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The map label for this gene is lacE [H]

Identifier: 13475383

GI number: 13475383

Start: 5257137

End: 5258408

Strand: Direct

Name: lacE [H]

Synonym: mlr6435

Alternate gene names: 13475383

Gene position: 5257137-5258408 (Clockwise)

Preceding gene: 13475378

Following gene: 13475384

Centisome position: 74.72

GC content: 61.48

Gene sequence:

>1272_bases
ATGCGCATCACACTGTCTTGCGCCGCGCTCGCGCTTGTCCTGGGCTCAGCCCCGGCCTTTGCGCAATCCGGCGAAATCAC
CATCTGGAGCTGGAATGTCGCCGCTTCGTCGCTGAAGGCGACGGTTGCCGGCTTCAACAAGCTGCATCCCGACATCAAGG
TCACGGTCCAGGATCTCGGCAACCAGCCGACCTATGACAAGTCGATCGCCGGCTGTGCCGCCGGTGGCGTCGGCCTGCCT
GACATCGTTACCATCGAGAATGGCGAGGCCGAGAATTACTGGAGCCAGTTCCCCGATTGCTTCGTCGACCTGCATACGCT
GGGTTATACGTCGGAAGACCAGAAGAAATTCCCCGATTTCAAGCGCACCGAACTCGAGGTCGGCGACAAGGCCTATGCCA
TGCCGTGGGATTCCGGCCCGGTCGCCGCTTTCTATCGCCGCGACTTCTACGAGAAGGCCGGGGTAGATCCGGCTTCGATC
AAGACCTGGGACGATTTCATCGCCGCCGGCAAGAAGATCCAGGCCGCCAATCCGGGCGTCTCGATGACCAACGCCGATTT
CAACGGCGACACCGAATTCTTCCGCATGATCTCCAACGAGCAGGGCTGCGCCTACTTCGCCGAGGATGGCCAGTCGATCA
CGGTCAGCGAACCCAAATGCGTCGACGCCCTGACCAAGGTCAAGGAGATGAAGGACGCCGGCATCGTCTCGTCCGCCGAC
TGGGGCACCAAGATCACCAACAACACGGCAGGCACCGTCGCCACCCAGGTCTATGGCGGTTGGTATGAAGGCACCATCCG
CACCGAATCGGCCGGCGAAAACGGCAAATGGGGCGTTTATCTGATGCCGAGCCTAACCGCCGACGGTCCCCGCGCCGCCA
ATCTCGGCGGCTCGTCGCTGGCCATCACCTCGGCTTCGAAGAACAAGGAAGCGGCATACGAATACCTGAAATACACGCTC
GGAACCAATGAAGGCCAGATCACCATGCTGAAGGAGTTCGGCCTCGTCCCCTCGCTGGTCTCGGCGCTGAATGACCCCTA
TGTCAGCCAAGGCCTGCCCTACTGGGGTGGCCAGGCGGTGTGGAAGGATATTCTGGGCACCTTGCCCAAGGTCGTCCCCA
GCCGCGGCACGCAGTTCCAGAGCGATGCCGAAATCATCGTCCGTGCGGTGCAGACCAAATATCTGGCCGGCGGCTATCCC
GATGCTAAGGCCGCACTCGACGACGCCGCAAAACAGATCGCCGCGGCGACCGGACTGCCGGTCAAATCATAA

Upstream 100 bases:

>100_bases
CTTTCCGCCAATTCCGTATTCCCTTTGTCGGCGACAAGCCTTCCGCGCCGAATAGTGTAGCCACGCTCGCAGAGAGAGCA
AATGAAATGGGAGGATTTCA

Downstream 100 bases:

>100_bases
ACTCCCAAGGACGGGGTCCGGGACGGCTCTTGCCGCCCGGACCTCCCGCCTGCGAGCTTGTCTGAAACACCGGATATGAG
GGAGGAGCAGGATGCGCAAC

Product: ABC transporter sugar binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: LBP [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRITLSCAALALVLGSAPAFAQSGEITIWSWNVAASSLKATVAGFNKLHPDIKVTVQDLGNQPTYDKSIAGCAAGGVGLP
DIVTIENGEAENYWSQFPDCFVDLHTLGYTSEDQKKFPDFKRTELEVGDKAYAMPWDSGPVAAFYRRDFYEKAGVDPASI
KTWDDFIAAGKKIQAANPGVSMTNADFNGDTEFFRMISNEQGCAYFAEDGQSITVSEPKCVDALTKVKEMKDAGIVSSAD
WGTKITNNTAGTVATQVYGGWYEGTIRTESAGENGKWGVYLMPSLTADGPRAANLGGSSLAITSASKNKEAAYEYLKYTL
GTNEGQITMLKEFGLVPSLVSALNDPYVSQGLPYWGGQAVWKDILGTLPKVVPSRGTQFQSDAEIIVRAVQTKYLAGGYP
DAKAALDDAAKQIAAATGLPVKS

Sequences:

>Translated_423_residues
MRITLSCAALALVLGSAPAFAQSGEITIWSWNVAASSLKATVAGFNKLHPDIKVTVQDLGNQPTYDKSIAGCAAGGVGLP
DIVTIENGEAENYWSQFPDCFVDLHTLGYTSEDQKKFPDFKRTELEVGDKAYAMPWDSGPVAAFYRRDFYEKAGVDPASI
KTWDDFIAAGKKIQAANPGVSMTNADFNGDTEFFRMISNEQGCAYFAEDGQSITVSEPKCVDALTKVKEMKDAGIVSSAD
WGTKITNNTAGTVATQVYGGWYEGTIRTESAGENGKWGVYLMPSLTADGPRAANLGGSSLAITSASKNKEAAYEYLKYTL
GTNEGQITMLKEFGLVPSLVSALNDPYVSQGLPYWGGQAVWKDILGTLPKVVPSRGTQFQSDAEIIVRAVQTKYLAGGYP
DAKAALDDAAKQIAAATGLPVKS
>Mature_423_residues
MRITLSCAALALVLGSAPAFAQSGEITIWSWNVAASSLKATVAGFNKLHPDIKVTVQDLGNQPTYDKSIAGCAAGGVGLP
DIVTIENGEAENYWSQFPDCFVDLHTLGYTSEDQKKFPDFKRTELEVGDKAYAMPWDSGPVAAFYRRDFYEKAGVDPASI
KTWDDFIAAGKKIQAANPGVSMTNADFNGDTEFFRMISNEQGCAYFAEDGQSITVSEPKCVDALTKVKEMKDAGIVSSAD
WGTKITNNTAGTVATQVYGGWYEGTIRTESAGENGKWGVYLMPSLTADGPRAANLGGSSLAITSASKNKEAAYEYLKYTL
GTNEGQITMLKEFGLVPSLVSALNDPYVSQGLPYWGGQAVWKDILGTLPKVVPSRGTQFQSDAEIIVRAVQTKYLAGGYP
DAKAALDDAAKQIAAATGLPVKS

Specific function: Part of the binding-protein-dependent transport system for lactose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45187; Mature: 45187

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLSCAALALVLGSAPAFAQSGEITIWSWNVAASSLKATVAGFNKLHPDIKVTVQDLG
CEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCC
NQPTYDKSIAGCAAGGVGLPDIVTIENGEAENYWSQFPDCFVDLHTLGYTSEDQKKFPDF
CCCCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCHHHEEHHHCCCCCCHHHHCCCC
KRTELEVGDKAYAMPWDSGPVAAFYRRDFYEKAGVDPASIKTWDDFIAAGKKIQAANPGV
CCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCC
SMTNADFNGDTEFFRMISNEQGCAYFAEDGQSITVSEPKCVDALTKVKEMKDAGIVSSAD
EEECCCCCCCHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCEECCC
WGTKITNNTAGTVATQVYGGWYEGTIRTESAGENGKWGVYLMPSLTADGPRAANLGGSSL
CCCEECCCCCCCEEHHHCCCEEECEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
AITSASKNKEAAYEYLKYTLGTNEGQITMLKEFGLVPSLVSALNDPYVSQGLPYWGGQAV
EEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHCCCHHHCCCCCCCCHHH
WKDILGTLPKVVPSRGTQFQSDAEIIVRAVQTKYLAGGYPDAKAALDDAAKQIAAATGLP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
VKS
CCC
>Mature Secondary Structure
MRITLSCAALALVLGSAPAFAQSGEITIWSWNVAASSLKATVAGFNKLHPDIKVTVQDLG
CEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCC
NQPTYDKSIAGCAAGGVGLPDIVTIENGEAENYWSQFPDCFVDLHTLGYTSEDQKKFPDF
CCCCCCHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCHHHEEHHHCCCCCCHHHHCCCC
KRTELEVGDKAYAMPWDSGPVAAFYRRDFYEKAGVDPASIKTWDDFIAAGKKIQAANPGV
CCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEECCCCC
SMTNADFNGDTEFFRMISNEQGCAYFAEDGQSITVSEPKCVDALTKVKEMKDAGIVSSAD
EEECCCCCCCHHHHHHHCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCEECCC
WGTKITNNTAGTVATQVYGGWYEGTIRTESAGENGKWGVYLMPSLTADGPRAANLGGSSL
CCCEECCCCCCCEEHHHCCCEEECEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCEE
AITSASKNKEAAYEYLKYTLGTNEGQITMLKEFGLVPSLVSALNDPYVSQGLPYWGGQAV
EEEECCCCHHHHHHHHHHHCCCCCCCEEEHHHHCCHHHHHHHHCCCHHHCCCCCCCCHHH
WKDILGTLPKVVPSRGTQFQSDAEIIVRAVQTKYLAGGYPDAKAALDDAAKQIAAATGLP
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
VKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630315; 2318800 [H]