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The map label for this gene is gspE [C]

Identifier: 13475420

GI number: 13475420

Start: 5294220

End: 5295605

Strand: Direct

Name: gspE [C]

Synonym: mlr6483

Alternate gene names: 13475420

Gene position: 5294220-5295605 (Clockwise)

Preceding gene: 13475410

Following gene: 13475421

Centisome position: 75.24

GC content: 60.32

Gene sequence:

>1386_bases
ATGCTGGGGCGGTTCATCAAGGGTGGCGAGACGCGGATCGAGCCAAGAGTGGAGTCGATAGCGGTTGTCCCCGTTGCCAA
TGGGGCCTCGCCTGTCGCCGATCTCGAACCGCATGGCGACGAGTTCCTGGCGCTGAAAGTCGATCTGCATCGACATCTCA
TTGATCGCTTCAACCTCGCGTCGCTCGAAACTGCGTCGAAGGACGAGATCCTGAACGAAATCCGTCCGATCGTGCGCGAA
TTCGTGCGCGGTCGCAACGTTCCGCTGAACGCGCGCGAACTCGATCAGCTGACCAGCGACACGGCAGACGAAATGCTAGG
CCTGGGGCCGATCGAGCCATTGCTGAAGGACGATTCGATTGCAGACATTCTGATCAATACCCACAAGCGTGTCTTCATCG
AGCGATATGGCGTGATCGAGGAAACCGCGATCCGCTTCCGCGACGAGGCCCATCTCCTGCGCGTCATCAACAAGATCGTC
TCGGCGATAGGCCGGCGTGTCGACGAATCGGTACCGATGGTCGATGCCCGGCTAGCAGACGGCTCACGCGTTAACATCGC
GGTGCGGCCGATCTCAGTGGACGGTCCGCTGGTCTCCATCCGTAAATTTTCCAAGAACCCTTATTCTCTCGAACGTCTGA
TGGCGTTCAACTCGATCCGACAGCCGATGGTCGATCTGCTTCGCATCGCGGTACAGTCGCGCAAGTCGATCCTGGTTTCC
GGCGGCACCGGCAGCGGCAAGACGACCTTGCTCAACGCGTTGTCCAGCTACATCCCGTCGAGGGAACGCCTGATCACCAT
CGAGGATGCGGCGGAACTGCAACTGCAGCAACCGCATGTCGGACGGCTGGAGACGCGCCCGCCCAATGTCGAGGGCAAAG
GCGAGGTTCGCCAGCGCGAACTGCTCAAGAACGCACTGCGCATGCGACCGGACCGCATCATCGTCGGCGAGGTGCGCGGC
GAGGAGGCCTTCGATATGCTGCAGGCCATGAACACCGGCCATGAAGGCTCGATGACAACCATCCACGCCAACACGCCCCG
CGATGCGATCTCGCGGCTCGAGCAGATGGTCGGCATGGCCGGCATGCCGATGACCAACGATTCCATTCGCGCGCAGATCG
CCTCGGCCATCGATATCATCGTACAGACACAACGGCTCTCGGACGGCGGCAGGCGCGTCACTTCCATATCGGAACTGACC
GGCATGGAAGGCAACGTTGTCCAGCTTCAGGAAATCTATCACTTCGTTCGCCGCGATGTCCGCGCCGATGGCACGATTGT
CGGCGAATTTCGCGCCACCGGTGTACGGCCGCGTTTTGCCACTGAAGCCGCGGCAATGGGGTATCATTTCGCAAAAGATG
CGTTCAATCCGCAGGTTCCACTTTGA

Upstream 100 bases:

>100_bases
TGTTGAAAGTATTGCCTTGGCGTGAGGCGTCCCGGCGCTTGTGCGGCGCTTGGTTAACGTTTAGTTTCCGCTAATATGCG
CTGGAGGGGTATAAGGGATT

Downstream 100 bases:

>100_bases
TGCCGACCGGTCAGACCCTCCTCTACTTCATCTATGTGCTGGCGGCGGCATCCGTTATTCTCGCCGCCGAATCCTTCTTT
CTCTCAATCGCCGGACGGCG

Product: secretory protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLGRFIKGGETRIEPRVESIAVVPVANGASPVADLEPHGDEFLALKVDLHRHLIDRFNLASLETASKDEILNEIRPIVRE
FVRGRNVPLNARELDQLTSDTADEMLGLGPIEPLLKDDSIADILINTHKRVFIERYGVIEETAIRFRDEAHLLRVINKIV
SAIGRRVDESVPMVDARLADGSRVNIAVRPISVDGPLVSIRKFSKNPYSLERLMAFNSIRQPMVDLLRIAVQSRKSILVS
GGTGSGKTTLLNALSSYIPSRERLITIEDAAELQLQQPHVGRLETRPPNVEGKGEVRQRELLKNALRMRPDRIIVGEVRG
EEAFDMLQAMNTGHEGSMTTIHANTPRDAISRLEQMVGMAGMPMTNDSIRAQIASAIDIIVQTQRLSDGGRRVTSISELT
GMEGNVVQLQEIYHFVRRDVRADGTIVGEFRATGVRPRFATEAAAMGYHFAKDAFNPQVPL

Sequences:

>Translated_461_residues
MLGRFIKGGETRIEPRVESIAVVPVANGASPVADLEPHGDEFLALKVDLHRHLIDRFNLASLETASKDEILNEIRPIVRE
FVRGRNVPLNARELDQLTSDTADEMLGLGPIEPLLKDDSIADILINTHKRVFIERYGVIEETAIRFRDEAHLLRVINKIV
SAIGRRVDESVPMVDARLADGSRVNIAVRPISVDGPLVSIRKFSKNPYSLERLMAFNSIRQPMVDLLRIAVQSRKSILVS
GGTGSGKTTLLNALSSYIPSRERLITIEDAAELQLQQPHVGRLETRPPNVEGKGEVRQRELLKNALRMRPDRIIVGEVRG
EEAFDMLQAMNTGHEGSMTTIHANTPRDAISRLEQMVGMAGMPMTNDSIRAQIASAIDIIVQTQRLSDGGRRVTSISELT
GMEGNVVQLQEIYHFVRRDVRADGTIVGEFRATGVRPRFATEAAAMGYHFAKDAFNPQVPL
>Mature_461_residues
MLGRFIKGGETRIEPRVESIAVVPVANGASPVADLEPHGDEFLALKVDLHRHLIDRFNLASLETASKDEILNEIRPIVRE
FVRGRNVPLNARELDQLTSDTADEMLGLGPIEPLLKDDSIADILINTHKRVFIERYGVIEETAIRFRDEAHLLRVINKIV
SAIGRRVDESVPMVDARLADGSRVNIAVRPISVDGPLVSIRKFSKNPYSLERLMAFNSIRQPMVDLLRIAVQSRKSILVS
GGTGSGKTTLLNALSSYIPSRERLITIEDAAELQLQQPHVGRLETRPPNVEGKGEVRQRELLKNALRMRPDRIIVGEVRG
EEAFDMLQAMNTGHEGSMTTIHANTPRDAISRLEQMVGMAGMPMTNDSIRAQIASAIDIIVQTQRLSDGGRRVTSISELT
GMEGNVVQLQEIYHFVRRDVRADGTIVGEFRATGVRPRFATEAAAMGYHFAKDAFNPQVPL

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=277, Percent_Identity=31.7689530685921, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 51144; Mature: 51144

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRFIKGGETRIEPRVESIAVVPVANGASPVADLEPHGDEFLALKVDLHRHLIDRFNLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCH
SLETASKDEILNEIRPIVREFVRGRNVPLNARELDQLTSDTADEMLGLGPIEPLLKDDSI
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCH
ADILINTHKRVFIERYGVIEETAIRFRDEAHLLRVINKIVSAIGRRVDESVPMVDARLAD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GSRVNIAVRPISVDGPLVSIRKFSKNPYSLERLMAFNSIRQPMVDLLRIAVQSRKSILVS
CCEEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GGTGSGKTTLLNALSSYIPSRERLITIEDAAELQLQQPHVGRLETRPPNVEGKGEVRQRE
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHEECCCCCCCCCCCCCCCCCCCHHHHHH
LLKNALRMRPDRIIVGEVRGEEAFDMLQAMNTGHEGSMTTIHANTPRDAISRLEQMVGMA
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
GMPMTNDSIRAQIASAIDIIVQTQRLSDGGRRVTSISELTGMEGNVVQLQEIYHFVRRDV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHH
RADGTIVGEFRATGVRPRFATEAAAMGYHFAKDAFNPQVPL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLGRFIKGGETRIEPRVESIAVVPVANGASPVADLEPHGDEFLALKVDLHRHLIDRFNLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCCCH
SLETASKDEILNEIRPIVREFVRGRNVPLNARELDQLTSDTADEMLGLGPIEPLLKDDSI
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCH
ADILINTHKRVFIERYGVIEETAIRFRDEAHLLRVINKIVSAIGRRVDESVPMVDARLAD
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GSRVNIAVRPISVDGPLVSIRKFSKNPYSLERLMAFNSIRQPMVDLLRIAVQSRKSILVS
CCEEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GGTGSGKTTLLNALSSYIPSRERLITIEDAAELQLQQPHVGRLETRPPNVEGKGEVRQRE
CCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHEECCCCCCCCCCCCCCCCCCCHHHHHH
LLKNALRMRPDRIIVGEVRGEEAFDMLQAMNTGHEGSMTTIHANTPRDAISRLEQMVGMA
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
GMPMTNDSIRAQIASAIDIIVQTQRLSDGGRRVTSISELTGMEGNVVQLQEIYHFVRRDV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHH
RADGTIVGEFRATGVRPRFATEAAAMGYHFAKDAFNPQVPL
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]