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The map label for this gene is clcB [H]

Identifier: 13476121

GI number: 13476121

Start: 6083376

End: 6084560

Strand: Reverse

Name: clcB [H]

Synonym: mll7352

Alternate gene names: 13476121

Gene position: 6084560-6083376 (Counterclockwise)

Preceding gene: 13476122

Following gene: 13476120

Centisome position: 86.48

GC content: 64.64

Gene sequence:

>1185_bases
ATGAAGATCCGCAGCATCACGGCCACGCCGATCAATCTTCGTCTCGAAGCGCCCTATGGCTGGGTGTTCGGCGAGCTCGA
CGGGTTTTCGCAGACCATTGTCGAGGTCGAGACCGAGGATGGGCTGATCGGCCTCGGCGAAGCGCCGACGCCGGCGGCGG
CCGCGATCATCAATGATGTTCTGGCCGAGCGCCTGGTCGGGCGCGATGCCTTCGACATCGCCGGCGCCGAGCATGTCTGC
CTGCCCTACTGGACCGGTGTGCAGTCGATCAACGACCGCACCCGCATCATGGCTTTCGGTGCGATCGAGATGGCGTTGTG
GGACCTGCGCGGCAAGGCGTGGAACCAGCCGCTCTACCAGCTGCTCGGCGGCGCCGTGCGCAAGGACATCCCGTTCACCG
ACTATTTTTCGCTGCGCGGCGACGGGCCGAAGGTGAAAGGCGAGACGACGCCGGAAGAAGTCGCCGACTATTGCGTCGAG
CTGCACGAGACGCATGGAACGACCTTCTTCGAAGGCAAGTTCTCGACCGAGGACCCGAAAGTCTCGCTCAGAATGGTCGA
GCTGATCCGCGCAAAGCTCGGCGACGAGGCGATGATCCGCATCGATTCCAACCAGGCCTATTCGCTGGCGACGGCGCGAC
GGCTGGCGCGGCCGCTCGAAGAGCTCGGCGTGCGCAACTGGGAGGATCCGGTGGCGACCATCGAGGAGATGCGCCAGTTG
CGGCAGCATTGCTCGATCCCGTTCTCGACCCACAATATCGACATTGCGCGCGCCATGGACCTCAAGGTCCCCGATGCCTT
CGTCGGCAATCCGACCGCGCATGGCGGCATCGGCCGCCTGCTGCGTTTCGTCGGCGCCTGCGAGCACGCCGGCGTCGATT
TCTGGTGCTACAGCGGCGACAGCGGCATCGGCAGCGCGGCCTATCTGCATCTGTGCGCGGCGCTCGGCTGGATCCGGGAG
CCGAACCAGTCGCTGTTTCGCATGCAGCCCATGGACATCACCGAGGAAGGGCCGTTTTCGCCGAGGAACAATGTCGTGCG
CGTGCCGGAAGGTCCCGGTCTCGGCGTCACCTTGTCGCGGAAAAACCTCGCCGCCTGCCATCGCGATTTCGCCGAGAACG
GCCCGTGCAACAAATATCATGACCCGGCAAAGCCCGGAACATATCGCCGCCTGCCGCTGAACTAG

Upstream 100 bases:

>100_bases
CCCGTCACCGGCAGCCATATGGGCATTCCAGACGGACCCGGCCTCGGCATCGACGTGGATCCGAGCCTGCTCGGCAAGCC
CTTCTTCGAGATCGGCTGAG

Downstream 100 bases:

>100_bases
GAGAACGAGAAGGAAAGGAGACTAGCCAACACAAGTGTCCGCCGCAGCGATCTGGATCGCCATGGCAGGGCCTGACGGGT
GAAAGCATGGCCTCAAGAAA

Product: chloromuconate cycloisomerase

Products: NA

Alternate protein names: Muconate cycloisomerase II [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKIRSITATPINLRLEAPYGWVFGELDGFSQTIVEVETEDGLIGLGEAPTPAAAAIINDVLAERLVGRDAFDIAGAEHVC
LPYWTGVQSINDRTRIMAFGAIEMALWDLRGKAWNQPLYQLLGGAVRKDIPFTDYFSLRGDGPKVKGETTPEEVADYCVE
LHETHGTTFFEGKFSTEDPKVSLRMVELIRAKLGDEAMIRIDSNQAYSLATARRLARPLEELGVRNWEDPVATIEEMRQL
RQHCSIPFSTHNIDIARAMDLKVPDAFVGNPTAHGGIGRLLRFVGACEHAGVDFWCYSGDSGIGSAAYLHLCAALGWIRE
PNQSLFRMQPMDITEEGPFSPRNNVVRVPEGPGLGVTLSRKNLAACHRDFAENGPCNKYHDPAKPGTYRRLPLN

Sequences:

>Translated_394_residues
MKIRSITATPINLRLEAPYGWVFGELDGFSQTIVEVETEDGLIGLGEAPTPAAAAIINDVLAERLVGRDAFDIAGAEHVC
LPYWTGVQSINDRTRIMAFGAIEMALWDLRGKAWNQPLYQLLGGAVRKDIPFTDYFSLRGDGPKVKGETTPEEVADYCVE
LHETHGTTFFEGKFSTEDPKVSLRMVELIRAKLGDEAMIRIDSNQAYSLATARRLARPLEELGVRNWEDPVATIEEMRQL
RQHCSIPFSTHNIDIARAMDLKVPDAFVGNPTAHGGIGRLLRFVGACEHAGVDFWCYSGDSGIGSAAYLHLCAALGWIRE
PNQSLFRMQPMDITEEGPFSPRNNVVRVPEGPGLGVTLSRKNLAACHRDFAENGPCNKYHDPAKPGTYRRLPLN
>Mature_394_residues
MKIRSITATPINLRLEAPYGWVFGELDGFSQTIVEVETEDGLIGLGEAPTPAAAAIINDVLAERLVGRDAFDIAGAEHVC
LPYWTGVQSINDRTRIMAFGAIEMALWDLRGKAWNQPLYQLLGGAVRKDIPFTDYFSLRGDGPKVKGETTPEEVADYCVE
LHETHGTTFFEGKFSTEDPKVSLRMVELIRAKLGDEAMIRIDSNQAYSLATARRLARPLEELGVRNWEDPVATIEEMRQL
RQHCSIPFSTHNIDIARAMDLKVPDAFVGNPTAHGGIGRLLRFVGACEHAGVDFWCYSGDSGIGSAAYLHLCAALGWIRE
PNQSLFRMQPMDITEEGPFSPRNNVVRVPEGPGLGVTLSRKNLAACHRDFAENGPCNKYHDPAKPGTYRRLPLN

Specific function: Highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=295, Percent_Identity=26.7796610169492, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.7 [H]

Molecular weight: Translated: 43552; Mature: 43552

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSITATPINLRLEAPYGWVFGELDGFSQTIVEVETEDGLIGLGEAPTPAAAAIINDV
CCCCEEECCCEEEEEECCCCEEEECCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHH
LAERLVGRDAFDIAGAEHVCLPYWTGVQSINDRTRIMAFGAIEMALWDLRGKAWNQPLYQ
HHHHHHCCCCHHHCCCCCEEEEECCCCHHCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHH
LLGGAVRKDIPFTDYFSLRGDGPKVKGETTPEEVADYCVELHETHGTTFFEGKFSTEDPK
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
VSLRMVELIRAKLGDEAMIRIDSNQAYSLATARRLARPLEELGVRNWEDPVATIEEMRQL
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RQHCSIPFSTHNIDIARAMDLKVPDAFVGNPTAHGGIGRLLRFVGACEHAGVDFWCYSGD
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
SGIGSAAYLHLCAALGWIREPNQSLFRMQPMDITEEGPFSPRNNVVRVPEGPGLGVTLSR
CCCCHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCEEEECC
KNLAACHRDFAENGPCNKYHDPAKPGTYRRLPLN
HHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MKIRSITATPINLRLEAPYGWVFGELDGFSQTIVEVETEDGLIGLGEAPTPAAAAIINDV
CCCCEEECCCEEEEEECCCCEEEECCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHH
LAERLVGRDAFDIAGAEHVCLPYWTGVQSINDRTRIMAFGAIEMALWDLRGKAWNQPLYQ
HHHHHHCCCCHHHCCCCCEEEEECCCCHHCCCCEEEEEEHHHHHHHHHHCCCCCCCHHHH
LLGGAVRKDIPFTDYFSLRGDGPKVKGETTPEEVADYCVELHETHGTTFFEGKFSTEDPK
HHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
VSLRMVELIRAKLGDEAMIRIDSNQAYSLATARRLARPLEELGVRNWEDPVATIEEMRQL
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
RQHCSIPFSTHNIDIARAMDLKVPDAFVGNPTAHGGIGRLLRFVGACEHAGVDFWCYSGD
HHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
SGIGSAAYLHLCAALGWIREPNQSLFRMQPMDITEEGPFSPRNNVVRVPEGPGLGVTLSR
CCCCHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCCCCEEECCCCCCCEEEECC
KNLAACHRDFAENGPCNKYHDPAKPGTYRRLPLN
HHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3299368; 3609743; 2583528 [H]