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The map label for this gene is ykfB [H]

Identifier: 13476122

GI number: 13476122

Start: 6084562

End: 6085665

Strand: Reverse

Name: ykfB [H]

Synonym: mll7353

Alternate gene names: 13476122

Gene position: 6085665-6084562 (Counterclockwise)

Preceding gene: 13476123

Following gene: 13476121

Centisome position: 86.49

GC content: 66.12

Gene sequence:

>1104_bases
GTGCAGATCAAATCCATCAAAGCCTACCATGTCGTGCAGCCCTTCGTGGACGGCCCCTACCGCATGTCCAAGGGACGCCA
GGCCGACGCCTTCGACGCGGTGATCGTTGCCATCACCGCCGACAGCGGCCTGACCGGCTGGGGCGAGATGGCGCCGCTCG
GCAATTTCTACTCGGCCGCGTTTCCGGCCGGCGCCCGTGCGGGCGTGCCGGAAATCGCGCCGCATCTCATCGGCCACGAT
CCGCGCGGGCTGGCCAGCATCGGCCGGCTGATGGACACGGTGTTCAAGGGCCATCCCTACATCAAGTCGGCGCTCGACAT
GGCCTGCTGGGATCTCGCCGCGCGCGCCGCCGGCGTGCCCCTCGTCACCATGCTCGGCGGCCGCGAGAGCGATATGGCGG
AGACCTACCGGGTCGTCACCCACGGCACGCTCGATCAGATGGCGGCACTGGCAAAAAGGATCATTGCGGAGGGCTGTCGC
CGTCTGCAGGTCAAGGTCGGCGGCAATGTGCACGATGACATCGAGCGGGTAACGACAGTCGCCTCGGCCGTGCCGAAAGG
CACCGTGATTTTCTGCGACGCCAATGCCGGCTGGACACCCTACCAAGCGCGGCAGTTCGCCGATGCCACGCGCGGCATCG
ACTACACGTTCGAGCAGCCCTGCACCACGATCGAGGAGAACATGTCGGTGCGCCGCATGCTCGACAAGCCGATGGTGCTC
GACGAATCCGTCACCTCGCTGGAAGAGATGCTGGAGATCCACCGCCTGGGCGCGGCCGACGGGTTGACGCTGAAGATCTC
GCGCCTGGGCGGCGTGACCAGGACCCGGCAGATCCGCGACGTTGCCGTCGATCTTGGCTTCATGATCACCGTCGAGGACA
CGGGCGGCGCCGAGATCGACACGGCGGCGATGGCACATCTATCGCTGTCGACGCCACAAGAGCGGCGATTGCATGCCATT
GCCTTCCATGAATGGGTGACGGTGCGCACGGCCTTCAACGCCCCGCCCGTCACCGGCAGCCATATGGGCATTCCAGACGG
ACCCGGCCTCGGCATCGACGTGGATCCGAGCCTGCTCGGCAAGCCCTTCTTCGAGATCGGCTGA

Upstream 100 bases:

>100_bases
CGACTTCGAGCAGTTGCTCTCCTATGCGCGCGGCCTCGAACAGCGCCCGTCCAAGGGCGCCTGACCAATTCCCCAGATAT
GTAGCCAGCGGACAGACAGA

Downstream 100 bases:

>100_bases
GATGAAGATCCGCAGCATCACGGCCACGCCGATCAATCTTCGTCTCGAAGCGCCCTATGGCTGGGTGTTCGGCGAGCTCG
ACGGGTTTTCGCAGACCATT

Product: muconate lactonizing enzyme

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQIKSIKAYHVVQPFVDGPYRMSKGRQADAFDAVIVAITADSGLTGWGEMAPLGNFYSAAFPAGARAGVPEIAPHLIGHD
PRGLASIGRLMDTVFKGHPYIKSALDMACWDLAARAAGVPLVTMLGGRESDMAETYRVVTHGTLDQMAALAKRIIAEGCR
RLQVKVGGNVHDDIERVTTVASAVPKGTVIFCDANAGWTPYQARQFADATRGIDYTFEQPCTTIEENMSVRRMLDKPMVL
DESVTSLEEMLEIHRLGAADGLTLKISRLGGVTRTRQIRDVAVDLGFMITVEDTGGAEIDTAAMAHLSLSTPQERRLHAI
AFHEWVTVRTAFNAPPVTGSHMGIPDGPGLGIDVDPSLLGKPFFEIG

Sequences:

>Translated_367_residues
MQIKSIKAYHVVQPFVDGPYRMSKGRQADAFDAVIVAITADSGLTGWGEMAPLGNFYSAAFPAGARAGVPEIAPHLIGHD
PRGLASIGRLMDTVFKGHPYIKSALDMACWDLAARAAGVPLVTMLGGRESDMAETYRVVTHGTLDQMAALAKRIIAEGCR
RLQVKVGGNVHDDIERVTTVASAVPKGTVIFCDANAGWTPYQARQFADATRGIDYTFEQPCTTIEENMSVRRMLDKPMVL
DESVTSLEEMLEIHRLGAADGLTLKISRLGGVTRTRQIRDVAVDLGFMITVEDTGGAEIDTAAMAHLSLSTPQERRLHAI
AFHEWVTVRTAFNAPPVTGSHMGIPDGPGLGIDVDPSLLGKPFFEIG
>Mature_367_residues
MQIKSIKAYHVVQPFVDGPYRMSKGRQADAFDAVIVAITADSGLTGWGEMAPLGNFYSAAFPAGARAGVPEIAPHLIGHD
PRGLASIGRLMDTVFKGHPYIKSALDMACWDLAARAAGVPLVTMLGGRESDMAETYRVVTHGTLDQMAALAKRIIAEGCR
RLQVKVGGNVHDDIERVTTVASAVPKGTVIFCDANAGWTPYQARQFADATRGIDYTFEQPCTTIEENMSVRRMLDKPMVL
DESVTSLEEMLEIHRLGAADGLTLKISRLGGVTRTRQIRDVAVDLGFMITVEDTGGAEIDTAAMAHLSLSTPQERRLHAI
AFHEWVTVRTAFNAPPVTGSHMGIPDGPGLGIDVDPSLLGKPFFEIG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=293, Percent_Identity=25.2559726962457, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39527; Mature: 39527

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKSIKAYHVVQPFVDGPYRMSKGRQADAFDAVIVAITADSGLTGWGEMAPLGNFYSAA
CCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHH
FPAGARAGVPEIAPHLIGHDPRGLASIGRLMDTVFKGHPYIKSALDMACWDLAARAAGVP
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
LVTMLGGRESDMAETYRVVTHGTLDQMAALAKRIIAEGCRRLQVKVGGNVHDDIERVTTV
EEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
ASAVPKGTVIFCDANAGWTPYQARQFADATRGIDYTFEQPCTTIEENMSVRRMLDKPMVL
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
DESVTSLEEMLEIHRLGAADGLTLKISRLGGVTRTRQIRDVAVDLGFMITVEDTGGAEID
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCHHHHHHHHHHEECEEEEEEECCCCCCCH
TAAMAHLSLSTPQERRLHAIAFHEWVTVRTAFNAPPVTGSHMGIPDGPGLGIDVDPSLLG
HHHHHHEECCCCHHHHHHHEEHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHC
KPFFEIG
CCHHCCC
>Mature Secondary Structure
MQIKSIKAYHVVQPFVDGPYRMSKGRQADAFDAVIVAITADSGLTGWGEMAPLGNFYSAA
CCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHH
FPAGARAGVPEIAPHLIGHDPRGLASIGRLMDTVFKGHPYIKSALDMACWDLAARAAGVP
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
LVTMLGGRESDMAETYRVVTHGTLDQMAALAKRIIAEGCRRLQVKVGGNVHDDIERVTTV
EEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
ASAVPKGTVIFCDANAGWTPYQARQFADATRGIDYTFEQPCTTIEENMSVRRMLDKPMVL
HHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCH
DESVTSLEEMLEIHRLGAADGLTLKISRLGGVTRTRQIRDVAVDLGFMITVEDTGGAEID
HHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCHHHHHHHHHHEECEEEEEEECCCCCCCH
TAAMAHLSLSTPQERRLHAIAFHEWVTVRTAFNAPPVTGSHMGIPDGPGLGIDVDPSLLG
HHHHHHEECCCCHHHHHHHEEHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHC
KPFFEIG
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]