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The map label for this gene is potB [H]

Identifier: 13507795

GI number: 13507795

Start: 72088

End: 72948

Strand: Direct

Name: potB [H]

Synonym: MPN056

Alternate gene names: 13507795

Gene position: 72088-72948 (Clockwise)

Preceding gene: 13507794

Following gene: 13507796

Centisome position: 8.83

GC content: 38.68

Gene sequence:

>861_bases
ATGAAACTATCGAAAAAATACCTCTTAGCTGTACCATTCTTTGTTTTAATGGTCATCTTCTTTGTAGTACCAATGGCATG
GATTATTGTTAGTGGACTCCAAAATGAAAACGGTGCAAGTATAACGGAAAAGTACCAACCCTTAGTTGGTGGTTATTCTT
TCTTTCAGAGTTTTTGAACTAGTCTGTGAACGGCGACTGTGACAGTACTAGTAGCCTTACTGGTTGCCTTTCCCTTTTGT
TACTTTTTATCACAAAGTAAAAACAAGGTATTCCGCAGTTTTGTGATTGCCTTAGCTACTGCTCCCATATGGAGTAGTTT
TCTCATTAAGTTAATTGGGTTAAAAACACTGTTGGACTTAGTTTTGGGGTTAGCGTTGAACCGGGTGGGTGATAATAACC
TCACCTTTGGTAGTGGTTATACCTTGATTGGGATGATCTACCTGTTCACCCCCTTCATGTTTTTACCACTTTACAACAAC
TTTTGTATCTTACCGAAAAACTTAATCTTAGCTAGTCAAGATTTGGGTTACAACTGGATTACGAGTTTTATTAAGGTAGT
TATTCCCTTTTCTAAAACTGCCATTCTGTCAGGGATTGCGTTAACTTTCTTTCCGTCTTTAACCTCGGTAGCGATTGCCC
AGTTCTTGGACAACTCGAATCAAAATAACACCTTAGGTAACTATGTCTTTACTTTAGGTAACAATGGTTATGACAGTGCG
ATTGAGCGCGGCCGTGCTTCTGGTGCCATTATTATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGC
TCCAAGAATAGTGCGCTTAATCCAGACTAAGTGTTTGAAATACAGGAGGGTTAATGTTTAA

Upstream 100 bases:

>100_bases
GACACCATAGTAGATCTAGAGAGTGTCAACGACATTGAGGTTGACAAAACCTTTGGGGTCACCTTTGATCCGATTGATGT
TCACCTAATGGAGGTATAGG

Downstream 100 bases:

>100_bases
GATGAAAAGCTGTAAGTTATGACTACGGGGAAGTTTCTTTGTCATTGTCCTTGTTCTCATCTACCTCCCTTTAATAATAG
TTGTCTTAGTTAGTTTTAAC

Product: spermidine/putrescine transport system permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFWTSLWTATVTVLVALLVAFPFC
YFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNN
FCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSA
IERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV

Sequences:

>Translated_286_residues
MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSF*TSL*TATVTVLVALLVAFPFC
YFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNN
FCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSA
IERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV
>Mature_286_residues
MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSF*TSL*TATVTVLVALLVAFPFC
YFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVLGLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNN
FCILPKNLILASQDLGYNWITSFIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSA
IERGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV

Specific function: Required for the activity of the bacterial transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=227, Percent_Identity=27.3127753303965, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787080, Length=172, Percent_Identity=28.4883720930233, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31528; Mature: 31528

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTATVTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITS
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHEEECCCCCHHHHHH
FIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHH
RGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLSKKYLLAVPFFVLMVIFFVVPMAWIIVSGLQNENGASITEKYQPLVGGYSFFQSFTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTATVTVLVALLVAFPFCYFLSQSKNKVFRSFVIALATAPIWSSFLIKLIGLKTLLDLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLALNRVGDNNLTFGSGYTLIGMIYLFTPFMFLPLYNNFCILPKNLILASQDLGYNWITS
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCHHEEECCCCCHHHHHH
FIKVVIPFSKTAILSGIALTFFPSLTSVAIAQFLDNSNQNNTLGNYVFTLGNNGYDSAIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHH
RGRASGAIIIAALITFAFYFVVIFAPRIVRLIQTKCLKYRRVNV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]