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The map label for this gene is potI [H]

Identifier: 13507796

GI number: 13507796

Start: 72941

End: 73801

Strand: Direct

Name: potI [H]

Synonym: MPN057

Alternate gene names: 13507796

Gene position: 72941-73801 (Clockwise)

Preceding gene: 13507795

Following gene: 13507797

Centisome position: 8.93

GC content: 39.84

Gene sequence:

>861_bases
ATGTTTAAGATGAAAAGCTGTAAGTTATGACTACGGGGAAGTTTCTTTGTCATTGTCCTTGTTCTCATCTACCTCCCTTT
AATAATAGTTGTCTTAGTTAGTTTTAACGGTTCTTCGACTAGGGGTAACATTGTCTTAGACTTTGGTAATGTTTTAAACC
CTAACCCGGATGCCAAGTCAGCATATCTAAGATTAGGGGAAGCGGACTTTGCCATCCCTTTACTCAACTCTGTCATTATT
GGCTTAATTACGGTCATTGTCTCGATCCCGATTGCCATCATGACTGCCTTTGCTTTATTGCGATCCAGGCAATGGCTCAA
TAAAACAGTCTTTGGGATTGCTAACTTTTCTCTAGCAACTCCCGATATTATTACCGGGATATCGCTAGTGTTGTTATTTG
CCAACACCTGGTTGAGCTTTAACCAACAGTTAGGTTTCTTTACTATTATTAGTTCCCACATCTCCTTTTCGGTGCCTTAT
GCCTTGGTGTTAATTTACCCAAAGATGCAAAAGTTAAACCGTAACTTAATCCTAGCGAGTCAGGATTTGGGTTACTCACC
CATTGCTACCTTCTTTCACATTACCTTACCTTACTTACTACCGAGTATCTTGTCAGCCATTCTAGTAGTGTTTGCCACGA
GCTTTGATGACTATGTGATTACTTCCTTAGTTCAAGGTTCAGTTAAAACAGTCGCCAGTGAGCTCTATTCCTTTAGAAAG
GGGATTAAGGCCTGAGCTATTGCCTTTGGTACCATTTTAATCTTGGTCAGCATTCTAGCTGTCTTGCTAGTAACACTGCA
CAAGTACCTACGCTTTAAACATAAAGAAATGCTCCGTGTTAAACAATGAAAAAACAGTTAA

Upstream 100 bases:

>100_bases
ATTGCGGCTTTAATTACCTTTGCCTTTTACTTTGTGGTGATCTTTGCTCCAAGAATAGTGCGCTTAATCCAGACTAAGTG
TTTGAAATACAGGAGGGTTA

Downstream 100 bases:

>100_bases
AGTATTGGTTTAGTCTAATGGGACTAACCATGTCAGTTGGGTTAACTGCTTGTAGTTCCAACCAGTTTGTGTTTGCTAAC
TTTGAGTCGTATGTGTCGCC

Product: spermidine/putrescine transport system permease

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MFKMKSCKLWLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVII
GLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPY
ALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRK
GIKAWAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQWKNS

Sequences:

>Translated_286_residues
MFKMKSCKL*LRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVII
GLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPY
ALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRK
GIKA*AIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQ*KNS
>Mature_286_residues
MFKMKSCKL*LRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSAYLRLGEADFAIPLLNSVII
GLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATPDIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPY
ALVLIYPKMQKLNRNLILASQDLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRK
GIKA*AIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQ*KNS

Specific function: Required for the activity of the bacterial transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=174, Percent_Identity=32.183908045977, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787081, Length=295, Percent_Identity=29.8305084745763, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31283; Mature: 31283

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKMKSCKLLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHH
YLRLGEADFAIPLLNSVIIGLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
DLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKAAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKMKSCKLLRGSFFVIVLVLIYLPLIIVVLVSFNGSSTRGNIVLDFGNVLNPNPDAKSA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHH
YLRLGEADFAIPLLNSVIIGLITVIVSIPIAIMTAFALLRSRQWLNKTVFGIANFSLATP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
DIITGISLVLLFANTWLSFNQQLGFFTIISSHISFSVPYALVLIYPKMQKLNRNLILASQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEC
DLGYSPIATFFHITLPYLLPSILSAILVVFATSFDDYVITSLVQGSVKTVASELYSFRKG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKAAIAFGTILILVSILAVLLVTLHKYLRFKHKEMLRVKQKNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]