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The map label for this gene is trpA

Identifier: 138895777

GI number: 138895777

Start: 2264522

End: 2265328

Strand: Reverse

Name: trpA

Synonym: GTNG_2133

Alternate gene names: 138895777

Gene position: 2265328-2264522 (Counterclockwise)

Preceding gene: 138895778

Following gene: 138895776

Centisome position: 63.81

GC content: 55.39

Gene sequence:

>807_bases
TTGCGTCTGCCCGTTAACCGATCGTTATTTATTCCGTTTATTGTTGCCGGCGACCCGTCTGCTGATTTGACTGTTGACCT
CGCTTTGGCGCTGCAAGACGCCGGTGCTGACGTGTTGGAGCTTGGCGTTCCGTACTCTGACCCACTCGCTGATGGGCCGA
CGATCCAGCGCGCCGCCGCCCGGGCGCTCGCCGGACAAATGACGCTGCCGAAAGCCATTCAACTTGTTGCCGACATGCGA
AAAAAAGGAGTAAAAATTCCGATTATTATCTTTACGTATTATAATCCTGTGTTACAATTAGGAGAAGAATCCTTTTTTGC
TTTAGCGCAAGAAAATGGCGCCGACGGCGTGCTCATTCCCGACTTGCCGTTTGAGGAGAGCGGCCCGCTCCTCGAGCTCA
GTGAGCGGTTCGGACTTCCGCTTATTTCGCTTGTCGCTCCGACATCAAAGCAGCGAATTGAGCAGATTGCTTCAGCGGCG
CAGGGGTTTTTGTACTGTGTCTCCTCGCTTGGCGTCACGGGCGTGCGTGAGACACTGCCGGAGTCGCTTGGCCATTTCCT
AGGTGAAGTAAAGCGGCATAGCCGTGTCCCGGTCGTTGTCGGTTTCGGCATTTCAACACCGGAACAAGTGGCGATGCTGA
AAGACGCCTGTGATGGCGTCGTGGTCGGCAGTGCGCTCGTGCAAAAAATCGAACAGTTGCTTGAGCGCCTGCAGACCTTG
GAAGAAAAGAATGCGGCTATCGCTGAATTTGCCTCCTATGCCCGTTCGCTCGCCGCGCCGCTTCGGGAGCCGTGTTCTTC
GCGCTAA

Upstream 100 bases:

>100_bases
ATGACGCCGGATGAGACGGTGCTTATTTGCCTGTCCGGCCGCGGCGATAAAGATGTGCAAATGATGATGCACCATCTTGG
GGTGAAAGAAGGTGAAGACG

Downstream 100 bases:

>100_bases
AAAGTTCCGACTTAAAGGAGTGGGCGATATCCATGCAAGTAAAGGAGCAACTTCGGGGACTCCCCCCGTACCAACCGGGA
AAATCGATTGAGGAAGTGAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRLPVNRSLFIPFIVAGDPSADLTVDLALALQDAGADVLELGVPYSDPLADGPTIQRAAARALAGQMTLPKAIQLVADMR
KKGVKIPIIIFTYYNPVLQLGEESFFALAQENGADGVLIPDLPFEESGPLLELSERFGLPLISLVAPTSKQRIEQIASAA
QGFLYCVSSLGVTGVRETLPESLGHFLGEVKRHSRVPVVVGFGISTPEQVAMLKDACDGVVVGSALVQKIEQLLERLQTL
EEKNAAIAEFASYARSLAAPLREPCSSR

Sequences:

>Translated_268_residues
MRLPVNRSLFIPFIVAGDPSADLTVDLALALQDAGADVLELGVPYSDPLADGPTIQRAAARALAGQMTLPKAIQLVADMR
KKGVKIPIIIFTYYNPVLQLGEESFFALAQENGADGVLIPDLPFEESGPLLELSERFGLPLISLVAPTSKQRIEQIASAA
QGFLYCVSSLGVTGVRETLPESLGHFLGEVKRHSRVPVVVGFGISTPEQVAMLKDACDGVVVGSALVQKIEQLLERLQTL
EEKNAAIAEFASYARSLAAPLREPCSSR
>Mature_268_residues
MRLPVNRSLFIPFIVAGDPSADLTVDLALALQDAGADVLELGVPYSDPLADGPTIQRAAARALAGQMTLPKAIQLVADMR
KKGVKIPIIIFTYYNPVLQLGEESFFALAQENGADGVLIPDLPFEESGPLLELSERFGLPLISLVAPTSKQRIEQIASAA
QGFLYCVSSLGVTGVRETLPESLGHFLGEVKRHSRVPVVVGFGISTPEQVAMLKDACDGVVVGSALVQKIEQLLERLQTL
EEKNAAIAEFASYARSLAAPLREPCSSR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=233, Percent_Identity=38.1974248927039, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=224, Percent_Identity=41.9642857142857, Blast_Score=186, Evalue=3e-48,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_GEOTN (A4IQ81)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001126230.1
- ProteinModelPortal:   A4IQ81
- SMR:   A4IQ81
- STRING:   A4IQ81
- GeneID:   4967061
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_2133
- NMPDR:   fig|420246.5.peg.2040
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A4IQ81
- ProtClustDB:   PRK13111
- BioCyc:   GTHE420246:GTNG_2133-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28664; Mature: 28664

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 40-40 ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPVNRSLFIPFIVAGDPSADLTVDLALALQDAGADVLELGVPYSDPLADGPTIQRAAA
CCCCCCCCEEEEEEEECCCCCCEEEEHEEHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHH
RALAGQMTLPKAIQLVADMRKKGVKIPIIIFTYYNPVLQLGEESFFALAQENGADGVLIP
HHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHCCHHHHHHHHCCCCCCEEEC
DLPFEESGPLLELSERFGLPLISLVAPTSKQRIEQIASAAQGFLYCVSSLGVTGVRETLP
CCCCCCCCCCEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ESLGHFLGEVKRHSRVPVVVGFGISTPEQVAMLKDACDGVVVGSALVQKIEQLLERLQTL
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EEKNAAIAEFASYARSLAAPLREPCSSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLPVNRSLFIPFIVAGDPSADLTVDLALALQDAGADVLELGVPYSDPLADGPTIQRAAA
CCCCCCCCEEEEEEEECCCCCCEEEEHEEHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHH
RALAGQMTLPKAIQLVADMRKKGVKIPIIIFTYYNPVLQLGEESFFALAQENGADGVLIP
HHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHCCHHHHHHHHCCCCCCEEEC
DLPFEESGPLLELSERFGLPLISLVAPTSKQRIEQIASAAQGFLYCVSSLGVTGVRETLP
CCCCCCCCCCEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ESLGHFLGEVKRHSRVPVVVGFGISTPEQVAMLKDACDGVVVGSALVQKIEQLLERLQTL
HHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EEKNAAIAEFASYARSLAAPLREPCSSR
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA