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The map label for this gene is folD [H]

Identifier: 138895966

GI number: 138895966

Start: 2449467

End: 2450321

Strand: Reverse

Name: folD [H]

Synonym: GTNG_2327

Alternate gene names: 138895966

Gene position: 2450321-2449467 (Counterclockwise)

Preceding gene: 138895967

Following gene: 138895965

Centisome position: 69.02

GC content: 55.09

Gene sequence:

>855_bases
ATGACAGCACAAATCATTAGCGGAACGGAATTGGCAAAAACGATTCGCGCCGAATTGGCGGATGAAGTAGCAAAGTTAAA
GGCAAACGGCATCGAACCGGGACTGGCCGTCATTCTTGTTGGCGACGACCCCGCATCGCATTCCTATGTGAAAGGAAAAC
AAAAAGCGTGTGCCGAAGTCGGCATTCGTTCACTCCTTTACACATTTCCGGCCACGATCAGCGAAGAAGAGTTGCTCGCC
AAAATTCAAGAGCTGAACGCTGATCCGACAGTGCACGGCATTTTAGTGCAGCTGCCGCTACCTGCTCATATTCGCGAGTG
GTCGGTGATTGAAACAATCGCTCCGGAAAAAGATGTCGACGGGTTTCATCCGATTAATGTTGGAAAAATGATGATCGGCC
AGCCGGCGTTTCTTCCGTGCACCCCGCACGGCGTACTGGTGATGGTCAAATCGGCCGGCATCGACATCGCCGGAAAGCAT
GTTGTCGTCGTCGGACGCAGCAATATCGTCGGCAAGCCGGTCGGCCAGCTGTTTTTGCGTGAACATGCGACCGTCACCTA
CGCTCACTCGAAAACACCGGATTTGGCCGCCATCACCCGCCAGGCTGACATTTTAATCGTTGCTGTCGGCAAGGCACGCC
TTATCGGCCCGGAACATGTGAAGCCGGGAGCGGTCGTCATTGACGTCGGCGTCAACCGACTGGAGAGCGGCAAATTGTGC
GGTGATGTCGATTTCGACGCCGTCAAAGAAGTGGCCAGCTATGTGACGCCGGTGCCGGGAGGGGTCGGGCCGATGACGAT
TACGATGCTGTTGCACAATACGATGGAAGCAGCACGGCAGCTCGCTGCGAAGTGA

Upstream 100 bases:

>100_bases
GCAAAAATAACAACCGCAAGCAGCAAACGAAGCTTCGATTTACCTAGGTGTAGGCGGACTCATATTGACCAATGAATGAG
GAGAGGGGAAGCGAGACACA

Downstream 100 bases:

>100_bases
GGGCGTTTTTTGTGGAAGTAAAATACGTGACCGTCGGAGCGCTGACAAAATACATCAAGCGCAAATTCGACGTTGATCCC
CATTTGCGCGATTTATGGAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQIISGTELAKTIRAELADEVAKLKANGIEPGLAVILVGDDPASHSYVKGKQKACAEVGIRSLLYTFPATISEEELLA
KIQELNADPTVHGILVQLPLPAHIREWSVIETIAPEKDVDGFHPINVGKMMIGQPAFLPCTPHGVLVMVKSAGIDIAGKH
VVVVGRSNIVGKPVGQLFLREHATVTYAHSKTPDLAAITRQADILIVAVGKARLIGPEHVKPGAVVIDVGVNRLESGKLC
GDVDFDAVKEVASYVTPVPGGVGPMTITMLLHNTMEAARQLAAK

Sequences:

>Translated_284_residues
MTAQIISGTELAKTIRAELADEVAKLKANGIEPGLAVILVGDDPASHSYVKGKQKACAEVGIRSLLYTFPATISEEELLA
KIQELNADPTVHGILVQLPLPAHIREWSVIETIAPEKDVDGFHPINVGKMMIGQPAFLPCTPHGVLVMVKSAGIDIAGKH
VVVVGRSNIVGKPVGQLFLREHATVTYAHSKTPDLAAITRQADILIVAVGKARLIGPEHVKPGAVVIDVGVNRLESGKLC
GDVDFDAVKEVASYVTPVPGGVGPMTITMLLHNTMEAARQLAAK
>Mature_283_residues
TAQIISGTELAKTIRAELADEVAKLKANGIEPGLAVILVGDDPASHSYVKGKQKACAEVGIRSLLYTFPATISEEELLAK
IQELNADPTVHGILVQLPLPAHIREWSVIETIAPEKDVDGFHPINVGKMMIGQPAFLPCTPHGVLVMVKSAGIDIAGKHV
VVVGRSNIVGKPVGQLFLREHATVTYAHSKTPDLAAITRQADILIVAVGKARLIGPEHVKPGAVVIDVGVNRLESGKLCG
DVDFDAVKEVASYVTPVPGGVGPMTITMLLHNTMEAARQLAAK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=46.2585034013605, Blast_Score=251, Evalue=4e-67,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=50.1694915254237, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=49.8305084745763, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI36796743, Length=218, Percent_Identity=28.8990825688073, Blast_Score=85, Evalue=6e-17,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=51.2544802867383, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=44.1780821917808, Blast_Score=227, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=45.6445993031359, Blast_Score=253, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.9463087248322, Blast_Score=233, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6322933, Length=325, Percent_Identity=26.4615384615385, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.6190476190476, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.6190476190476, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=46.551724137931, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=46.551724137931, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=46.551724137931, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=46.551724137931, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30033; Mature: 29902

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQIISGTELAKTIRAELADEVAKLKANGIEPGLAVILVGDDPASHSYVKGKQKACAEV
CCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GIRSLLYTFPATISEEELLAKIQELNADPTVHGILVQLPLPAHIREWSVIETIAPEKDVD
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHCCCHHHHHCCCCCCCC
GFHPINVGKMMIGQPAFLPCTPHGVLVMVKSAGIDIAGKHVVVVGRSNIVGKPVGQLFLR
CCCCCCCCHHEECCCCCCCCCCCCEEEEEEECCCEECCCEEEEEECCCCCCCHHHHHHHH
EHATVTYAHSKTPDLAAITRQADILIVAVGKARLIGPEHVKPGAVVIDVGVNRLESGKLC
HCCEEEEECCCCCCHHHHCCCCCEEEEEECCEEECCCCCCCCCEEEEECCCCCCCCCCCC
GDVDFDAVKEVASYVTPVPGGVGPMTITMLLHNTMEAARQLAAK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQIISGTELAKTIRAELADEVAKLKANGIEPGLAVILVGDDPASHSYVKGKQKACAEV
CCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GIRSLLYTFPATISEEELLAKIQELNADPTVHGILVQLPLPAHIREWSVIETIAPEKDVD
HHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHCCCHHHHHCCCCCCCC
GFHPINVGKMMIGQPAFLPCTPHGVLVMVKSAGIDIAGKHVVVVGRSNIVGKPVGQLFLR
CCCCCCCCHHEECCCCCCCCCCCCEEEEEEECCCEECCCEEEEEECCCCCCCHHHHHHHH
EHATVTYAHSKTPDLAAITRQADILIVAVGKARLIGPEHVKPGAVVIDVGVNRLESGKLC
HCCEEEEECCCCCCHHHHCCCCCEEEEEECCEEECCCCCCCCCEEEEECCCCCCCCCCCC
GDVDFDAVKEVASYVTPVPGGVGPMTITMLLHNTMEAARQLAAK
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA