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The map label for this gene is nifB [H]

Identifier: 13474877

GI number: 13474877

Start: 4728641

End: 4730122

Strand: Reverse

Name: nifB [H]

Synonym: mll5855

Alternate gene names: 13474877

Gene position: 4730122-4728641 (Counterclockwise)

Preceding gene: 13474878

Following gene: 13474876

Centisome position: 67.23

GC content: 61.2

Gene sequence:

>1482_bases
ATGTCCGCACCGATGATTTCGCTACAGGGCCTTTCCGGCACGACAGTCTTCGATCAGTCGCCGGCGAGAGCGAAATCCGG
TGGCTGCGCATCTTCATCCTGCGGCTCCTCCGCGAAGCCGCACGAGATGGACTCGGCCGTCTGGGAGAAGATCAAGGATC
ATCCCTGCTTTTCAGAGGAAGCGCACCACTATTTCGCGCGCATGCATGTGGCGGTCGCCCCAGCCTGCAATATTCAATGC
AACTACTGCAATCGCAAATATGACTGCGCCAACGAAAGCCGGCCTGGGATCGTTTCGGAAAGGCTGACGCCCGACCAGGC
GCTGCGCAAGGTGATCGGGGTTGCCAACGAAGTGCCGCAGCTTTCCGTGCTTGGCATCGCCGGACCGGGCGATGCCTGTT
ACGACTGGAAGAACACAAAGGCGACATTCCAACGGGTCGCCAAGGAAATCGGCGACATCAAGCTGTGCATCTCAACCAAC
GGGCTCGCGCTGCCAGACCGCGTCGCCGAGCTTGCCGAAATGAATGTCGATCACGTGACGATCACCATCAACATGGTCGA
CCCGCGGATCGGTGCCAAGATCTATCCGTGGATCTTCTATGGCAACCGCCGTTATACCGGCGTTGAGGCCGCCACGATCC
TGCACGAACGACAGATGTCGGGGCTGGAGATGCTGACGGCGCGCGGCATCCTCACCAAGATCAATTCGGTAATGATCCCC
GGCGTAAACGATGAGCACCTGATCGAGGTGAATAAATGGGTCAAGGAGCGCGGCGCGTTCCTGCACAATGTCATGCCGCT
GATTTCCGACCCGGCGCACGGTACGCATTACGGCCTGAGTGGGCAGCGCGGCCCCAAGGCAATGGAGCTGAAGGCCCTTC
AGGATCGTCTCGAAGGCGGCGCCAAGTTGATGCGCCACTGCCGGCAGTGCCGGGCCGATGCTGTCGGCCTGCTCGGCGAG
GATCGTGGCCAGGAATTCACGCTCGACCGGCTTCCCGACAAGGTCACGTACGACGCCAGCAAGCGCGAGACATATCGGGC
AGTGGTCGCGCGCGAGCGGGGCGATCGCCTGGCGGCCAAGAGCGAGGCGCTCGGGATGGTCAAGACCGCAGGCTCCGGCA
AATCGCTTCTGGTAGCGGTGGCGACCAAGGGTGGCGGCCGCATCAACGAGCATTTCGGCCATGCGAAGGAATTCCAGGTT
TATGAGGCCTCTCCGAAAGGGATCAGTTTCGTCGGTCATCGCAAGGTCGAACAGTATTGCCTCGGCGGCCGGGCCGAGGA
CAAGAGCCTCGACGGCGTCATCTCTACGCTCGAAGGCGTAGACATCGTGCTGTGCGCGAGAATTGGAAATTGCCCCGAGG
ATCGTCTCAAGGAAGGTGGGATCCGAGCAACGGAGGCTTACGGGTATGACTACATCGAGACCGCGATCGGCGCGCTTTAC
GCCGCCGAGTTTGGGAGTGAGCCACTGGCTGCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCCGACGTAAAAGCCAACATGACGGATAAGACCGCTTTTGGGGTTGGCATATCTCTTGCGCTCTGAAGTGCGATGTTCAC
ACCACGCACGGAGCCGCTCC

Downstream 100 bases:

>100_bases
GCCGCCTCATTCTAACCGAACCGGAGTTGAAAATGGCCTTTAAGATCATCGCTTCCCAATGCACCCAGTGCGGTGCCTGT
GAGTTTGAATGCCCTTCGGG

Product: nitrogen fixation protein nifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSAPMISLQGLSGTTVFDQSPARAKSGGCASSSCGSSAKPHEMDSAVWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGIVSERLTPDQALRKVIGVANEVPQLSVLGIAGPGDACYDWKNTKATFQRVAKEIGDIKLCISTN
GLALPDRVAELAEMNVDHVTITINMVDPRIGAKIYPWIFYGNRRYTGVEAATILHERQMSGLEMLTARGILTKINSVMIP
GVNDEHLIEVNKWVKERGAFLHNVMPLISDPAHGTHYGLSGQRGPKAMELKALQDRLEGGAKLMRHCRQCRADAVGLLGE
DRGQEFTLDRLPDKVTYDASKRETYRAVVARERGDRLAAKSEALGMVKTAGSGKSLLVAVATKGGGRINEHFGHAKEFQV
YEASPKGISFVGHRKVEQYCLGGRAEDKSLDGVISTLEGVDIVLCARIGNCPEDRLKEGGIRATEAYGYDYIETAIGALY
AAEFGSEPLAATA

Sequences:

>Translated_493_residues
MSAPMISLQGLSGTTVFDQSPARAKSGGCASSSCGSSAKPHEMDSAVWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQC
NYCNRKYDCANESRPGIVSERLTPDQALRKVIGVANEVPQLSVLGIAGPGDACYDWKNTKATFQRVAKEIGDIKLCISTN
GLALPDRVAELAEMNVDHVTITINMVDPRIGAKIYPWIFYGNRRYTGVEAATILHERQMSGLEMLTARGILTKINSVMIP
GVNDEHLIEVNKWVKERGAFLHNVMPLISDPAHGTHYGLSGQRGPKAMELKALQDRLEGGAKLMRHCRQCRADAVGLLGE
DRGQEFTLDRLPDKVTYDASKRETYRAVVARERGDRLAAKSEALGMVKTAGSGKSLLVAVATKGGGRINEHFGHAKEFQV
YEASPKGISFVGHRKVEQYCLGGRAEDKSLDGVISTLEGVDIVLCARIGNCPEDRLKEGGIRATEAYGYDYIETAIGALY
AAEFGSEPLAATA
>Mature_492_residues
SAPMISLQGLSGTTVFDQSPARAKSGGCASSSCGSSAKPHEMDSAVWEKIKDHPCFSEEAHHYFARMHVAVAPACNIQCN
YCNRKYDCANESRPGIVSERLTPDQALRKVIGVANEVPQLSVLGIAGPGDACYDWKNTKATFQRVAKEIGDIKLCISTNG
LALPDRVAELAEMNVDHVTITINMVDPRIGAKIYPWIFYGNRRYTGVEAATILHERQMSGLEMLTARGILTKINSVMIPG
VNDEHLIEVNKWVKERGAFLHNVMPLISDPAHGTHYGLSGQRGPKAMELKALQDRLEGGAKLMRHCRQCRADAVGLLGED
RGQEFTLDRLPDKVTYDASKRETYRAVVARERGDRLAAKSEALGMVKTAGSGKSLLVAVATKGGGRINEHFGHAKEFQVY
EASPKGISFVGHRKVEQYCLGGRAEDKSLDGVISTLEGVDIVLCARIGNCPEDRLKEGGIRATEAYGYDYIETAIGALYA
AEFGSEPLAATA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53570; Mature: 53438

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPMISLQGLSGTTVFDQSPARAKSGGCASSSCGSSAKPHEMDSAVWEKIKDHPCFSEE
CCCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGIVSERLTPDQALRKVIGVANEVPQ
HHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCC
LSVLGIAGPGDACYDWKNTKATFQRVAKEIGDIKLCISTNGLALPDRVAELAEMNVDHVT
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
ITINMVDPRIGAKIYPWIFYGNRRYTGVEAATILHERQMSGLEMLTARGILTKINSVMIP
EEEEEECCCCCCEEEEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
GVNDEHLIEVNKWVKERGAFLHNVMPLISDPAHGTHYGLSGQRGPKAMELKALQDRLEGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AKLMRHCRQCRADAVGLLGEDRGQEFTLDRLPDKVTYDASKRETYRAVVARERGDRLAAK
HHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHH
SEALGMVKTAGSGKSLLVAVATKGGGRINEHFGHAKEFQVYEASPKGISFVGHRKVEQYC
HHHCCEEEECCCCCEEEEEEEECCCCCCHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHH
LGGRAEDKSLDGVISTLEGVDIVLCARIGNCPEDRLKEGGIRATEAYGYDYIETAIGALY
CCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEHHCCCHHHHHHHHHHH
AAEFGSEPLAATA
HHHHCCCCCCCCC
>Mature Secondary Structure 
SAPMISLQGLSGTTVFDQSPARAKSGGCASSSCGSSAKPHEMDSAVWEKIKDHPCFSEE
CCCCEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHH
AHHYFARMHVAVAPACNIQCNYCNRKYDCANESRPGIVSERLTPDQALRKVIGVANEVPQ
HHHHHHHHHHEECCCCCEEECCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCC
LSVLGIAGPGDACYDWKNTKATFQRVAKEIGDIKLCISTNGLALPDRVAELAEMNVDHVT
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEE
ITINMVDPRIGAKIYPWIFYGNRRYTGVEAATILHERQMSGLEMLTARGILTKINSVMIP
EEEEEECCCCCCEEEEEEEECCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
GVNDEHLIEVNKWVKERGAFLHNVMPLISDPAHGTHYGLSGQRGPKAMELKALQDRLEGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AKLMRHCRQCRADAVGLLGEDRGQEFTLDRLPDKVTYDASKRETYRAVVARERGDRLAAK
HHHHHHHHHHHHHHHCCCCCCCCCEEEHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHH
SEALGMVKTAGSGKSLLVAVATKGGGRINEHFGHAKEFQVYEASPKGISFVGHRKVEQYC
HHHCCEEEECCCCCEEEEEEEECCCCCCHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHH
LGGRAEDKSLDGVISTLEGVDIVLCARIGNCPEDRLKEGGIRATEAYGYDYIETAIGALY
CCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEHHCCCHHHHHHHHHHH
AAEFGSEPLAATA
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]