGGCCTAAACCTAGCGCCACAACTCCGAATTCTGA
ACCATCGGCACTAGAATCTCGAAATATGAATACTCGCGTCCTCGAAGCACTACCTGTTGATCTTGCAGATCCCACCGCAA
TTCTGGGAGATCTCGAGGACGCAATCTCTGGGAAGAAAACTTTCCTCCCCATCCCTGTACAAGATAAAACCCGTGCACAG
TTGCTACGCGATTCTCAACGAGTAGGCCTCGCCATCGATCCTTCGATCGCTTTGGTGATGGCCACTTCTGGTTCTACAGG
TACCCCGAAGGGCGCTCAGCTCACTCCGTTGAATTTGGTGAGTTCCGCCGATGCTACGCATCAGTTTTTAGGTGGCGAAG
GCCAGTGGTTGCTTGCCATGCCAGCACACCACATTGCAGGCATGCAGGTACTTATTCGAAGCCTCATTGCTGGAGTTGAG
CCACTGGCTATTGATCTCAGCACAGGTTTTCACATTGATGCGTTCGCAGACGCTGCTGCTGAATTGAAAAATACCGGTGA
CCGCGTTTACACATCTTTGACGCCAATGCAGTTACTTAAAGCAATGGACTCCTTGCAAGGCATTGAAGCCCTGAAACTTT
TTGATGTCATTCTTGTTGGCGGTTCTGCATTGTCTAAGCAGGCCCGAATTTCTGCGGAGCAGCTAGACATCAACATTGTC
ACCACCTACGGCTCCTCAGAGACTTCAGGTGGCTGCGTTTATGATGGCAAGCCCATTCCCGGCGCGAAAGTCCGTATTTC
GGATGAGCACATTGAGTTGGGTGGCCCGATGATTGCGCAGGGCTACAGAAATGCACCTGAACATCCGGATTTCGCCAACG
AGGGTTGGTTTACCACCTCTGATTCAGGTGAACTCCACGACGGGATTCTCACCGTGACTGGTCGCGTGGATACCGTCATT
GATTCCGGTGGATTGAAGTTGCACCCAGAGGTACTGGAACGTGCCATCGCAGATATTAAAGGTGTCACCGCGGCGTGTGT
TGTGGGTATTCCCGATCCCCGATTAGGCCAAGCAATTGTGGCCGCGTACTCCGGATCGATCAGTCCGTCTGAAGTTATTG
AAGGCCTCGACGATCTACCTCGTTGGCAGCTTCCCAAACGGCTGAAGCATCTGGATGCTTTGCCCAGCATTGGTCCTGGA
AAAGCTGATCGACGCGCTATCGCGAAGCTGTTTTAG

Upstream 100 bases:

>100_bases
GCAGCACTGTTTGCGCACGTCTCCTCCGGCATCTTTGTCACCAACAATGGTTGGGAATTGACCGGCGCAATCGGCGCTGG
CGCACTGCTTCTCATCGCAG

Downstream 100 bases:

>100_bases
TCTTCATTCTTGCTGGCTGCAACTAGTTTTGCCACATCTTCATCGGTGTACACTTTGGCGATCTGCTCATCATTTCCACC
CATGAGGGTGTTGCCAACAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MAQVHGASTGFWQNARPKPSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQ
LLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLIRSLIAGVE
PLAIDLSTGFHIDAFADAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGSALSKQARISAEQLDINIV
TTYGSSETSGGCVYDGKPIPGAKVRISDEHIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVI
DSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLDALPSIGPG
KADRRAIAKLF

Sequences:

>Translated_411_residues
MAQVHGASTGFWQNARPKPSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQ
LLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLIRSLIAGVE
PLAIDLSTGFHIDAFADAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGSALSKQARISAEQLDINIV
TTYGSSETSGGCVYDGKPIPGAKVRISDEHIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVI
DSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLDALPSIGPG
KADRRAIAKLF
>Mature_410_residues
AQVHGASTGFWQNARPKPSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQL
LRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLIRSLIAGVEP
LAIDLSTGFHIDAFADAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGSALSKQARISAEQLDINIVT
TYGSSETSGGCVYDGKPIPGAKVRISDEHIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVID
SGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLDALPSIGPGK
ADRRAIAKLF

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=329, Percent_Identity=29.7872340425532, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693145, Length=373, Percent_Identity=24.6648793565684, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=416, Percent_Identity=25.4807692307692, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=337, Percent_Identity=27.5964391691395, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=24.7887323943662, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653035, Length=179, Percent_Identity=31.8435754189944, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 43371; Mature: 43240

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVHGASTGFWQNARPKPSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLED
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHH
AISGKKTFLPIPVQDKTRAQLLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLV
HHCCCCEEEECCCCCHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCCCEECCHHHH
SSADATHQFLGGEGQWLLAMPAHHIAGMQVLIRSLIAGVEPLAIDLSTGFHIDAFADAAA
HCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHH
ELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGSALSKQARISAEQLDINIV
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEEEE
TTYGSSETSGGCVYDGKPIPGAKVRISDEHIELGGPMIAQGYRNAPEHPDFANEGWFTTS
EEECCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEHHCCCCCCCCCCCCCCCCEECC
DSGELHDGILTVTGRVDTVIDSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIV
CCCCCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
AAYSGSISPSEVIEGLDDLPRWQLPKRLKHLDALPSIGPGKADRRAIAKLF
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AQVHGASTGFWQNARPKPSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLED
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHH
AISGKKTFLPIPVQDKTRAQLLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLV
HHCCCCEEEECCCCCHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCCCEECCHHHH
SSADATHQFLGGEGQWLLAMPAHHIAGMQVLIRSLIAGVEPLAIDLSTGFHIDAFADAAA
HCCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHH
ELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGSALSKQARISAEQLDINIV
HHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEEEE
TTYGSSETSGGCVYDGKPIPGAKVRISDEHIELGGPMIAQGYRNAPEHPDFANEGWFTTS
EEECCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEHHCCCCCCCCCCCCCCCCEECC
DSGELHDGILTVTGRVDTVIDSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIV
CCCCCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
AAYSGSISPSEVIEGLDDLPRWQLPKRLKHLDALPSIGPGKADRRAIAKLF
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]