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The map label for this gene is rfbB [C]

Identifier: 13475639

GI number: 13475639

Start: 5558563

End: 5559624

Strand: Reverse

Name: rfbB [C]

Synonym: mll6759

Alternate gene names: 13475639

Gene position: 5559624-5558563 (Counterclockwise)

Preceding gene: 13475640

Following gene: 13475632

Centisome position: 79.02

GC content: 62.34

Gene sequence:

>1062_bases
GTGTTGAAACTTGCACGCGCGGGGCCGATCGTCGTCACAGGTACCGCCGGATTCATCGGGTTTCATGTCGCGTCGCGGCT
TTTGCGGCGCGGCCTTGCCGTTATCGGCGTCGACAATTTTACGCCCTACTACGACGTCGGGCTGAAGGAGGCACGATTTG
CGCAGCTCTGTGCCGAACCGGGGTTCACTCCCATGCAGATGGACCTGGCGGACCAGGCGCTGGTGAAAGCACTGTTTTCC
GACTTCCAGCCATCGCACTTCGTGCATCTCGCCGCCCAGGCGGGCGTGCGTTATTCGCTCGCCGATCCGCATGCCTATGT
GCAGTCGAACATAGTCGCGTTTCTCAATGTTCTGGAGGGCTGCCGGCACGCCGGCGTCAGCCATCTCGTTTATGCCTCGT
CGAGTTCCGTCTACGGCGCCAATCGAAGCATACCGTTTTCCGAGCACCATGGCGCCAGTCATCCGGTCAGCTTCTATGCC
GCGACCAAAAGCGCAAACGAATGCATGGCGCATTCCTACAGCCACCTGTTCGGCTTGCCGGTGACCGGATTGCGCTTCTT
CACCGTGTATGGTCCTTGGGGCAGGCCCGACATGGCGGTCTATACGTTCACCCATGCCATCGCCGAGGGGCGGACGATCG
AGATTGCCAATGCCGGTCGCGTATGGCGAGATTTCACCTATATCGACGACATCGTAGAGGGGGTGGTTCGCGTGCTCGCC
GCGCCGCCTCGTCCGGATCCGGACTGGGACAGCCGCGCGGCTGCCCCCGCCACCAGCTCGGCGCCATATCGGATCTACAA
TATCGGCAATGACCGGCCCGAGGAAATCAACCGTCTGATCGCGATCATCGAGACTGCCCTTGGCCGCCGCGCCGTTCGCG
TCAACGTGCCTCTACCTCCAGGCGACGTCCTGAAAACCCGCGCCGACGTCAGCGATCTTCGCGGCGCTGTCGGCTTCGCG
CCGGCGACGGCACTCGAGGACGGCGTGCAACGTTTCGTGGAATGGTACCGGGATTTCCATGCAAGCCCAGCCGGCCGAGA
ACGAGCGTTCACTCAGGCATAG

Upstream 100 bases:

>100_bases
TGAGCGCGGAATCTCGCAATTGGAGTGACTTGTCGTGGTCGAAACAGATTCGACGCTCCACAAGCTGGCCGATACCGCTC
GTGCGCGGTCGGATGGTGCT

Downstream 100 bases:

>100_bases
GGCAACGGGCGACCGGTGGCGACCGGAAGACAACGCCGGTTCGTTTACCCAGGCCCATAGGATTTCACGCGAACGGAAGG
CGCTCGCACTGTCCCAGCGG

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MLKLARAGPIVVTGTAGFIGFHVASRLLRRGLAVIGVDNFTPYYDVGLKEARFAQLCAEPGFTPMQMDLADQALVKALFS
DFQPSHFVHLAAQAGVRYSLADPHAYVQSNIVAFLNVLEGCRHAGVSHLVYASSSSVYGANRSIPFSEHHGASHPVSFYA
ATKSANECMAHSYSHLFGLPVTGLRFFTVYGPWGRPDMAVYTFTHAIAEGRTIEIANAGRVWRDFTYIDDIVEGVVRVLA
APPRPDPDWDSRAAAPATSSAPYRIYNIGNDRPEEINRLIAIIETALGRRAVRVNVPLPPGDVLKTRADVSDLRGAVGFA
PATALEDGVQRFVEWYRDFHASPAGRERAFTQA

Sequences:

>Translated_353_residues
MLKLARAGPIVVTGTAGFIGFHVASRLLRRGLAVIGVDNFTPYYDVGLKEARFAQLCAEPGFTPMQMDLADQALVKALFS
DFQPSHFVHLAAQAGVRYSLADPHAYVQSNIVAFLNVLEGCRHAGVSHLVYASSSSVYGANRSIPFSEHHGASHPVSFYA
ATKSANECMAHSYSHLFGLPVTGLRFFTVYGPWGRPDMAVYTFTHAIAEGRTIEIANAGRVWRDFTYIDDIVEGVVRVLA
APPRPDPDWDSRAAAPATSSAPYRIYNIGNDRPEEINRLIAIIETALGRRAVRVNVPLPPGDVLKTRADVSDLRGAVGFA
PATALEDGVQRFVEWYRDFHASPAGRERAFTQA
>Mature_353_residues
MLKLARAGPIVVTGTAGFIGFHVASRLLRRGLAVIGVDNFTPYYDVGLKEARFAQLCAEPGFTPMQMDLADQALVKALFS
DFQPSHFVHLAAQAGVRYSLADPHAYVQSNIVAFLNVLEGCRHAGVSHLVYASSSSVYGANRSIPFSEHHGASHPVSFYA
ATKSANECMAHSYSHLFGLPVTGLRFFTVYGPWGRPDMAVYTFTHAIAEGRTIEIANAGRVWRDFTYIDDIVEGVVRVLA
APPRPDPDWDSRAAAPATSSAPYRIYNIGNDRPEEINRLIAIIETALGRRAVRVNVPLPPGDVLKTRADVSDLRGAVGFA
PATALEDGVQRFVEWYRDFHASPAGRERAFTQA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=353, Percent_Identity=24.929178470255, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=25, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI56237023, Length=366, Percent_Identity=24.0437158469945, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI56118217, Length=366, Percent_Identity=24.0437158469945, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI189083684, Length=366, Percent_Identity=24.0437158469945, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=23.728813559322, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994969, Length=352, Percent_Identity=25.2840909090909, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786974, Length=347, Percent_Identity=22.478386167147, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=27.247191011236, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=27.1186440677966, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=350, Percent_Identity=24.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=24.2603550295858, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=344, Percent_Identity=22.093023255814, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=26.1127596439169, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19923002, Length=349, Percent_Identity=24.3553008595989, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38516; Mature: 38516

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLARAGPIVVTGTAGFIGFHVASRLLRRGLAVIGVDNFTPYYDVGLKEARFAQLCAEP
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCHHHHHHHHHCC
GFTPMQMDLADQALVKALFSDFQPSHFVHLAAQAGVRYSLADPHAYVQSNIVAFLNVLEG
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCEEECCCCHHHHHHHHHHHHHHHHH
CRHAGVSHLVYASSSSVYGANRSIPFSEHHGASHPVSFYAATKSANECMAHSYSHLFGLP
HHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCC
VTGLRFFTVYGPWGRPDMAVYTFTHAIAEGRTIEIANAGRVWRDFTYIDDIVEGVVRVLA
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHC
APPRPDPDWDSRAAAPATSSAPYRIYNIGNDRPEEINRLIAIIETALGRRAVRVNVPLPP
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
GDVLKTRADVSDLRGAVGFAPATALEDGVQRFVEWYRDFHASPAGRERAFTQA
CHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKLARAGPIVVTGTAGFIGFHVASRLLRRGLAVIGVDNFTPYYDVGLKEARFAQLCAEP
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCCHHHHHHHHHCC
GFTPMQMDLADQALVKALFSDFQPSHFVHLAAQAGVRYSLADPHAYVQSNIVAFLNVLEG
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCEEECCCCHHHHHHHHHHHHHHHHH
CRHAGVSHLVYASSSSVYGANRSIPFSEHHGASHPVSFYAATKSANECMAHSYSHLFGLP
HHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCC
VTGLRFFTVYGPWGRPDMAVYTFTHAIAEGRTIEIANAGRVWRDFTYIDDIVEGVVRVLA
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHC
APPRPDPDWDSRAAAPATSSAPYRIYNIGNDRPEEINRLIAIIETALGRRAVRVNVPLPP
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCC
GDVLKTRADVSDLRGAVGFAPATALEDGVQRFVEWYRDFHASPAGRERAFTQA
CHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]