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The map label for this gene is bfmBC [H]

Identifier: 138895949

GI number: 138895949

Start: 2430064

End: 2431485

Strand: Reverse

Name: bfmBC [H]

Synonym: GTNG_2308

Alternate gene names: 138895949

Gene position: 2431485-2430064 (Counterclockwise)

Preceding gene: 138895950

Following gene: 138895948

Centisome position: 68.49

GC content: 55.13

Gene sequence:

>1422_bases
ATGGCAAAAGAATACGATGTTGTCATTCTCGGCGGAGGCACGGGCGGCTATGTGGCGGCCATTCGCGCATCGCAGCTCGG
CTTGAAAACGGCAGTCGTCGAAAAAGGAAAGCTCGGTGGCACGTGTCTGCACGCCGGTTGCATTCCATCGAAAGCGCTGC
TCCGAAGCGCCGAAGTGTACGCACAAACGAAACAAGGTGAGACGTTCGGCGTCATCGCTGGTGATGTGCGCCTCGATTTT
GCCAAAGTGCAGGCGCGCAAAGCGGCCATTGTTGACCAACTCCATAAAGGCGTTCAGCACTTAATGAAAAAAGGAAAGAT
CGATGTGTATGCTGGGACGGGCCGTCTGCTAGGTCCATCGATCTTTTCGCCGCTGCCGGGAACGGTGTCGGTGGAAATGA
ACGACGGCAGCGAAAACGAAATGCTTGTCCCGAAATTCGTCGTTATCGCCACCGGCTCGCGGCCGCGCACGCTGCCGGGG
TTGGAGGTGGACGGCGAACTTGTCATAACGTCCGACGAGGCGCTGCAAATGGAGACGCTCCCGTCGTCCATTTTAATCGT
TGGCGGCGGGGTGATCGGCATGGAATGGGCGTCGATGTTGAATGACTTTGGGGTTGACGTCACGGTGTTAGAGTATGCAG
ACCGCATTTTGCCGACGGAAGATGAAGATGTGTCAAAAGAAATGGAAAAACTGCTCCGCCGCCGTGGCGTTACGATTGTC
ACCGGGGCGAAAGTGCTGCCGGAAACATTGGAAAAAGGAAACGGCGTGACCATTCAGGCGGAACATAACGGCGAACGGAA
AACATTCACGGCCGACAAGATGCTTGTTTCCGTCGGGCGGCAAGCAAACATTGAAGGAATCGGCCTCGAAAACACCGACA
TCGTCATTGAAAATGGCTATATTCAAACGAATGAGTTTGGTCAAACGAAAGAGGCGCATATTTACGCGATTGGCGATGTT
ATCGGTGGCTTGCAGCTCGCCCATGTCGCCGCTCATGAAGGCATCGTTGCTATCGAACATTTGGCAGGGCACAATCCGGC
GCCGATCGATTACGCGATGGTGCCACGCTGCATTTACACTCGGCCGGAAGCCGCGGCAGTTGGTTTGACGGAACAAGAAG
CGAAAGCGAAAGGCTATGACGTCAAAGTCGGCAAGTTTCCGTTTAAAGCGATTGGCAAGGCGCTTGTGTTCGGGGAAGCA
GAGGGATTCGTCAAATTCATCGCCGACCGGAATACGGACGACTTGCTTGGCGTTCATATGGTTGGGCCACATGTGACCGA
CTTGATTTCCGAAGCGGGACTCGCCCGTGTGCTTGATGCTGCTCCGTGGGAAGTGGCGCATGCGATCCATCCGCACCCGA
CACTCTCCGAAGCATTGGCAGAAGCTGCGCTTGCCGTCGATGGCCGAGCCATTCACTTTTAA

Upstream 100 bases:

>100_bases
CTTGTGTGGCGAGGAGGAGGAAAAAACGTATCTAGGTGAAGCGGCTTCGCCGGTTTTAGCCCGGCGTTGATGCGGCAACG
GACAGAAAGGGGAAGGCAAG

Downstream 100 bases:

>100_bases
TTTTGCCGCATGAGGACGAAAGATTGGCAACAGGCAGCTTACATAATGAGCTGCAATGGATTTTTAATTAAGGGAGGTTG
TTCCGATGGCGCAAAACCGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVYAQTKQGETFGVIAGDVRLDF
AKVQARKAAIVDQLHKGVQHLMKKGKIDVYAGTGRLLGPSIFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPG
LEVDGELVITSDEALQMETLPSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILPTEDEDVSKEMEKLLRRRGVTIV
TGAKVLPETLEKGNGVTIQAEHNGERKTFTADKMLVSVGRQANIEGIGLENTDIVIENGYIQTNEFGQTKEAHIYAIGDV
IGGLQLAHVAAHEGIVAIEHLAGHNPAPIDYAMVPRCIYTRPEAAAVGLTEQEAKAKGYDVKVGKFPFKAIGKALVFGEA
EGFVKFIADRNTDDLLGVHMVGPHVTDLISEAGLARVLDAAPWEVAHAIHPHPTLSEALAEAALAVDGRAIHF

Sequences:

>Translated_473_residues
MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVYAQTKQGETFGVIAGDVRLDF
AKVQARKAAIVDQLHKGVQHLMKKGKIDVYAGTGRLLGPSIFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPG
LEVDGELVITSDEALQMETLPSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILPTEDEDVSKEMEKLLRRRGVTIV
TGAKVLPETLEKGNGVTIQAEHNGERKTFTADKMLVSVGRQANIEGIGLENTDIVIENGYIQTNEFGQTKEAHIYAIGDV
IGGLQLAHVAAHEGIVAIEHLAGHNPAPIDYAMVPRCIYTRPEAAAVGLTEQEAKAKGYDVKVGKFPFKAIGKALVFGEA
EGFVKFIADRNTDDLLGVHMVGPHVTDLISEAGLARVLDAAPWEVAHAIHPHPTLSEALAEAALAVDGRAIHF
>Mature_472_residues
AKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVYAQTKQGETFGVIAGDVRLDFA
KVQARKAAIVDQLHKGVQHLMKKGKIDVYAGTGRLLGPSIFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGL
EVDGELVITSDEALQMETLPSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILPTEDEDVSKEMEKLLRRRGVTIVT
GAKVLPETLEKGNGVTIQAEHNGERKTFTADKMLVSVGRQANIEGIGLENTDIVIENGYIQTNEFGQTKEAHIYAIGDVI
GGLQLAHVAAHEGIVAIEHLAGHNPAPIDYAMVPRCIYTRPEAAAVGLTEQEAKAKGYDVKVGKFPFKAIGKALVFGEAE
GFVKFIADRNTDDLLGVHMVGPHVTDLISEAGLARVLDAAPWEVAHAIHPHPTLSEALAEAALAVDGRAIHF

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=38.7840670859539, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI50301238, Length=467, Percent_Identity=28.4796573875803, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=491, Percent_Identity=26.4765784114053, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI33519430, Length=472, Percent_Identity=24.7881355932203, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519428, Length=472, Percent_Identity=24.7881355932203, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519426, Length=472, Percent_Identity=24.7881355932203, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI148277065, Length=472, Percent_Identity=24.7881355932203, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI148277071, Length=472, Percent_Identity=24.7881355932203, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=484, Percent_Identity=26.0330578512397, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045268, Length=363, Percent_Identity=27.5482093663912, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=34.475374732334, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=28.6016949152542, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=29.0598290598291, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=26.8240343347639, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=476, Percent_Identity=40.546218487395, Blast_Score=328, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=25.5670103092783, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=499, Percent_Identity=25.6513026052104, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71983429, Length=383, Percent_Identity=26.6318537859008, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71983419, Length=383, Percent_Identity=26.6318537859008, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=485, Percent_Identity=38.5567010309278, Blast_Score=301, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=29.5218295218295, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=26.1603375527426, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=478, Percent_Identity=42.2594142259414, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=25.0513347022587, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=25.0513347022587, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=25.0513347022587, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=24.5398773006135, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50270; Mature: 50139

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY
CCCCEEEEEEECCCCCEEEEEEHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHH
AQTKQGETFGVIAGDVRLDFAKVQARKAAIVDQLHKGVQHLMKKGKIDVYAGTGRLLGPS
EECCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
IFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGLEVDGELVITSDEALQMETL
HCCCCCCEEEEEECCCCCCCEECCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECC
PSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILPTEDEDVSKEMEKLLRRRGVTIV
CCEEEEEECCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
TGAKVLPETLEKGNGVTIQAEHNGERKTFTADKMLVSVGRQANIEGIGLENTDIVIENGY
ECCHHHHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHCCCCCCCCEECCCCCEEEEECCE
IQTNEFGQTKEAHIYAIGDVIGGLQLAHVAAHEGIVAIEHLAGHNPAPIDYAMVPRCIYT
EEECCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCEEEEEHHCCCCCCCCCHHHCCHHEEC
RPEAAAVGLTEQEAKAKGYDVKVGKFPFKAIGKALVFGEAEGFVKFIADRNTDDLLGVHM
CCCHHEECCCHHHHHCCCCEEEECCCCHHHHCCEEEEECCCCCEEEEECCCCCCEEEEEE
VGPHVTDLISEAGLARVLDAAPWEVAHAIHPHPTLSEALAEAALAVDGRAIHF
CCCHHHHHHHHCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEECC
>Mature Secondary Structure 
AKEYDVVILGGGTGGYVAAIRASQLGLKTAVVEKGKLGGTCLHAGCIPSKALLRSAEVY
CCCEEEEEEECCCCCEEEEEEHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHH
AQTKQGETFGVIAGDVRLDFAKVQARKAAIVDQLHKGVQHLMKKGKIDVYAGTGRLLGPS
EECCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCH
IFSPLPGTVSVEMNDGSENEMLVPKFVVIATGSRPRTLPGLEVDGELVITSDEALQMETL
HCCCCCCEEEEEECCCCCCCEECCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEECC
PSSILIVGGGVIGMEWASMLNDFGVDVTVLEYADRILPTEDEDVSKEMEKLLRRRGVTIV
CCEEEEEECCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCEEE
TGAKVLPETLEKGNGVTIQAEHNGERKTFTADKMLVSVGRQANIEGIGLENTDIVIENGY
ECCHHHHHHHHCCCCEEEEECCCCCCEEEEHHHHHHHCCCCCCCCEECCCCCEEEEECCE
IQTNEFGQTKEAHIYAIGDVIGGLQLAHVAAHEGIVAIEHLAGHNPAPIDYAMVPRCIYT
EEECCCCCCCCEEEEEEHHHHCCHHHHHHHHHCCEEEEEHHCCCCCCCCCHHHCCHHEEC
RPEAAAVGLTEQEAKAKGYDVKVGKFPFKAIGKALVFGEAEGFVKFIADRNTDDLLGVHM
CCCHHEECCCHHHHHCCCCEEEECCCCHHHHCCEEEEECCCCCEEEEECCCCCCEEEEEE
VGPHVTDLISEAGLARVLDAAPWEVAHAIHPHPTLSEALAEAALAVDGRAIHF
CCCHHHHHHHHCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]