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The map label for this gene is bioB [H]

Identifier: 13488069

GI number: 13488069

Start: 79223

End: 80161

Strand: Reverse

Name: bioB [H]

Synonym: mll9100

Alternate gene names: 13488069

Gene position: 80161-79223 (Counterclockwise)

Preceding gene: 13488070

Following gene: 13488068

Centisome position: 22.78

GC content: 61.24

Gene sequence:

>939_bases
ATGTGGACCCTGGATAAGGCTCAGGCGCTTCACGACGCTCCCTTCAACAATCTGCTGTTCCTGGCGCACACCGTGCACCG
CCAGAATTTCGATCCCAACAAGGTCCAGCTCAGCCGGCTCTTGAGCATCAAGACAGGCGGATGTCCGGAGGATTGCGGCT
ATTGCAGCCAGTCGGCGCATCACGAAACCGGCCTGAAGGCGTCGAAGCTGATGGAGGTCAGGCGCGTCATCGCCGAGGCA
ACCAAGGCGCGCGATGCCGGCGCCACCCGCTATTGCATGGGTGCTGCCTGGCGAAATCCCAAGCCGCGCGACATGGATGC
GGTCGTGGCGATGGTCGAAGGCGTCAAGGCGCTGGGCATGGAAACCTGCATGACACTCGGCATGCTCGATCTCGGCCAGG
CGCAGCAACTGAAACAGGCTGGTCTCGATTATTACAACCACAACATCGATACGTCGGAACGCTACTATAGCGAGATCATC
AGCACGCGCAGCTTTGCCGACCGGCTCGATACGCTCGAACAGGTTCGCCAATCCGGCATCAAGGTCTGTTGCGGCGGCAT
TGTCGGCATGGGCGAAGAGCCGGTGGACCGGATCGACATGCTGGTGACACTGGCCAATCTGCCGGAGCATCCGCAAAGCG
TTCCGATCAACATGCTGATCCCGATCGAAGGCACCCCGCTTGCCGAAACCAGGCCGATCGAGCCGATCGAATTCGTGCGC
GTGATCGCGCTGGCACGCATCATGATGCCGAAATCGCATGTGCGCCTTTCGGCCGGCCGCACCGCCATGACCGACGAGAT
GCAGGCGCTGTGCTTCTTTGCCGGCGCCAACTCGATCTTCGTCGGCGACACGCTGCTGACGGCGGACAATCCCGGCGAGG
ACGAAGATACCGCGCTGTTCCGGCGTCTCGGCATCCAGCGCATGGAACGTGAAACATAA

Upstream 100 bases:

>100_bases
GAAAAGAAGCCGTAACGCCGGAACCCCAGGGAACAGGACAGGGATGCACGTATCGAATTCGACGAAGACAATGCCACAGG
CGCAATGCGACGATGGTGTG

Downstream 100 bases:

>100_bases
TGAGCGCAAGGCCCCTCGCCCGCTATGACGCGACCTTGCGCGGACTGGCGCGCAACGACCGGCTGCGGACGCTGTCGCCG
CGCGCCGGCCTCGACTTCTC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MWTLDKAQALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHETGLKASKLMEVRRVIAEA
TKARDAGATRYCMGAAWRNPKPRDMDAVVAMVEGVKALGMETCMTLGMLDLGQAQQLKQAGLDYYNHNIDTSERYYSEII
STRSFADRLDTLEQVRQSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPQSVPINMLIPIEGTPLAETRPIEPIEFVR
VIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDEDTALFRRLGIQRMERET

Sequences:

>Translated_312_residues
MWTLDKAQALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHETGLKASKLMEVRRVIAEA
TKARDAGATRYCMGAAWRNPKPRDMDAVVAMVEGVKALGMETCMTLGMLDLGQAQQLKQAGLDYYNHNIDTSERYYSEII
STRSFADRLDTLEQVRQSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPQSVPINMLIPIEGTPLAETRPIEPIEFVR
VIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDEDTALFRRLGIQRMERET
>Mature_312_residues
MWTLDKAQALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAHHETGLKASKLMEVRRVIAEA
TKARDAGATRYCMGAAWRNPKPRDMDAVVAMVEGVKALGMETCMTLGMLDLGQAQQLKQAGLDYYNHNIDTSERYYSEII
STRSFADRLDTLEQVRQSGIKVCCGGIVGMGEEPVDRIDMLVTLANLPEHPQSVPINMLIPIEGTPLAETRPIEPIEFVR
VIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDEDTALFRRLGIQRMERET

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=304, Percent_Identity=59.8684210526316, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=48.7341772151899, Blast_Score=321, Evalue=9e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 34569; Mature: 34569

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTLDKAQALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAH
CCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCC
HETGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKPRDMDAVVAMVEGVKALGM
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETCMTLGMLDLGQAQQLKQAGLDYYNHNIDTSERYYSEIISTRSFADRLDTLEQVRQSGI
HHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KVCCGGIVGMGEEPVDRIDMLVTLANLPEHPQSVPINMLIPIEGTPLAETRPIEPIEFVR
HEEECHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
VIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDEDTALF
HHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCHHHHHH
RRLGIQRMERET
HHHHHHHHHCCC
>Mature Secondary Structure
MWTLDKAQALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKTGGCPEDCGYCSQSAH
CCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCC
HETGLKASKLMEVRRVIAEATKARDAGATRYCMGAAWRNPKPRDMDAVVAMVEGVKALGM
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
ETCMTLGMLDLGQAQQLKQAGLDYYNHNIDTSERYYSEIISTRSFADRLDTLEQVRQSGI
HHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
KVCCGGIVGMGEEPVDRIDMLVTLANLPEHPQSVPINMLIPIEGTPLAETRPIEPIEFVR
HEEECHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
VIALARIMMPKSHVRLSAGRTAMTDEMQALCFFAGANSIFVGDTLLTADNPGEDEDTALF
HHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCHHHHHH
RRLGIQRMERET
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA