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The map label for this gene is lacF [H]

Identifier: 13475384

GI number: 13475384

Start: 5258500

End: 5259360

Strand: Direct

Name: lacF [H]

Synonym: mlr6436

Alternate gene names: 13475384

Gene position: 5258500-5259360 (Clockwise)

Preceding gene: 13475383

Following gene: 13475385

Centisome position: 74.74

GC content: 61.21

Gene sequence:

>861_bases
ATGCGCAACCGCAATGCCACGGCCTATCGTTTCCTGGCGCCCTATCTGCTGATCTTCTCCGTCTTCTGGGTGTGGCCGAT
CATCTCGTCGTTCATGATCTCCTTCCAGGCGACACGCACCGTGCCCTGGCGTTTCGCACCGGGCTTCAACTGGGGCCGGC
TGATTGGCGATCCGGCCTTCTTCAACGCGCTGAAGAACACGCTGCTCATCCTCGTCATCCAGGTGCCGGTGATGATCGCG
CTGGCCATGGTGATGGCCGTGCTTTTGAATTCGCCGCTCCTGAAGGCGAGAGGCCTCTACCGCTTCGCCTTCTTTGCTCC
CGTCGTCGTCGGCGAGGTCGCTTATTCGGCGGTATTCCGGCTGATGTTCAGCCTCGATTTCGGTATCGTCAACAAGCTCC
TGAACAGCGTCGGGCTGCCCAAGATCGACTGGTTCTCCAATGTCACGCCGGCCATGGCGCTGATCATGATCGCGGTCACC
TGGCGCTGGGCCGGCTACAACGCCATCATCCTTCTGGCCGGCATGCAATCCATCCCCGAGGATGTCTATGAGGCGGCGAC
GCTCGACCGCGTCAGCAAGCTCAAGCAGTTCTTCTACATCACCTTGCCGCTCTTGAAGCCGGTCATCGTGTTCTGCCTGG
TTCTCTCGATCATCGGCACCATGCAATTGTTCACCGAGCCCTTCCTGATCACCGAGCGCGGCGGGCCGGGTGGCGGCACC
GAGACACTCGGCCTGCTGCTCTACCGCCAGGGGTTCCGGTCGCTCAATTTCGGCTATGCCTCGGCCGTTGCCTACACCAT
GGCGGCGCTGGCGATCGCGATCACGCTGGTGCAGCTGTGGCTGACGAGGGAAAAGCAATGA

Upstream 100 bases:

>100_bases
AAATCATAAACTCCCAAGGACGGGGTCCGGGACGGCTCTTGCCGCCCGGACCTCCCGCCTGCGAGCTTGTCTGAAACACC
GGATATGAGGGAGGAGCAGG

Downstream 100 bases:

>100_bases
GTTCGCTCTCCTCCGCCCGCCTGAGGCGCAGCATCGCGCTGCATCTTTTCCTCACCCCGCTGGCGCTGATCTGGCTGTTC
CCGCTGTGGATGATGGTGGT

Product: ABC transporter sugar permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MRNRNATAYRFLAPYLLIFSVFWVWPIISSFMISFQATRTVPWRFAPGFNWGRLIGDPAFFNALKNTLLILVIQVPVMIA
LAMVMAVLLNSPLLKARGLYRFAFFAPVVVGEVAYSAVFRLMFSLDFGIVNKLLNSVGLPKIDWFSNVTPAMALIMIAVT
WRWAGYNAIILLAGMQSIPEDVYEAATLDRVSKLKQFFYITLPLLKPVIVFCLVLSIIGTMQLFTEPFLITERGGPGGGT
ETLGLLLYRQGFRSLNFGYASAVAYTMAALAIAITLVQLWLTREKQ

Sequences:

>Translated_286_residues
MRNRNATAYRFLAPYLLIFSVFWVWPIISSFMISFQATRTVPWRFAPGFNWGRLIGDPAFFNALKNTLLILVIQVPVMIA
LAMVMAVLLNSPLLKARGLYRFAFFAPVVVGEVAYSAVFRLMFSLDFGIVNKLLNSVGLPKIDWFSNVTPAMALIMIAVT
WRWAGYNAIILLAGMQSIPEDVYEAATLDRVSKLKQFFYITLPLLKPVIVFCLVLSIIGTMQLFTEPFLITERGGPGGGT
ETLGLLLYRQGFRSLNFGYASAVAYTMAALAIAITLVQLWLTREKQ
>Mature_286_residues
MRNRNATAYRFLAPYLLIFSVFWVWPIISSFMISFQATRTVPWRFAPGFNWGRLIGDPAFFNALKNTLLILVIQVPVMIA
LAMVMAVLLNSPLLKARGLYRFAFFAPVVVGEVAYSAVFRLMFSLDFGIVNKLLNSVGLPKIDWFSNVTPAMALIMIAVT
WRWAGYNAIILLAGMQSIPEDVYEAATLDRVSKLKQFFYITLPLLKPVIVFCLVLSIIGTMQLFTEPFLITERGGPGGGT
ETLGLLLYRQGFRSLNFGYASAVAYTMAALAIAITLVQLWLTREKQ

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=286, Percent_Identity=26.5734265734266, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790465, Length=229, Percent_Identity=30.1310043668122, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787570, Length=274, Percent_Identity=27.3722627737226, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32047; Mature: 32047

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNRNATAYRFLAPYLLIFSVFWVWPIISSFMISFQATRTVPWRFAPGFNWGRLIGDPAF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHH
FNALKNTLLILVIQVPVMIALAMVMAVLLNSPLLKARGLYRFAFFAPVVVGEVAYSAVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMFSLDFGIVNKLLNSVGLPKIDWFSNVTPAMALIMIAVTWRWAGYNAIILLAGMQSIPE
HHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHEECCHHHHHH
DVYEAATLDRVSKLKQFFYITLPLLKPVIVFCLVLSIIGTMQLFTEPFLITERGGPGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCH
ETLGLLLYRQGFRSLNFGYASAVAYTMAALAIAITLVQLWLTREKQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNRNATAYRFLAPYLLIFSVFWVWPIISSFMISFQATRTVPWRFAPGFNWGRLIGDPAF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCHHH
FNALKNTLLILVIQVPVMIALAMVMAVLLNSPLLKARGLYRFAFFAPVVVGEVAYSAVFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMFSLDFGIVNKLLNSVGLPKIDWFSNVTPAMALIMIAVTWRWAGYNAIILLAGMQSIPE
HHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCHHHEECCHHHHHH
DVYEAATLDRVSKLKQFFYITLPLLKPVIVFCLVLSIIGTMQLFTEPFLITERGGPGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCH
ETLGLLLYRQGFRSLNFGYASAVAYTMAALAIAITLVQLWLTREKQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]