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The map label for this gene is rfbB [H]

Identifier: 13476273

GI number: 13476273

Start: 6233506

End: 6234576

Strand: Direct

Name: rfbB [H]

Synonym: mlr7552

Alternate gene names: 13476273

Gene position: 6233506-6234576 (Clockwise)

Preceding gene: 13476272

Following gene: 13476274

Centisome position: 88.59

GC content: 63.4

Gene sequence:

>1071_bases
ATGAATTTTCTGGTGACAGGCGGGGCTGGCTTCATCGGTTCGGCGGTGTGTCGGCATCTGTGCGCCAATCCGGCCTATCG
GGTGACCAATCTCGACAAGCTCACCTATGCCGGCAACCTGGCCTCGCTGCGGCAGATCGAGAACGCGCATAATTATCGTT
TCGCCCATGCCGATATCTGCGACGAGAGGGCGGTGCTCGACATAATGCGCCGTGACGATATCGACATCGTCATGAACCTT
GCCGCCGAAAGCCATGTCGACCGCTCGATCGATGGGCCCGGCGCCTTCATCGAGACCAACATCGTCGGCACCTACCGCAT
CCTCAATGCGGCGCTCGAATACTGGCGCGGCTTGCCCGACGACAGGAAAAGCCGCTTCCGCTTCCATCACGTGTCGACCG
ATGAAGTGTTCGGCGACCTGCCTTTCGACGGTGGCATGTTCGTCGAGGAGACGCCCTACGCGCCGTCCTCGCCCTATTCG
GCCTCCAAGGCGGCCTCGGACCATCTGGTGCGGGCCTGGCACGAGACCTACGGCCTGCCGGTCGTGCTCTCCAACTGCTC
GAACAATTACGGCCCCTATCATTTTCCCGAAAAACTGATCCCGCTCGTCATTCTCAACGCGCTCGACGAAAAACCGCTGC
CAGTCTATGGCGCCGGCGCCAATGTGCGTGACTGGCTGTTCGTCGAGGATCATGCCCGAGCCCTGGAGCTGGTCGCCACC
AAGGGCACGCCGGGCGAGAGCTATAATGTCGGCGGCAATTCGGAACGCACGAACCTCGCCGTCGTCGAGACGATCTGCGA
CCTGCTCGACATCAGGCGGCCCCGGGCCGGCGGCAGGTGTTATCGCGACCTGATCTCCTTCGTCACCGACCGCCCGGGCC
ACGACCGCCGCTACGCCATCGATGCCTCCAAGATCGGCCGCGAGCTCGGCTGGGCGCCGAGCGAGAATTTCGACAGCGGC
CTGGCCAGAACCGTGGACTGGTTCCTCGACAACAAATGGTGGTGGGGGCCGATCCGCGAGCAGCGGTACGCCGGTGAAAG
GCTGGGCGAGGCCCGCAAGGTGGGCGCGTGA

Upstream 100 bases:

>100_bases
CCTTCAGTGGCTGGCGGATTCCAGCTTTCGGACAAGGACCTCAAGGCGCCGGTGCTCGCCGAGGCCGAAGTGTTTGCCTG
ATGGGCATGAGGATAGCGGC

Downstream 100 bases:

>100_bases
GGCTCGCTGTCACCGGACGCGACGGCCAGGTTGTGTCGAGCCTTCTGGAGGCAGGCCAGTTTGCCGGTGTAGACGTCATC
GCCATTGGCCGTCCGCAGCT

Product: dTDP-D-glucose-4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNFLVTGGAGFIGSAVCRHLCANPAYRVTNLDKLTYAGNLASLRQIENAHNYRFAHADICDERAVLDIMRRDDIDIVMNL
AAESHVDRSIDGPGAFIETNIVGTYRILNAALEYWRGLPDDRKSRFRFHHVSTDEVFGDLPFDGGMFVEETPYAPSSPYS
ASKAASDHLVRAWHETYGLPVVLSNCSNNYGPYHFPEKLIPLVILNALDEKPLPVYGAGANVRDWLFVEDHARALELVAT
KGTPGESYNVGGNSERTNLAVVETICDLLDIRRPRAGGRCYRDLISFVTDRPGHDRRYAIDASKIGRELGWAPSENFDSG
LARTVDWFLDNKWWWGPIREQRYAGERLGEARKVGA

Sequences:

>Translated_356_residues
MNFLVTGGAGFIGSAVCRHLCANPAYRVTNLDKLTYAGNLASLRQIENAHNYRFAHADICDERAVLDIMRRDDIDIVMNL
AAESHVDRSIDGPGAFIETNIVGTYRILNAALEYWRGLPDDRKSRFRFHHVSTDEVFGDLPFDGGMFVEETPYAPSSPYS
ASKAASDHLVRAWHETYGLPVVLSNCSNNYGPYHFPEKLIPLVILNALDEKPLPVYGAGANVRDWLFVEDHARALELVAT
KGTPGESYNVGGNSERTNLAVVETICDLLDIRRPRAGGRCYRDLISFVTDRPGHDRRYAIDASKIGRELGWAPSENFDSG
LARTVDWFLDNKWWWGPIREQRYAGERLGEARKVGA
>Mature_356_residues
MNFLVTGGAGFIGSAVCRHLCANPAYRVTNLDKLTYAGNLASLRQIENAHNYRFAHADICDERAVLDIMRRDDIDIVMNL
AAESHVDRSIDGPGAFIETNIVGTYRILNAALEYWRGLPDDRKSRFRFHHVSTDEVFGDLPFDGGMFVEETPYAPSSPYS
ASKAASDHLVRAWHETYGLPVVLSNCSNNYGPYHFPEKLIPLVILNALDEKPLPVYGAGANVRDWLFVEDHARALELVAT
KGTPGESYNVGGNSERTNLAVVETICDLLDIRRPRAGGRCYRDLISFVTDRPGHDRRYAIDASKIGRELGWAPSENFDSG
LARTVDWFLDNKWWWGPIREQRYAGERLGEARKVGA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=36.9369369369369, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=23.5649546827795, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=62.5714285714286, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1788353, Length=363, Percent_Identity=60.0550964187328, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1786974, Length=371, Percent_Identity=28.0323450134771, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788366, Length=343, Percent_Identity=25.0728862973761, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=339, Percent_Identity=34.8082595870207, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=31.9148936170213, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=26.3013698630137, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=367, Percent_Identity=26.158038147139, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=353, Percent_Identity=24.3626062322946, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=379, Percent_Identity=26.1213720316623, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19923002, Length=375, Percent_Identity=24.8, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39848; Mature: 39848

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLVTGGAGFIGSAVCRHLCANPAYRVTNLDKLTYAGNLASLRQIENAHNYRFAHADIC
CCEEEECCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHH
DERAVLDIMRRDDIDIVMNLAAESHVDRSIDGPGAFIETNIVGTYRILNAALEYWRGLPD
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCC
DRKSRFRFHHVSTDEVFGDLPFDGGMFVEETPYAPSSPYSASKAASDHLVRAWHETYGLP
CHHHCEEEEECCHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VVLSNCSNNYGPYHFPEKLIPLVILNALDEKPLPVYGAGANVRDWLFVEDHARALELVAT
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCHHHEEEEEEE
KGTPGESYNVGGNSERTNLAVVETICDLLDIRRPRAGGRCYRDLISFVTDRPGHDRRYAI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
DASKIGRELGWAPSENFDSGLARTVDWFLDNKWWWGPIREQRYAGERLGEARKVGA
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MNFLVTGGAGFIGSAVCRHLCANPAYRVTNLDKLTYAGNLASLRQIENAHNYRFAHADIC
CCEEEECCCHHHHHHHHHHHHCCCCEEECCCHHHHCCCCHHHHHHHHHCCCEEEEEHHHH
DERAVLDIMRRDDIDIVMNLAAESHVDRSIDGPGAFIETNIVGTYRILNAALEYWRGLPD
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCC
DRKSRFRFHHVSTDEVFGDLPFDGGMFVEETPYAPSSPYSASKAASDHLVRAWHETYGLP
CHHHCEEEEECCHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VVLSNCSNNYGPYHFPEKLIPLVILNALDEKPLPVYGAGANVRDWLFVEDHARALELVAT
EEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCHHHEEEEEEE
KGTPGESYNVGGNSERTNLAVVETICDLLDIRRPRAGGRCYRDLISFVTDRPGHDRRYAI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
DASKIGRELGWAPSENFDSGLARTVDWFLDNKWWWGPIREQRYAGERLGEARKVGA
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]