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The map label for this gene is dppA [H]

Identifier: 13488093

GI number: 13488093

Start: 128726

End: 130276

Strand: Reverse

Name: dppA [H]

Synonym: mll9149

Alternate gene names: 13488093

Gene position: 130276-128726 (Counterclockwise)

Preceding gene: 13488094

Following gene: 13488092

Centisome position: 37.02

GC content: 56.35

Gene sequence:

>1551_bases
ATGAATATGACTCGCCGAAATTACCTTAAGGTGATGAGTGCCATTCCACTGCTTGGCACAATCGCTCCGCGAATTGCCAA
CGCACAGCCCCGAACCGATTTGATCATTGCGGTTCAGGATAATCCGCCTCAACTCGATCCCCTCAGGCTCACTACGAACA
TCACATATCGTCTTGCGGCAAGCATCTACGACACGCTGATCCGTATCGATTACCTTAACGGCGAGCGCCTGGTGCCGGGC
CTTGCGGAGTCTTGGCACCAGGTCGATCCACGCACCTGGGATGTTGTCATCCGCAAAGGGGTCCACTTCCATGATGGATC
GGTGATGACCACCGAAGACGTTGCCTTTACGTTCGGACCGGAACGCATGTTGACGGAGGGCCTTCCGGGTCAAAGCATAT
CCCGGCAGTTTTTCAGCGTGCTGGAAAAGGTGGCCGCCGTCGACGAAAGCACTGTGCGTTTCGTAACCAAAGAGGCTGAT
CCCCTTTTCGAATTCAGGCTGTCCAACTGGACAAGTCAGATCATCAGCAAGGCTGCGTTCCAAAAGGCTGGAAACTGGGA
TAAATGGGCGCTATCACCGGTCGGCACCGGCCCTTATCGCGTCGCTGAAGTCAAGGCTGGCGATCGAATCCGACTGGTCG
CGCATGACCAATACTGGGGCGGCCAGCCTCCGTTCTCTTCGCTGACTTTCAAGGTCATGCCGGAAGCGGCAAGCCGCGTC
AACGCGCTCGCGGCGGGCGACGCCCACATCATCACGGAAGTTACGCCGGATCAGATCGCGACGATCAGGGGACACGGCAA
CCTTGATGTCGTCGGCGGGCCCATCAACAACATCCGTTGCGTCAACTACGGCACGTTCGGCGGGCCGCTCAAGGATGTTC
GCATCCGTCAGGCGCTCAATCTTTCTATCGACCGCGAATCCATTGCCAAACAATTGTTCTCAGGCATGGTTGATGTTCCC
AGAGGCTTTCAGTGGCAGGCCTACGGCGACATGTTCGTCGAGAATTTTCCGATGCCAGCATACGATCCCGATGCCGCGAA
GAAACTTCTTTCCGAGGCAGCCTATTCCGGTGCCCCGATCGAATATCGCATTCTGGCCAACTACTATACCGCAGAGCTCG
ACGTGGCGCAGACGCTCCAACAGATGTGGCAGGCCGTCGGCATCAATGTTCAGCTCAAGGTTTGCGAGAACTGGGGGCAA
ATGTTCGCTCAGCCAAATTACGCGATCTTCGACGGTTCGGCCGACATGATCTACCCGGATATATTGGGGTCGCTATGGAC
CAACTACGGGCCAGACGGCTTTATCCGCTTCGAAGCCAAGAGCTGGAGCAACGCGGAGTTCGACGAGATCGGCAAAAAAC
TGCAGACGCTGACTGACCGTACTGAACGCCGCAAGCTTCACGAACGCGTTCTGAAAATCTTCAATGAGATCGATCCGCCT
GGCACTGTGCTCTACAATACGCCCATGCTTTACGGCAAGCGGGGAGACATTGCCTGGCTGCCGCATCGCACCCCGATGAT
GGACTTCGGTCCATTCAACATGCCCGCATAG

Upstream 100 bases:

>100_bases
TTCGACTGTCATTCTATCATGCGAACGCTCTTATGTAGACATGTCTAACCAAGTTGCGAAGATCAGCTTCTGCTAACTTG
ACAATGGAGGGTAGCGCACC

Downstream 100 bases:

>100_bases
TCTTGTGCTTACCGGGCATCATGCGCCCGTCAGGGCGTATGATGCCCATGCATCATCCAATTCATCCACCGGGTTCCGAG
GTCGATTGAGGCTGTCGGTG

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNMTRRNYLKVMSAIPLLGTIAPRIANAQPRTDLIIAVQDNPPQLDPLRLTTNITYRLAASIYDTLIRIDYLNGERLVPG
LAESWHQVDPRTWDVVIRKGVHFHDGSVMTTEDVAFTFGPERMLTEGLPGQSISRQFFSVLEKVAAVDESTVRFVTKEAD
PLFEFRLSNWTSQIISKAAFQKAGNWDKWALSPVGTGPYRVAEVKAGDRIRLVAHDQYWGGQPPFSSLTFKVMPEAASRV
NALAAGDAHIITEVTPDQIATIRGHGNLDVVGGPINNIRCVNYGTFGGPLKDVRIRQALNLSIDRESIAKQLFSGMVDVP
RGFQWQAYGDMFVENFPMPAYDPDAAKKLLSEAAYSGAPIEYRILANYYTAELDVAQTLQQMWQAVGINVQLKVCENWGQ
MFAQPNYAIFDGSADMIYPDILGSLWTNYGPDGFIRFEAKSWSNAEFDEIGKKLQTLTDRTERRKLHERVLKIFNEIDPP
GTVLYNTPMLYGKRGDIAWLPHRTPMMDFGPFNMPA

Sequences:

>Translated_516_residues
MNMTRRNYLKVMSAIPLLGTIAPRIANAQPRTDLIIAVQDNPPQLDPLRLTTNITYRLAASIYDTLIRIDYLNGERLVPG
LAESWHQVDPRTWDVVIRKGVHFHDGSVMTTEDVAFTFGPERMLTEGLPGQSISRQFFSVLEKVAAVDESTVRFVTKEAD
PLFEFRLSNWTSQIISKAAFQKAGNWDKWALSPVGTGPYRVAEVKAGDRIRLVAHDQYWGGQPPFSSLTFKVMPEAASRV
NALAAGDAHIITEVTPDQIATIRGHGNLDVVGGPINNIRCVNYGTFGGPLKDVRIRQALNLSIDRESIAKQLFSGMVDVP
RGFQWQAYGDMFVENFPMPAYDPDAAKKLLSEAAYSGAPIEYRILANYYTAELDVAQTLQQMWQAVGINVQLKVCENWGQ
MFAQPNYAIFDGSADMIYPDILGSLWTNYGPDGFIRFEAKSWSNAEFDEIGKKLQTLTDRTERRKLHERVLKIFNEIDPP
GTVLYNTPMLYGKRGDIAWLPHRTPMMDFGPFNMPA
>Mature_516_residues
MNMTRRNYLKVMSAIPLLGTIAPRIANAQPRTDLIIAVQDNPPQLDPLRLTTNITYRLAASIYDTLIRIDYLNGERLVPG
LAESWHQVDPRTWDVVIRKGVHFHDGSVMTTEDVAFTFGPERMLTEGLPGQSISRQFFSVLEKVAAVDESTVRFVTKEAD
PLFEFRLSNWTSQIISKAAFQKAGNWDKWALSPVGTGPYRVAEVKAGDRIRLVAHDQYWGGQPPFSSLTFKVMPEAASRV
NALAAGDAHIITEVTPDQIATIRGHGNLDVVGGPINNIRCVNYGTFGGPLKDVRIRQALNLSIDRESIAKQLFSGMVDVP
RGFQWQAYGDMFVENFPMPAYDPDAAKKLLSEAAYSGAPIEYRILANYYTAELDVAQTLQQMWQAVGINVQLKVCENWGQ
MFAQPNYAIFDGSADMIYPDILGSLWTNYGPDGFIRFEAKSWSNAEFDEIGKKLQTLTDRTERRKLHERVLKIFNEIDPP
GTVLYNTPMLYGKRGDIAWLPHRTPMMDFGPFNMPA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=509, Percent_Identity=24.950884086444, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787052, Length=466, Percent_Identity=24.8927038626609, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787551, Length=281, Percent_Identity=27.0462633451957, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787762, Length=470, Percent_Identity=24.2553191489362, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789887, Length=472, Percent_Identity=23.5169491525424, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789397, Length=542, Percent_Identity=22.3247232472325, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082063, Length=270, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57932; Mature: 57932

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTRRNYLKVMSAIPLLGTIAPRIANAQPRTDLIIAVQDNPPQLDPLRLTTNITYRLAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHH
SIYDTLIRIDYLNGERLVPGLAESWHQVDPRTWDVVIRKGVHFHDGSVMTTEDVAFTFGP
HHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHCCCEEECCCEEEECCEEEEECC
ERMLTEGLPGQSISRQFFSVLEKVAAVDESTVRFVTKEADPLFEFRLSNWTSQIISKAAF
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHH
QKAGNWDKWALSPVGTGPYRVAEVKAGDRIRLVAHDQYWGGQPPFSSLTFKVMPEAASRV
HHCCCCCCEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHH
NALAAGDAHIITEVTPDQIATIRGHGNLDVVGGPINNIRCVNYGTFGGPLKDVRIRQALN
HHHCCCCCEEEEECCCCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHC
LSIDRESIAKQLFSGMVDVPRGFQWQAYGDMFVENFPMPAYDPDAAKKLLSEAAYSGAPI
CCCCHHHHHHHHHHCHHCCCCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCE
EYRILANYYTAELDVAQTLQQMWQAVGINVQLKVCENWGQMFAQPNYAIFDGSADMIYPD
EEEEEECEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCEEEECCCCCEECHH
ILGSLWTNYGPDGFIRFEAKSWSNAEFDEIGKKLQTLTDRTERRKLHERVLKIFNEIDPP
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTVLYNTPMLYGKRGDIAWLPHRTPMMDFGPFNMPA
CEEEEECCEEECCCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNMTRRNYLKVMSAIPLLGTIAPRIANAQPRTDLIIAVQDNPPQLDPLRLTTNITYRLAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEECHHHHHHH
SIYDTLIRIDYLNGERLVPGLAESWHQVDPRTWDVVIRKGVHFHDGSVMTTEDVAFTFGP
HHHHHHHEEEECCCCCCCCHHHHHHHHCCCCCEEHHHHCCCEEECCCEEEECCEEEEECC
ERMLTEGLPGQSISRQFFSVLEKVAAVDESTVRFVTKEADPLFEFRLSNWTSQIISKAAF
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHH
QKAGNWDKWALSPVGTGPYRVAEVKAGDRIRLVAHDQYWGGQPPFSSLTFKVMPEAASRV
HHCCCCCCEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHH
NALAAGDAHIITEVTPDQIATIRGHGNLDVVGGPINNIRCVNYGTFGGPLKDVRIRQALN
HHHCCCCCEEEEECCCCCEEEEECCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHC
LSIDRESIAKQLFSGMVDVPRGFQWQAYGDMFVENFPMPAYDPDAAKKLLSEAAYSGAPI
CCCCHHHHHHHHHHCHHCCCCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCE
EYRILANYYTAELDVAQTLQQMWQAVGINVQLKVCENWGQMFAQPNYAIFDGSADMIYPD
EEEEEECEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCEEEECCCCCEECHH
ILGSLWTNYGPDGFIRFEAKSWSNAEFDEIGKKLQTLTDRTERRKLHERVLKIFNEIDPP
HHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GTVLYNTPMLYGKRGDIAWLPHRTPMMDFGPFNMPA
CEEEEECCEEECCCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]