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The map label for this gene is gatA [H]

Identifier: 13507976

GI number: 13507976

Start: 288830

End: 290266

Strand: Direct

Name: gatA [H]

Synonym: MPN237

Alternate gene names: 13507976

Gene position: 288830-290266 (Clockwise)

Preceding gene: 13507975

Following gene: 13507977

Centisome position: 35.38

GC content: 41.89

Gene sequence:

>1437_bases
GTGAAATCGCAAATCTTAAAGCTCCAGCAAACACTAACTAAAAAACCAGCGTCAATCAATCCGTTATTACAACAAATTGA
TGGAGCAATTAACGAACACTGATCGAGTAACTTTTTGTTGAAAAATACGGTTGAGTGAGCACAAGCCCAAGCACCCAAAA
ACCGCAGTAAATCACCACTCAACAACATTCCCTTTGTTTTAAAGGACAACATTGCCACGAAGGGAATTGTTACTACTGGT
GGTTCGAGGTTTTTGGAAGACTATATCCCGCCGTTTTCAGCAACTGTGTTTGAATTGCTAAACAACAGTGGCGCTTTATT
AGTTGGTAAAGCGAACCTCGACGAGTTTGGTTTAGGCGGTACGGGGCTCCATTCGGGTTTTGGTTTTGTCCACCACCCTT
GAAACGAAACCCTAATTCCTGGTGGTTCTTCATCCGGTTCCGCTTATGCAGTGGCGCGCGGCATTGTTCCCTTTTCAATT
GGTACGGACACGGGAGATTCGGTACGCCGTCCAGCTAGCATTTGTAACATTGTTGGTTTTAAGCCCACCTATGGTTTGAT
ATCGCGGAACGGAGTGTATCCTTACGCGCCTAGTTTAGACCATGTCGGGATCTTTGCGCGGTATGTGTATGATGTAGCAC
TTGTTAGTGATGAAATCATTAAACATGACAAAGCTGATTTTTCCGCTCAAAAATCTCCTGATGCTGGCAAGTTTACCAGG
TCCTTAAAAGAGTCCTTCAACAAGCAAATCAAGATAGGTTATCTAAAACCGCTAGAGGAATGGTTTGACATTGAACTGAG
CAAAAAGTGAAACAGCTTAAAGGAAAGAATTACCTTGGAGGGTTGTGAGTTAATTCCGTTTCACTTTCCGCTTGAACTGC
TAGAGGTAATTGATCCAGTTTACAAGCTGATTAGTTACAGTGAAGCGGTGAGCTGTTACAGTAATCTCACTGGGATTGTC
TTTGGTCAAAAATTGTTTGAACCCAACCAAGCGAGTGACTTTTCCAAAACAATTACTGCCAACCGCGACCGTTTCTTTGG
TGAGCAGTTAAAACGCCGCTTTATTATTGGTGCTTTTGGTACTGACAAGAATAACTTTACCAAGTACTTTGAAAAGGCTC
AAAAGATCCGCCGGGTAATGGTTGATGCTTACTTAAACCTGTTTAAAGAAGCGGATTTCATTGTGTCACCTAGTGCATCC
GGCTTTACTAAAACAATAGCTGCGGTGCAAAAGGGTGAGAGCTTTACTAACCTCGTGGATGACTTTTTACAACTAGCTAA
CTTTGCTGGTAATCCTTCAATTACGATTCCATGATTGGTCAAACAAAAGGACCAAACGATTGGTTTGAGTGTTAATGCTA
ACTGCTTTCACGACAAGCAATTATTACAAGTAGCTGCTTGATTGGAGGAATTATTCCAAATTGAACATGATGATTAA

Upstream 100 bases:

>100_bases
CAACGCCACTGCGTAAAGATAAGGTAATAGTGGAAAAGCATCCCGAAAAATTATTAGCCAACTGTAAGGAAATGAGTGTT
GGCTTTGTCAAAGTATAGTT

Downstream 100 bases:

>100_bases
CTTTGAAGCAATTATTGGGATTGAGGTACACGTGGTCTTAAACACTGCTACCAAAATGTTTTCCCCAGCGCCCAACCAAG
CGCAAAACGCCACACCAAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKSQILKLQQTLTKKPASINPLLQQIDGAINEHWSSNFLLKNTVEWAQAQAPKNRSKSPLNNIPFVLKDNIATKGIVTTG
GSRFLEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHPWNETLIPGGSSSGSAYAVARGIVPFSI
GTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTR
SLKESFNKQIKIGYLKPLEEWFDIELSKKWNSLKERITLEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIV
FGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSAS
GFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIPWLVKQKDQTIGLSVNANCFHDKQLLQVAAWLEELFQIEHDD

Sequences:

>Translated_478_residues
MKSQILKLQQTLTKKPASINPLLQQIDGAINEH*SSNFLLKNTVE*AQAQAPKNRSKSPLNNIPFVLKDNIATKGIVTTG
GSRFLEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHP*NETLIPGGSSSGSAYAVARGIVPFSI
GTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTR
SLKESFNKQIKIGYLKPLEEWFDIELSKK*NSLKERITLEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIV
FGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSAS
GFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIP*LVKQKDQTIGLSVNANCFHDKQLLQVAA*LEELFQIEHDD
>Mature_478_residues
MKSQILKLQQTLTKKPASINPLLQQIDGAINEH*SSNFLLKNTVE*AQAQAPKNRSKSPLNNIPFVLKDNIATKGIVTTG
GSRFLEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTGLHSGFGFVHHP*NETLIPGGSSSGSAYAVARGIVPFSI
GTDTGDSVRRPASICNIVGFKPTYGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTR
SLKESFNKQIKIGYLKPLEEWFDIELSKK*NSLKERITLEGCELIPFHFPLELLEVIDPVYKLISYSEAVSCYSNLTGIV
FGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKNNFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSAS
GFTKTIAAVQKGESFTNLVDDFLQLANFAGNPSITIP*LVKQKDQTIGLSVNANCFHDKQLLQVAA*LEELFQIEHDD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=457, Percent_Identity=32.6039387308534, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI195972892, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=456, Percent_Identity=32.4561403508772, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17556264, Length=163, Percent_Identity=30.6748466257669, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=441, Percent_Identity=32.4263038548753, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319685, Length=159, Percent_Identity=44.6540880503145, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648113, Length=465, Percent_Identity=33.1182795698925, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24644968, Length=444, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52005; Mature: 52005

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQILKLQQTLTKKPASINPLLQQIDGAINEHSSNFLLKNTVEAQAQAPKNRSKSPLNN
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHCCCCCCCCCCCCC
IPFVLKDNIATKGIVTTGGSRFLEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTG
CCEEEECCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
LHSGFGFVHHPNETLIPGGSSSGSAYAVARGIVPFSIGTDTGDSVRRPASICNIVGFKPT
CCCCCCEEECCCCCCCCCCCCCCCEEEHEECCEEEECCCCCCHHHHCCHHHHHHCCCCCC
YGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTRSLK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ESFNKQIKIGYLKPLEEWFDIELSKKNSLKERITLEGCELIPFHFPLELLEVIDPVYKLI
HHHCCEEEEEEHHHHHHHHCCEECCCCCHHHHHEECCCEEECCCCHHHHHHHHHHHHHHH
SYSEAVSCYSNLTGIVFGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKN
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCCC
NFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSASGFTKTIAAVQKGESFTNLVDDFLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LANFAGNPSITIPLVKQKDQTIGLSVNANCFHDKQLLQVAALEELFQIEHDD
HHHCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSQILKLQQTLTKKPASINPLLQQIDGAINEHSSNFLLKNTVEAQAQAPKNRSKSPLNN
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHHCCCCCCCCCCCCC
IPFVLKDNIATKGIVTTGGSRFLEDYIPPFSATVFELLNNSGALLVGKANLDEFGLGGTG
CCEEEECCCCCCCEEECCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
LHSGFGFVHHPNETLIPGGSSSGSAYAVARGIVPFSIGTDTGDSVRRPASICNIVGFKPT
CCCCCCEEECCCCCCCCCCCCCCCEEEHEECCEEEECCCCCCHHHHCCHHHHHHCCCCCC
YGLISRNGVYPYAPSLDHVGIFARYVYDVALVSDEIIKHDKADFSAQKSPDAGKFTRSLK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
ESFNKQIKIGYLKPLEEWFDIELSKKNSLKERITLEGCELIPFHFPLELLEVIDPVYKLI
HHHCCEEEEEEHHHHHHHHCCEECCCCCHHHHHEECCCEEECCCCHHHHHHHHHHHHHHH
SYSEAVSCYSNLTGIVFGQKLFEPNQASDFSKTITANRDRFFGEQLKRRFIIGAFGTDKN
HHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCCC
NFTKYFEKAQKIRRVMVDAYLNLFKEADFIVSPSASGFTKTIAAVQKGESFTNLVDDFLQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHH
LANFAGNPSITIPLVKQKDQTIGLSVNANCFHDKQLLQVAALEELFQIEHDD
HHHCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]