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The map label for this gene is yaaO [H]

Identifier: 138893701

GI number: 138893701

Start: 35262

End: 36698

Strand: Direct

Name: yaaO [H]

Synonym: GTNG_0022

Alternate gene names: 138893701

Gene position: 35262-36698 (Clockwise)

Preceding gene: 138893700

Following gene: 138893702

Centisome position: 0.99

GC content: 50.52

Gene sequence:

>1437_bases
ATGAATCAAGCGAAAACCCCGTTATATGACGCGTTAACGCGCCATTGGGCACGCCGTCCAGTTTCGTTCCATGTGCCGGG
CCATAAATATGGGGCCATATTTCCGAAACGGGCGGAGGATGTATTTTCGTCATTATTGGCGTTGGATGTGACGGAGATTT
CGGGACTTGACGATTTGCACCATCCGGAATCAGTCATTGCTGAGGCACAAGCATTGGCAGCTGAACTTTATGGTGCTGAG
AAAACGTTTTTCCTTGTAAACGGATCGACGTCCGGAAATTTGGCAATGATTGCTGCCGTATGTGATGAAAAGAAGCGAAA
AGTCATTGTACAGCGAAACTGCCATAAATCTGTGATGCATGCACTGCAGTTAATGGGAGCAACCCCTGTTCTTCTTTCCC
CTGAGTTTGACAGTGATATGCGTGTTGCTTCGCACGTTTGCATGGAGACTGTCAAAGAGGCGCTTGCCCTTCATCGAGAC
GCTGCCGCCCTCGTATTGACGAATCCGAATTATTATGGCATGTCAATTGATTTAACGGAAATCGTCCGTCTAGCTCACGA
CCATGGGATCCCCGTGTTAGTCGATGAAGCGCATGGCGCCCATTTTGTTGTTGGTCCTCCGTTTCCGAAGTCAGCGCTTG
CCTGCGGTGCCGATATGGTCGTGCAGTCGGCTCATAAAACGCTGCCGGCAATGACGATGGGGGCATTTTTACATGTGAAC
AGTGGGCGTATCGATGTAGAGCGGTTGAAATATTTTTTGCAGCTGTTTCAATCGAGCAGCCCGTCGTACCCGATTATGGC
GTCGCTTGATTTAGCTCGCAGCTATGTTGCTAAGCTTATAGAAGATGGTGTGGAGGCGATTGTGGAGAAAATTGAAGAGT
TCAAGACGGCGTTGAATGATATTGCTGGGGTGACGGTTGTTTCTTCCCGACATCCTGATGTGCAAATCGATCCGCTAAAA
GTGACGGTGCAAACGCGCAGTTCGCTGACAGGGTATGAACTACAGCATCGGCTTGAACGCGAAGGGGTGTTTGCGGAACT
GGCCGATCCGTTGAACGTTCTACTCGTTTGTCCACTTGCTGCGAACGGACAGCTTATAGAGACAGTGGACAAAATGAAAC
GGGCGTGGTGCGGGTTGCCGACTGGTCAGGTACCGCTGTTTGGCTCTTTCCCGTTGTTACGCTCGCCGTTGTCAGTATTA
GTTCCGTATGATGAGCTGCGTGATGTACGGAAAAAAGTGGTCGCTTTAGAGGAGGCAGAAGGGTATGTGGCGGCGGAAAC
GGTTATTCCATATCCACCTGGGGTGCCGCTTATGTGGATTGGGGAACGGATCGACCGCAGCCATATTGAACAAATTCGCC
AATTCCTCCACCATCAGGCGCATATCCAAGGCGGAAGCCGGCTCAGGGATGGGTTGCTTGTCGTATACGAACTTTGA

Upstream 100 bases:

>100_bases
TTGTTGTTTCCCGTTACATATCTAATGGTTTCCCATTTTTATTCAGATATCTGTCGTGTTTCTTATGTATGCAAAAGAGC
AACAAGGAAAGAGGATTACA

Downstream 100 bases:

>100_bases
TGATGGAAAGGATGCTACGGGCAATGAACGGGTATTTTTTTTCGTTTGAAGGGCCGGAAGGGGCCGGAAAAACAACGATG
ATTGGCAAGCTTGAGTCATT

Product: lysine decarboxylase-like protein

Products: 1,5-Diaminopentane; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNQAKTPLYDALTRHWARRPVSFHVPGHKYGAIFPKRAEDVFSSLLALDVTEISGLDDLHHPESVIAEAQALAAELYGAE
KTFFLVNGSTSGNLAMIAAVCDEKKRKVIVQRNCHKSVMHALQLMGATPVLLSPEFDSDMRVASHVCMETVKEALALHRD
AAALVLTNPNYYGMSIDLTEIVRLAHDHGIPVLVDEAHGAHFVVGPPFPKSALACGADMVVQSAHKTLPAMTMGAFLHVN
SGRIDVERLKYFLQLFQSSSPSYPIMASLDLARSYVAKLIEDGVEAIVEKIEEFKTALNDIAGVTVVSSRHPDVQIDPLK
VTVQTRSSLTGYELQHRLEREGVFAELADPLNVLLVCPLAANGQLIETVDKMKRAWCGLPTGQVPLFGSFPLLRSPLSVL
VPYDELRDVRKKVVALEEAEGYVAAETVIPYPPGVPLMWIGERIDRSHIEQIRQFLHHQAHIQGGSRLRDGLLVVYEL

Sequences:

>Translated_478_residues
MNQAKTPLYDALTRHWARRPVSFHVPGHKYGAIFPKRAEDVFSSLLALDVTEISGLDDLHHPESVIAEAQALAAELYGAE
KTFFLVNGSTSGNLAMIAAVCDEKKRKVIVQRNCHKSVMHALQLMGATPVLLSPEFDSDMRVASHVCMETVKEALALHRD
AAALVLTNPNYYGMSIDLTEIVRLAHDHGIPVLVDEAHGAHFVVGPPFPKSALACGADMVVQSAHKTLPAMTMGAFLHVN
SGRIDVERLKYFLQLFQSSSPSYPIMASLDLARSYVAKLIEDGVEAIVEKIEEFKTALNDIAGVTVVSSRHPDVQIDPLK
VTVQTRSSLTGYELQHRLEREGVFAELADPLNVLLVCPLAANGQLIETVDKMKRAWCGLPTGQVPLFGSFPLLRSPLSVL
VPYDELRDVRKKVVALEEAEGYVAAETVIPYPPGVPLMWIGERIDRSHIEQIRQFLHHQAHIQGGSRLRDGLLVVYEL
>Mature_478_residues
MNQAKTPLYDALTRHWARRPVSFHVPGHKYGAIFPKRAEDVFSSLLALDVTEISGLDDLHHPESVIAEAQALAAELYGAE
KTFFLVNGSTSGNLAMIAAVCDEKKRKVIVQRNCHKSVMHALQLMGATPVLLSPEFDSDMRVASHVCMETVKEALALHRD
AAALVLTNPNYYGMSIDLTEIVRLAHDHGIPVLVDEAHGAHFVVGPPFPKSALACGADMVVQSAHKTLPAMTMGAFLHVN
SGRIDVERLKYFLQLFQSSSPSYPIMASLDLARSYVAKLIEDGVEAIVEKIEEFKTALNDIAGVTVVSSRHPDVQIDPLK
VTVQTRSSLTGYELQHRLEREGVFAELADPLNVLLVCPLAANGQLIETVDKMKRAWCGLPTGQVPLFGSFPLLRSPLSVL
VPYDELRDVRKKVVALEEAEGYVAAETVIPYPPGVPLMWIGERIDRSHIEQIRQFLHHQAHIQGGSRLRDGLLVVYEL

Specific function: Ldc Is Constitutively But Weakly Expressed Under Various Conditions. [C]

COG id: COG1982

COG function: function code E; Arginine/lysine/ornithine decarboxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-I family [H]

Homologues:

Organism=Escherichia coli, GI1786384, Length=551, Percent_Identity=22.3230490018149, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI221142684, Length=421, Percent_Identity=24.7030878859857, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082193, Length=327, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000310
- InterPro:   IPR008286
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF01276 OKR_DC_1; PF03711 OKR_DC_1_C [H]

EC number: 4.1.1.18 [C]

Molecular weight: Translated: 52643; Mature: 52643

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAKTPLYDALTRHWARRPVSFHVPGHKYGAIFPKRAEDVFSSLLALDVTEISGLDDLH
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
HPESVIAEAQALAAELYGAEKTFFLVNGSTSGNLAMIAAVCDEKKRKVIVQRNCHKSVMH
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEHHCCHHHEEHHHHHHHHHHHH
ALQLMGATPVLLSPEFDSDMRVASHVCMETVKEALALHRDAAALVLTNPNYYGMSIDLTE
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECHHH
IVRLAHDHGIPVLVDEAHGAHFVVGPPFPKSALACGADMVVQSAHKTLPAMTMGAFLHVN
HHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHCHHHHHHHHHHHCCHHHHCEEEEEC
SGRIDVERLKYFLQLFQSSSPSYPIMASLDLARSYVAKLIEDGVEAIVEKIEEFKTALND
CCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGVTVVSSRHPDVQIDPLKVTVQTRSSLTGYELQHRLEREGVFAELADPLNVLLVCPLA
HCCEEEEECCCCCEEECCEEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCC
ANGQLIETVDKMKRAWCGLPTGQVPLFGSFPLLRSPLSVLVPYDELRDVRKKVVALEEAE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHCC
GYVAAETVIPYPPGVPLMWIGERIDRSHIEQIRQFLHHQAHIQGGSRLRDGLLVVYEL
CEEEEEEECCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MNQAKTPLYDALTRHWARRPVSFHVPGHKYGAIFPKRAEDVFSSLLALDVTEISGLDDLH
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
HPESVIAEAQALAAELYGAEKTFFLVNGSTSGNLAMIAAVCDEKKRKVIVQRNCHKSVMH
CHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEHHCCHHHEEHHHHHHHHHHHH
ALQLMGATPVLLSPEFDSDMRVASHVCMETVKEALALHRDAAALVLTNPNYYGMSIDLTE
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECHHH
IVRLAHDHGIPVLVDEAHGAHFVVGPPFPKSALACGADMVVQSAHKTLPAMTMGAFLHVN
HHHHHHCCCCEEEEECCCCCEEEECCCCCHHHHHHCHHHHHHHHHHHCCHHHHCEEEEEC
SGRIDVERLKYFLQLFQSSSPSYPIMASLDLARSYVAKLIEDGVEAIVEKIEEFKTALND
CCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGVTVVSSRHPDVQIDPLKVTVQTRSSLTGYELQHRLEREGVFAELADPLNVLLVCPLA
HCCEEEEECCCCCEEECCEEEEEECCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCC
ANGQLIETVDKMKRAWCGLPTGQVPLFGSFPLLRSPLSVLVPYDELRDVRKKVVALEEAE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHCCCHHHHHHHHHHHHHHHHCC
GYVAAETVIPYPPGVPLMWIGERIDRSHIEQIRQFLHHQAHIQGGSRLRDGLLVVYEL
CEEEEEEECCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate; Sulfhydryl groups [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.4 {S-aminoethyl-L-Cys}} 2 {L-Lys}} 1.5 {L-Lys}} 7 {delta-hydroxylysine}} [C]

Substrates: H+; L-Lysine [C]

Specific reaction: H+ + L-Lysine --> 1,5-Diaminopentane + CO2 [C]

General reaction: Decarboxylation [C]

Inhibitor: NaBH4; NaCl [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]