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The map label for this gene is gudB [H]

Identifier: 138895812

GI number: 138895812

Start: 2298936

End: 2300207

Strand: Reverse

Name: gudB [H]

Synonym: GTNG_2171

Alternate gene names: 138895812

Gene position: 2300207-2298936 (Counterclockwise)

Preceding gene: 138895813

Following gene: 138895811

Centisome position: 64.79

GC content: 53.62

Gene sequence:

>1272_bases
ATGGCAGCCGATAAGCATACGGAAGACAAAGAACAACAGTACGACGTGTTGGCGTCGACGCAAATTGTTATACATAGAGC
ATTGGAAAAGCTCGGCTACCCGGAAGAAGTATACGAGCTCCTGAAAGAGCCGATCCGCGTGCTGACCGTCCGCATTCCGG
TGCGCATGGACGACGGATCGGTGAAAATTTTTACTGGCTATCGGGCACAGCATAACGATGCGGTCGGTCCGACAAAAGGT
GGCGTGCGCTTCCATCCTAATGTCACTGAACGTGAGGTGAAAGCGCTGTCTATTTGGATGACCTTAAAATGCGGTATCGT
CGATCTGCCGTATGGCGGCGGCAAAGGCGGTATCGTCTGTGATCCGCGCACGATGTCGTTCCGCGAGCTTGAACGGTTGA
GCCGCGGCTATGTGCGCGCCATCAGCCAAATCGTTGGACCATCCAAAGACATTCCAGCACCTGATGTGTTTACGAACTCG
CAAATTATGGCGTGGATGATGGATGAGTATAGCCGCATCCGTGAATTCGATTCGCCGGGCTTTATTACCGGAAAGCCACT
CGTGCTCGGCGGTTCGCATGGTCGGGAAACAGCGACGGCCAAAGGGGTGACGATTTGTATCCGCGAGGCGGCGAAAAAAC
GCGGCTTGTCGCTCGAAGGAGCGCGTGTCGTCGTTCAAGGGTTTGGCAACGCCGGCAGCTATTTGGCGAAGTTTATGCAT
GACGCCGGTGCGAAAGTCGTTGGTATTTCCGACGTATACGGTGCGTTGTACGATCCGAACGGGCTTGATATCGACTATTT
GCTCGAGCGGCGTGACAGCTTTGGCACGGTGACGAAGCTGTTTAAAAATACGATTTCCAATAAAGAACTGCTTGAACTCG
ATTGCGACATTTTAGTGCCGGCGGCTATCGAAAACCAAATTACGGCCGAAAATGCCCCACGCATTAAGGCGAGCATCGTC
GTTGAGGCGGCGAACGGCCCGACGACGCTCGAGGCAACCGAAATTTTAACGCAACGCGGCATTTTGCTCGTCCCGGATGT
GCTCGCAAGCGCTGGTGGCGTGACAGTGTCGTACTTTGAATGGGTGCAAAACAACCAAGGCTATTATTGGACGGAAGAAG
AAGTCGAACAGCGGCTTGAGAAAGTGATGGTCAAGGCGTTTAATAATGTTTATGACATGGCGCAAACGCGCCGCGTTGAT
ATGCGCCTTGCCGCATACATGGTCGGCGTCCGTAAAATGGCGGAAGCGTGCCGATTCCGCGGCTGGGTGTAA

Upstream 100 bases:

>100_bases
CCGTTTCAGCCTTTTTTCCTTTGCATCCGCCATCAGAAGGTGTATACTATGACATGGAGGGCGACAAACTATTATAGCGA
GCTTATAGGAGGTTTACGTG

Downstream 100 bases:

>100_bases
ACGCGGCGAGCACAGACGTTTGCATGCTGGAGTGAAATCCCCTATCATAAGATAGGGGATTTTTTGCTTCGTGTGAGGTG
ACAAAAAACATGAGAGAAGA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAADKHTEDKEQQYDVLASTQIVIHRALEKLGYPEEVYELLKEPIRVLTVRIPVRMDDGSVKIFTGYRAQHNDAVGPTKG
GVRFHPNVTEREVKALSIWMTLKCGIVDLPYGGGKGGIVCDPRTMSFRELERLSRGYVRAISQIVGPSKDIPAPDVFTNS
QIMAWMMDEYSRIREFDSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGLSLEGARVVVQGFGNAGSYLAKFMH
DAGAKVVGISDVYGALYDPNGLDIDYLLERRDSFGTVTKLFKNTISNKELLELDCDILVPAAIENQITAENAPRIKASIV
VEAANGPTTLEATEILTQRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVKAFNNVYDMAQTRRVD
MRLAAYMVGVRKMAEACRFRGWV

Sequences:

>Translated_423_residues
MAADKHTEDKEQQYDVLASTQIVIHRALEKLGYPEEVYELLKEPIRVLTVRIPVRMDDGSVKIFTGYRAQHNDAVGPTKG
GVRFHPNVTEREVKALSIWMTLKCGIVDLPYGGGKGGIVCDPRTMSFRELERLSRGYVRAISQIVGPSKDIPAPDVFTNS
QIMAWMMDEYSRIREFDSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGLSLEGARVVVQGFGNAGSYLAKFMH
DAGAKVVGISDVYGALYDPNGLDIDYLLERRDSFGTVTKLFKNTISNKELLELDCDILVPAAIENQITAENAPRIKASIV
VEAANGPTTLEATEILTQRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVKAFNNVYDMAQTRRVD
MRLAAYMVGVRKMAEACRFRGWV
>Mature_422_residues
AADKHTEDKEQQYDVLASTQIVIHRALEKLGYPEEVYELLKEPIRVLTVRIPVRMDDGSVKIFTGYRAQHNDAVGPTKGG
VRFHPNVTEREVKALSIWMTLKCGIVDLPYGGGKGGIVCDPRTMSFRELERLSRGYVRAISQIVGPSKDIPAPDVFTNSQ
IMAWMMDEYSRIREFDSPGFITGKPLVLGGSHGRETATAKGVTICIREAAKKRGLSLEGARVVVQGFGNAGSYLAKFMHD
AGAKVVGISDVYGALYDPNGLDIDYLLERRDSFGTVTKLFKNTISNKELLELDCDILVPAAIENQITAENAPRIKASIVV
EAANGPTTLEATEILTQRGILLVPDVLASAGGVTVSYFEWVQNNQGYYWTEEEVEQRLEKVMVKAFNNVYDMAQTRRVDM
RLAAYMVGVRKMAEACRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=41.6422287390029, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4885281, Length=341, Percent_Identity=40.7624633431085, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1788059, Length=400, Percent_Identity=32, Blast_Score=182, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17544676, Length=335, Percent_Identity=42.3880597014925, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6319256, Length=415, Percent_Identity=29.8795180722892, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=408, Percent_Identity=28.921568627451, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45549226, Length=341, Percent_Identity=40.4692082111437, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI45553475, Length=341, Percent_Identity=40.4692082111437, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24649283, Length=328, Percent_Identity=40.8536585365854, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24649460, Length=280, Percent_Identity=39.6428571428571, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46937; Mature: 46806

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADKHTEDKEQQYDVLASTQIVIHRALEKLGYPEEVYELLKEPIRVLTVRIPVRMDDGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCCC
VKIFTGYRAQHNDAVGPTKGGVRFHPNVTEREVKALSIWMTLKCGIVDLPYGGGKGGIVC
EEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEEECEEECCCCCCCCCEEE
DPRTMSFRELERLSRGYVRAISQIVGPSKDIPAPDVFTNSQIMAWMMDEYSRIREFDSPG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
FITGKPLVLGGSHGRETATAKGVTICIREAAKKRGLSLEGARVVVQGFGNAGSYLAKFMH
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHH
DAGAKVVGISDVYGALYDPNGLDIDYLLERRDSFGTVTKLFKNTISNKELLELDCDILVP
HCCCEEEEHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCEEEC
AAIENQITAENAPRIKASIVVEAANGPTTLEATEILTQRGILLVPDVLASAGGVTVSYFE
HHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEHH
WVQNNQGYYWTEEEVEQRLEKVMVKAFNNVYDMAQTRRVDMRLAAYMVGVRKMAEACRFR
HHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWV
CCC
>Mature Secondary Structure 
AADKHTEDKEQQYDVLASTQIVIHRALEKLGYPEEVYELLKEPIRVLTVRIPVRMDDGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEEEECCCC
VKIFTGYRAQHNDAVGPTKGGVRFHPNVTEREVKALSIWMTLKCGIVDLPYGGGKGGIVC
EEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHEEEEEEEEECEEECCCCCCCCCEEE
DPRTMSFRELERLSRGYVRAISQIVGPSKDIPAPDVFTNSQIMAWMMDEYSRIREFDSPG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
FITGKPLVLGGSHGRETATAKGVTICIREAAKKRGLSLEGARVVVQGFGNAGSYLAKFMH
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHH
DAGAKVVGISDVYGALYDPNGLDIDYLLERRDSFGTVTKLFKNTISNKELLELDCDILVP
HCCCEEEEHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCEEEC
AAIENQITAENAPRIKASIVVEAANGPTTLEATEILTQRGILLVPDVLASAGGVTVSYFE
HHHCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCEEECHHHHCCCCCEEEEHH
WVQNNQGYYWTEEEVEQRLEKVMVKAFNNVYDMAQTRRVDMRLAAYMVGVRKMAEACRFR
HHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]