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The map label for this gene is gatB [H]

Identifier: 13507977

GI number: 13507977

Start: 290256

End: 291692

Strand: Direct

Name: gatB [H]

Synonym: MPN238

Alternate gene names: 13507977

Gene position: 290256-291692 (Clockwise)

Preceding gene: 13507976

Following gene: 13507978

Centisome position: 35.55

GC content: 41.27

Gene sequence:

>1437_bases
ATGATGATTAACTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGC
GCCCAACCAAGCGCAAAACGCCACACCAAACCAGTTCATAAACGCGATTGATTTGGGCTTGCCTGGTACCATGCCCCAGG
TCAATGAGCAGGCGGTACAAAAGGCGTTAATCCTTGCTGACGCGCTCAACATGCAACGGGTACAGCCAGTCTTAGTGTTT
GACCGTAAGCACTACTTTTACCAAGATCTACCTAAGGGTTTTCAGATTACCCAGCAAAACTTTCCGATTGCGCAAAACGG
TTACGTTGAAATTGTGGAAAACAACAAAGCGCAGCGGATTATCATTGAACGGTTTCACCTAGAGGAAGATACTGCCAAGC
AACATTTTGTCGATGGGCAAATTCTGCTCGACTTTAACCGTTGTGGTGCACCGCTAATTGAAGTAGTTACCGCGCCCGTG
ATTAAGAGTGCTAAGCAGTCTAAGGCATACTTGCAAAAGCTACGTCAAATTTTGATTGTTAACAACATTTCCAACGCTAA
GCTCGAAGATGGCTCGATGCGCAGTGACTGTAATGTATCAGTGCGCTTAAAGGGACAAACTGCTTTTGGCACCAAGGTCG
AAATTAAGAACATTAACTCACTCAATAATGTGGAAAAGGCAATTGATTTGGAAATAGCACGTCAAGTCAAAGCACTAATT
AAAGGCGAACCGGTTCAACAAGTTACCCTAACTTATGACGACAAAACCAATACCAATGTCTTCATGCGTAAAAAGGACAA
TTCAGTCGATTACCGTTATTTCATTGAACCCAACATTATGAGCAGTAACATTGATGAACTGCTACAAAAACCCAACAAGG
CCTTTAACTTAACTGAGTTCTTTACCAAGCTCAAGGAAGCTGGGGTTAATGAGCAGCTAAATCAATTAGTAGTCGATGAT
CTGACTCTTTTTAATGCTTACACCAAGATTAACAGTGTCATTAACAGTCCCCAAGATACCATTCGCTGGTTGTGCATTGA
GTTAATGGGACAACTCAACAAGCTACAAAAACCGCTCAAGGACAAAACGATTGATCACTTAATTATCCTAATCCAAATGG
TACAGAAGGGCACCGTGAACCAAAAACAAGCAAAGCAGTTAATTGAGTTAATGTTAGACAATGGGCAAGACCCCCAATCG
CTGGCTAAACTACACAACTTAGAACAGATAACTGATGAAAAACAACTAACGCAAATCATCCAACAAATCTTTAAGGAAAA
CGAGGGTGAAATCCTTAAGAACTTAGATCGAGTTGAACGAATTCAAAAGCTAATTATAGGTCAAGTTATGCAAAGAACCC
ACAATCGCGCCAATCCCCAGCAAGTTTTTATAATTGTTGAAAAGTTGCTTCATGATTTCAGCGAAAGAGCAAGCTAA

Upstream 100 bases:

>100_bases
AAGGACCAAACGATTGGTTTGAGTGTTAATGCTAACTGCTTTCACGACAAGCAATTATTACAAGTAGCTGCTTGATTGGA
GGAATTATTCCAAATTGAAC

Downstream 100 bases:

>100_bases
AAAAGGTCAAATAATTTACCGTTACATTCTCCTTAAAATTCAAAGCTTTAAATGGCCTGCTGGCACCCGCATCTTCTCTG
AACGCCAGCTCGAAATTCGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVF
DRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPV
IKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALI
KGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDD
LTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQS
LAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS

Sequences:

>Translated_478_residues
MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVF
DRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPV
IKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALI
KGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDD
LTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQS
LAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS
>Mature_478_residues
MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQKALILADALNMQRVQPVLVF
DRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRIIIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPV
IKSAKQSKAYLQKLRQILIVNNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALI
KGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEFFTKLKEAGVNEQLNQLVVDD
LTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLKDKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQS
LAKLHNLEQITDEKQLTQIIQQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=29.5180722891566, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI115534094, Length=286, Percent_Identity=39.1608391608392, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=28.4, Blast_Score=176, Evalue=9e-45,
Organism=Drosophila melanogaster, GI45550808, Length=503, Percent_Identity=28.8270377733598, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54563; Mature: 54563

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQ
CEEEEEEEEEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
KALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRI
HHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCEE
IIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIV
HHHHHCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALI
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEF
CCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHH
FTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQII
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
QQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMINFEAIIGIEVHVVLNTATKMFSPAPNQAQNATPNQFINAIDLGLPGTMPQVNEQAVQ
CEEEEEEEEEEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHH
KALILADALNMQRVQPVLVFDRKHYFYQDLPKGFQITQQNFPIAQNGYVEIVENNKAQRI
HHHHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCEE
IIERFHLEEDTAKQHFVDGQILLDFNRCGAPLIEVVTAPVIKSAKQSKAYLQKLRQILIV
HHHHHCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNISNAKLEDGSMRSDCNVSVRLKGQTAFGTKVEIKNINSLNNVEKAIDLEIARQVKALI
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHH
KGEPVQQVTLTYDDKTNTNVFMRKKDNSVDYRYFIEPNIMSSNIDELLQKPNKAFNLTEF
CCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHH
FTKLKEAGVNEQLNQLVVDDLTLFNAYTKINSVINSPQDTIRWLCIELMGQLNKLQKPLK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DKTIDHLIILIQMVQKGTVNQKQAKQLIELMLDNGQDPQSLAKLHNLEQITDEKQLTQII
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
QQIFKENEGEILKNLDRVERIQKLIIGQVMQRTHNRANPQQVFIIVEKLLHDFSERAS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]